Nuno Mendonça

Centro De Medicina Veterinária Anjos De Assis

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Gabriela Silva

Universidade Federal dos Vales do Jequitinhonha e Mucuri

Manuela Caniça

National Institute of Health Dr. Ricardo Jorge

Eugénia Ferreira

Centro Universitário Anhanguera

Vera Calhau

University of Coimbra

Carlos A. Molina

Vall d'Hebron Hospital Universitari

JÁ

José Álvarez‐Sabín

Vall d'Hebron Hospital Universitari

Gustavo Cordeiro

Hospitais da Universidade de Coimbra

Marta Rubiera

Vall d'Hebron Hospital Universitari

Rui Figueiredo

Centro Hospitalar de Entre o Douro e Vouga E.P.E.

Marc Ribó

Vall d'Hebron Hospital Universitari

Cooperative Institutions

University of Coimbra

Hospitais da Universidade de Coimbra

University of Lisbon

Universidade Nova de Lisboa

Universitat Autònoma de Barcelona

Vall d'Hebron Hospital Universitari

Universidade do Porto

Vall d'Hebron Institut de Recerca

Centro de Neurociências e Biologia Celular

National Institute of Health Dr. Ricardo Jorge

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Virulence Characterization of Salmonella enterica by a New Microarray: Detection and Evaluation of the Cytolethal Distending Toxin Gene Activity in the Unusual Host S. Typhimurium

PLoS ONE (2015)

Rui Figueiredo Roderick M. Card Carla Nunes Manal AbuOun Mary C. Bagnall

Salmonella enterica is a zoonotic foodborne pathogen that causes acute gastroenteritis in humans. We assessed the virulence potential of one-hundred and six Salmonella strains isolated from food animals and products. A high through-put virulence genes microarray demonstrated Salmonella Pathogenicity Islands (SPI) and adherence genes were highly conserved, while prophages and virulence plasmid genes were variably present. Isolates were grouped by serotype, and virulence plasmids separated S. Typhimurium in two clusters. Atypical microarray results lead to whole genome sequencing (WGS) of S. Infantis Sal147, which identified deletion of thirty-eight SPI-1 genes. Sal147 was unable to invade HeLa cells and showed reduced mortality in Galleria mellonella infection model, in comparison to a SPI-1 harbouring S. Infantis. Microarray and WGS of S. Typhimurium Sal199, established for the first time in S. Typhimurium presence of cdtB and other Typhi-related genes. Characterization of Sal199 showed cdtB genes were upstream of transposase IS911, and co-expressed with other Typhi-related genes. Cell cycle arrest, cytoplasmic distension, and nuclear enlargement were detected in HeLa cells infected by Sal199, but not with S. Typhimurium LT2. Increased mortality of Galleria was detected on infection with Sal199 compared to LT2. Thus, Salmonella isolates were rapidly characterized using a high through-put microarray; helping to identify unusual virulence features which were corroborated by further characterisation. This work demonstrates that the use of suitable screening methods for Salmonella virulence can help assess the potential risk associated with certain Salmonella to humans. Incorporation of such methodology into surveillance could help reduce the risk of emergence of epidemic Salmonella strains.

Salmonella enterica

Prophage

Pathogenicity island

10.1371/journal.pone.0135010

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Citations (44)

Differential diagnosis of multiple sclerosis, is Moyamoya in?

Neurology Psychiatry and Brain Research (2012)

Nuno Mendonça Tiago Parreira Armando Sena Lívia Sousa

Moyamoya Disease

Hemiparesis

Etiology

Neurological examination

10.1016/j.npbr.2012.09.003

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Citations (1)

Molecular diversity of bla genes in Klebsiella pneumoniae and Escherichia coli isolates

Nuno Mendonça

Dissertation presented to obtain a Ph.D. degree in Biology, speciality Microbiology, by Universidade Nova de Lisboa, Faculdade de Ciencias e Tecnologia

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Successful Thrombolysis despite Having an Incidental Unruptured Cerebral Aneurysm

Case Reports in Neurological Medicine (2014)

Diana Gala André Almeida Nadine Monteiro Salgueiro Araújo Ana Paiva Nunes Patrı́cia Ferreira

Purpose . To report a case of successful thrombolysis performed in a patient with an incidental unruptured intracranial aneurysm and review the literature. Case Report . Patient admitted for ischemic stroke due to left posterior cerebral artery occlusion, with an incidental right middle cerebral artery aneurysm, who underwent treatment with tissue plasminogen activator (rtPA) resulting in clinical improvement without complications. Conclusion . The presence of unruptured intracranial aneurysms is considered as a contraindication to thrombolysis, due to a potentially higher hemorrhagic risk of aneurysm rupture. Patients, otherwise, eligible for thrombolysis are usually excluded from receiving this emergent treatment, despite its potential benefits. A reevaluation of the strict exclusion criteria for thrombolysis in acute stroke patients should be considered.

