Jielin Chen

Nanjing University

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Co-Authors

Jun Zhou

Nanjing University

Huangxian Ju

Nanjing University

Mingpan Cheng

China Pharmaceutical University

Jean‐Louis Mergny

Czech Academy of Sciences, Institute of Biophysics

David Monchaud

Institut de Chimie

Chunhai Fan

Renji Hospital

Dehui Qiu

Nanjing University

Yuzhe Huang

Kyungsung University

JŠ

Jiŕı́ Šponer

Regional Centre of Advanced Technologies and Materials

Petr Stadlbauer

Czech Academy of Sciences, Institute of Biophysics

Cooperative Institutions

Shanghai Jiao Tong University

Central South University

Chinese Academy of Sciences

Xiangya Hospital Central South University

Nanjing University

Chinese Academy of Medical Sciences & Peking Union Medical College

Zhejiang University

Anhui University

University of Chinese Academy of Sciences

Shanghai Institute of Applied Physics

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Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field

Saudi Pharmaceutical Journal (2017)

Jianghong Huang Daming Wang Jielin Chen Wei Liu Li Duan

This study was conducted to investigate the effect of magnetic nanoparticle composite scaffold under a pulsed electromagnetic field on bone marrow mesenchymal stem cells (BMSCs), which was achieved by examining the biological behaviors of cell adhesion, proliferation and differentiation on the surface of the scaffolds. This may provide some experimental evidence for the use of magnetic nanoparticles in medical application. The magnetic nanoparticle composite scaffolds were evaluated and characterized by the following indexes: the cell proliferation was detected by the CCK-8 method, the alkaline phosphatase (ALP) activity was examined by a detection kit, and the expression of type I collagen and osteocalcin gene were evaluated by RT-PCR. The CCK-8 test showed that there was no significant difference in Group A (BMSCs-seeded magnetic scaffolds under the electromagnetic field), B (BMSCs-seeded magnetic scaffolds) and C (BMSCs cultured alone) (P > 0.05). The value for the ALP activity in Group A was higher than the other two groups. In addition, the RT-PCR results showed that the expression of type I collagen gene in Group A was enhanced (P < 0.05), suggesting that the magnetic nanoparticles combined with the pulsed electromagnetic field had a positive effect on the osteogenic differentiation of BMSCs. However, the expression of osteocalcin was not significantly different in three groups (P > 0.05). To conclude, magnetic nanoparticles may induce the osteogenic differentiation with the action of the pulsed electromagnetic field.

10.1016/j.jsps.2017.04.026

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Citations (30)

Genetic Circuits and Chemotaxis Induced Bacterial Cloning on Media Plate

Research and Reviews: Journal of Microbiology and Biotechnology (2016)

Wei Jiang Chun Tang Jielin Chen Yiying Lei Ruihua Zhang

Objective: Synthetic biology demonstrates its broad application perspective in the fields of medicine, chemical synthesis, and the production of energy. Methods: The character that E. coli responding to the stimulus is named as chemo taxis which has widely applications such as measurement efficiency of RBS and promoter, suicide mechanism, oscillation timer etc. Results: A circuit to control the motility of E. coli (run or tumble) and form the patterns such as conic curves was constructed. The strength of the promoter and the efficiency of RBS were successfully characterized by using the circuit and chemo taxis that can be used to characterize most of the promoters, the RBS efficiency, terminator efficiency and expression strength of target genes etc. A new suicide mechanism, utilizing the hyperosmotic pressure, was built to induce the growth of E. coli Pattern model is the fundamental force in the coordination of multicellular behavior in the bacterial community or a large complex system. Conclusion: The sources of stress (such as sodium chloride and sucrose) be to generate hypertonic very cheap, convenient and environmentally friendly while antibiotics are expensive and have a bad effect on the environment because of drug-resistant microorganisms.

Multicellular organism

Synthetic Biology

Stimulus (psychology)

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Caractéristiques écologiques de l'acarien des agrumes phyllocoptruta oleivora (Ashm.)

Li Lung-Shu Huang Fang-Neng Jielin Chen

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Effects of Sulforaphane on Human Chondrocytes Cultured <I>In Vitro</I>

Journal of Biomaterials and Tissue Engineering (2016)

Weimin Zhu Jielin Chen Jiaming Cui Jianghong Huang Jianyi Xiong

Sulforaphane

10.1166/jbt.2016.1552

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Citations (1)

GPR162 activates STING dependent DNA damage pathway as a novel tumor suppressor and radiation sensitizer

Signal Transduction and Targeted Therapy (2023)

Yao Long Jiaxing Guo Jielin Chen Jingyue Sun Haiyan Wang

In the treatment of most malignancies, radiotherapy plays a significant role. However, the resistance of cancer cells to ionizing radiation (IR) is the main reason for the failure of radiotherapy, which causes tumor recurrence and metastasis. In this study, we confirmed that GPR162, an orphan receptor in the G-protein-coupled receptor family, acted as a novel radiotherapy sensitizer by interacting with the stimulator of interferon genes (STING), which targeted DNA damage responses, activated IRF3, accelerated the activation of type I interferon system, promoted the expression of chemokines including CXCL10 and CXCL4, and inhibited the occurrence and development of tumors. Interestingly, the activation of STING by overexpression of GPR162 was independent of the classical pathway of cGAS. STING inhibitors could resist the antitumor effect of overexpression of GPR162 in IR-induced mouse models. In addition, most solid tumors showed low expression of GPR162. And the higher expression of GPR162 indicated a better prognosis in patients with lung adenocarcinoma, liver cancer, breast cancer, etc. In summary, these results suggested that GPR162 may serve as a potential sensitizer of radiotherapy by promoting radiotherapy-induced STING-IFN production and increasing the expression of chemokines including CXCL10 and CXCL4 in DNA damage response, providing an alternative strategy for improving cancer radiotherapy.

Sting

10.1038/s41392-022-01224-3

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Citations (19)

Deposition of DNA Nanostructures on Highly Oriented Pyrolytic Graphite

Advanced Materials Interfaces (2024)

Yanjing Gao Jielin Chen Jiangbo Liu Mingqiang Li Yichun Wang

Abstract The integration of the addressability of DNA nanostructures and the electronic sensitivity of sp 2 carbon‐based materials presents a promising avenue for the development of powerful sensitive biodevices for detecting biological signals and substances with high sensitivity. Essential to this prospect is the attainment of high‐shape fidelity immobilization of DNA nanostructures on these sp 2 carbon substrates. However, it is challenging due to the stability of DNA nanostructures on these substrates. In this study, the adsorption behavior of DNA nanostructures is explored on a highly oriented pyrolytic graphite (HOPG) surface. The observations revealed a severe structural destabilization of DNA nanostructures upon interacting with bare HOPG. Immobilization of DNA nanostructures with high shape‐fidelity can be achieved by modifying the surface property of HOPG and enhancing the rigidity of DNA nanostructures. In addition, DNA tetrahedron origami is employed as a rigid, precise, and programmable three‐dimensional framework for controlled patterning of gold nanoparticles on HOPG surface, demonstrating the potential for constructing plasmonic devices and arranging other functional species on sp 2 carbon‐based substrates for specific applications. This study shows the potential for customizable functionalization of sp 2 carbon‐based substrates and tailored design of biodevices at the interface through integration with DNA nanostructures.

Pyrolytic carbon

Carbon fibers

Surface Modification

10.1002/admi.202400557

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DNA Framework-Engineered Assembly of Cyanine Dyes for Structural Identification of Nucleic Acids

JACS Au (2024)

Cong Li Jielin Chen Tiantian Man Bin Chen Jiang Li

DNA nanostructures serve as precise templates for organizing organic dyes, enabling the creation of programmable artificial photonic systems with efficient light-harvesting and energy transfer capabilities. However, regulating the organization of organic dyes on DNA frameworks remains a great challenge. In this study, we investigated the factors influencing the self-assembly behavior of cyanine dye K21 on DNA frameworks. We observed that K21 exhibited diverse assembly modes, including monomers, H-aggregates, J-aggregates, and excimers, when combined with DNA frameworks. By manipulating conditions such as the ion concentration, dye concentration, and structure of DNA frameworks, we successfully achieved precise control over the assembly modes of K21. Leveraging K21's microenvironment-sensitive fluorescence properties on DNA nanostructures, we successfully discriminated between the chirality and topology structures of physiologically relevant G-quadruplexes. This study provides valuable insights into the factors influencing the dynamic assembly behavior of organic dyes on DNA framework nanostructures, offering new perspectives for constructing functional supramolecular aggregates and identifying DNA secondary structures.

Cyanine

DNA origami

Template

DNA nanotechnology

10.1021/jacsau.3c00826

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Citations (6)

Author Correction: GPR162 activates STING dependent DNA damage pathway as a novel tumor suppressor and radiation sensitizer

Signal Transduction and Targeted Therapy (2023)

Yao Long Jiaxing Guo Jielin Chen Jingyue Sun Haiyan Wang

Sting

10.1038/s41392-023-01508-2