Contraindication

Stroke

Posterior cerebral artery

10.1155/2014/323049

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Citations (6)

Sequence types of Portuguese carbapenem-resistant Acinetobacter baumannii isolates collected over 10 years

Journal of Antimicrobial Chemotherapy (2010)

Gabriela Silva Nuno Mendonça Getulio Teixeira Batista Aida Duarte

11 Toleman M, Karthikeyan K, Sharma M et al. Recent epidemic emergence of blaNDM-1 metallo-b-lactamase in enteric organisms from India is mostly linked to A/C type plasmids. In: Abstracts of the Twentieth European Congress of Clinical Microbiology and Infectious Diseases, Vienna, Austria, 2010. Abstract O-134. European Society of Clinical Microbiology and Infectious Diseases, Basel, Switzerland. J Antimicrob Chemother 2010 doi:10.1093/jac/dkq274 Advance Access publication 22 July 2010

Acinetobacter baumannii

Carbapenem

Sequence (biology)

10.1093/jac/dkq274

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Citations (14)

Association between antimicrobial resistance and virulence inEscherichia coli

Virulence (2012)

Gabriela Silva Nuno Mendonça

Escherichia coli represents a major cause of morbidity and mortality worldwide. The treatment of E. coli infections is now threatened by the emergence of antimicrobial resistance. The dissemination of resistance is associated with genetic mobile elements, such as plasmids, that may also carry virulence determinants. A proficient pathogen should be virulent, resistant to antibiotics, and epidemic. However, the interplay between resistance and virulence is poorly understood. This review aims to critically discuss the association and linked transmission of both resistance and virulence traits in strains from extraintestinal infections in E. coli, and intestinal pathotypes. Despite the numerous controversies on this topic, findings from research published to date indicate that there is a link between resistance and virulence, as illustrated by the successful E. coli ST131 epidemic clone. Perhaps the most commonly accepted view is that resistance to quinolones is linked to a loss of virulence factors. However, the low virulent phylogenetic groups might be more prone to acquire resistance to quinolones. Specific characteristics of the E. coli genome that have yet to be identified may contribute to such genetic linkages. Research based on bacterial populations is sorely needed to help understand the molecular mechanisms underlying the association between resistance and virulence, that, in turn, may help manage the future disseminations of infectious diseases in their entirety.

10.4161/viru.3.1.18382

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Citations (157)

Improved multiplex PCR method for the rapid detection of β-lactamase genes in Escherichia coli of animal origin

Diagnostic Microbiology and Infectious Disease (2006)

Constança Pomba Nuno Mendonça Marta Costa Deolinda Louro Bruno Baptista

Multiplex

10.1016/j.diagmicrobio.2006.03.005

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Citations (22)

Difficult catheter access to the occluded vessel during endovascular treatment of acute ischemic stroke is associated with worse clinical outcome

Journal of NeuroInterventional Surgery (2012)

Marc Ribó Alan Flores Marta Rubiera Jorge Pagola Nuno Mendonça

Background and aim During endovascular procedures for acute ischemic stroke, catheter access to the occluded vessel may be technically difficult or impossible. The aim of this study was to access the impact of difficult catheter access to target the carotid artery on clinical outcome. Methods Anterior circulation stroke patients undergoing transfemoral endovascular procedures where studied. Patients were divided into four groups according to time from groin puncture to target carotid catheterization quartiles. Patients in quartile 4 (Q4) were considered difficult carotid access. We defined several outcome measures: recanalisation (final Thrombolysis in Cerebral Infarction score ≥2a), time from groin puncture to recanalisation and favorable long term outcome (modified Rankin Scale score <3 at 3 months). Results Of 130 patients studied, carotid catheterization was impossible in seven patients (5.1%). These patients had significantly lower rates of recanalization (14.3% vs 80.5%; p<0.01) and favorable outcome (0% vs 36%; p=0.038). Among patients with an accessible carotid artery(n=123), median time from groin puncture to carotid catheterization was 20 min (IQR 10). A negative correlation between time to carotid access and recanalization was observed (r=−0.31; p<0.01). Patients in Q4 (>30 min) had lower rates of recanalization (60.7% vs 82.4%; p=0.02) and a lower favorable outcome (13.6% vs 41.3%; p=0.04). A logistic regression adjusted by age showed that baseline National Institutes of Health Stroke Scale score (OR 0.8; 95% CI: 0.72 to 0.92 p<0.01) and having difficult access (OR 1.3; 95% CI 1.3 to 20.1 p=0.018) independently predicted worse long term outcome. Conclusions Difficult catheter access to target the carotid is common during acute endovascular treatment of stroke patients and is associated with a worse clinical outcome. If transfemoral access appears difficult, alternative access such as direct carotid puncture could be explored.

Stroke

Solitaire Cryptographic Algorithm

10.1136/neurintsurg-2012-010438

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Citations (143)

Communicating Science Matters: Evidence from the BASCOE Study, a quantitative study from Brain Awareness Week outreach activities

Carlos Pita Inês P. Silva Ana Rita Colaço Margarida Brotas Beatriz Felgueiras

While the importance of science communication and outreach is widely acknowledged, their impact is often overlooked or not measured. To address this question we carried a study aimed to quantitatively assess the influence of science communication and outreach activities on knowledge acquisition and retention by secondary school students during Brain Awareness Week (BAW) 2023 in Lisbon, Portugal from a population of 428 students raging from 6th to 12th grade (6-12 years of formal education). Analysis of pre- and post-event questionnaire data revealed a significant improvement in participant knowledge scores seven days post-activity compared to the baseline. These findings highlight the importance of such quantitative evaluations and underscore the pivotal role of science communication in enhancing scientific knowledge in schoolchildren.

Outreach

Baseline (sea)

Risk Communication

Formal education

10.31235/osf.io/ebr2k

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Citations (0)

Occurrence of a novel SHV-type enzyme (SHV-55) among isolates of Klebsiella pneumoniae from Portuguese origin in a comparison study for extended-spectrum β-lactamase–producing evaluation

Diagnostic Microbiology and Infectious Disease (2006)

Nuno Mendonça Eugénia Ferreira Manuela Caniça

Broth microdilution

Agar dilution