Jiaxin Pi

Tianjin University of Traditional Chinese Medicine

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Zhidong Liu

Tianjin University of Traditional Chinese Medicine

Dongli Qi

Shenyang Ligong University

Pan Guo

Tianjin University of Traditional Chinese Medicine

Nan Li

Army Medical University

Dereje Kebebe

Jimma University

Ying Zhang

Tianjin University of Traditional Chinese Medicine

Yumei Wu

Zunyi Medical University

Shuya Wang

South College

Peng Lü

Qiqihar Medical University

Xutong Ma

Tianjin University of Traditional Chinese Medicine

Cooperative Institutions

Tianjin University of Traditional Chinese Medicine

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Tianjin haihe hospital

Harbin Medical University

Nanjing Medical University

Peking University

University of Chinese Academy of Sciences

Capital Medical University

Sun Yat-sen University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Study of penetration mechanism of labrasol on rabbit cornea by Ussing chamber, RT-PCR assay, Western blot and immunohistochemistry

Asian Journal of Pharmaceutical Sciences (2018)

Pan Guo Nan Li Lili Fan Jun Lu Boying Liu

Labrasol, as a non-ionic surfactant, can enhance the permeation and absorption of drugs, and is extensively used in topical, transdermal, and oral pharmaceutical preparations as an emulsifier and absorption enhancer. Recent studies in our laboratory have indicated that labrasol has a strong absorption enhancing effect on different types of drugs in vitro and in vivo. This study was performed to further elucidate the action mechanism of labrasol on the corneal penetration. In this research, the fluorescein sodium, a marker of passive paracellular transport of tight junction, was selected as the model drug to assess the effect of labrasol on in vitro corneal permeability. To investigate the continuous and real-time influence of labrasol on the membrane permeability and integrity, the Ussing chamber system was applied to monitor the electrophysiological parameters. And, furthermore, we elucidated the effect of labrasol on excised cornea at the molecular level by application of RT-PCR, Western blot, and immunohistochemical staining. The results indicated that labrasol obviously enhance the transcorneal permeability of fluorescein sodium, and the enhancement was realized by interacting with and down-regulating the associated proteins, such as F-actin, claudin-1 and β-catenin, which were contributed to cell-cell connections, respectively.

Ussing chamber

Paracellular transport

Penetration (warfare)

10.1016/j.ajps.2018.05.005

Cite

Citations (14)

In vitro skin retention and drug permeation study of Tongluo-Qutong rubber plaster by UPLC/UV/MS/MS

Figshare (2022)

Ying Zhang Jiaxin Pi Wen Li Ludan Zhang Xutong Ma

Abstract Tongluo-Qutong rubber plaster (TQRP), a typical Chinese patent medicine that contains 13 different herbal remedies, is widely used in clinical practice for the treatment of cervical spondylosis and osteoarthritis. However, due to a lack of in vitro transdermal studies, the active ingredients of TQRP have not been fully elucidated. This presents a huge obstacle for quality evaluation, pharmacokinetic studies and clinical safety assessment of TQRP. In this work, a UPLC/UV/MS/MS method was established and validated to evaluate five analytes in TQRP. The validation demonstrated linearity (r > 0.99), specificity (no co-eluting peaks at the retention times of the analytes), and precision (RSD < 15%) within acceptable parameters. A skin permeation study was performed to determine the concentrations of drugs delivered to the dermis. The 24-hour cumulative permeation of ferulic acid, aleo-emodin, emodin and piperine were 303.68, 709.31, 671.06 and 25561.01 ng/cm2, respectively. According to the fitting data of the TQRP active components, skin permeation was mainly due to a combination of passive diffusion and drug release after matrix erosion.

10.6084/m9.figshare.19884224

Cite

Citations (0)

A nano-cocrystal strategy to improve the dissolution rate and oral bioavailability of baicalein

Asian Journal of Pharmaceutical Sciences (2018)

Jiaxin Pi Shuya Wang Li Wen Dereje Kebebe Ying Zhang

Baicalein (BE) is one of the main active flavonoids representing the variety of pharmacological effects including anticancer, anti-inflammatory and cardiovascular protective activities, but it's very low solubility, dissolution rate and poor oral absorption limit the therapeutic applications. In this work, a nano-cocrystal strategy was successfully applied to improve the dissolution rate and bioavailability of BE. Baicalein-nicotinamide (BE-NCT) nano-cocrystals were prepared by high pressure homogenization and evaluated both in vitro and in vivo. Physical characterization results including scanning electron microscopy, dynamic light scattering, powder X-ray diffraction and differential scanning calorimetry demonstrated that BE-NCT nano-cocrystals were changed into amorphous state with mean particle size of 251.53 nm. In the dissolution test, the BE-NCT nano-cocrystals performed 2.17-fold and 2.54-fold enhancement than BE coarse powder in FaSSIF-V2 and FaSSGF. Upon oral administration, the integrated AUC0 − t of BE-NCT nano-cocrystals (6.02-fold) was significantly higher than BE coarse powder (1-fold), BE-NCT cocrystals (2.87-fold) and BE nanocrystals (3.32-fold). Compared with BE coarse powder, BE-NCT cocrystals and BE nanocrystals, BE-NCT nano-cocrystals possessed excellent performance both in vitro and in vivo evaluations. Thus, it can be seen that nano-cocrystal is an appropriate novel strategy for improving dissolution rate and bioavailability of poor soluble natural products such as BE.

Cocrystal

Baicalein

Powder Diffraction

10.1016/j.ajps.2018.04.009

Cite

Citations (75)

Self-micro emulsifying formulation improved intestinal absorption and oral bioavailability of bakuchiol

Archives of Pharmacal Research (2014)

Jiaxin Pi Xu Gao Yue Yu Zheng Yin Zhuangzhi Zhu

Psoralea corylifolia

Jejunum

Zeta potential

10.1007/s12272-014-0499-x

Cite

Citations (4)

Salvianolic acid B protects against myocardial damage caused by nanocarrier TiO<sub>2</sub>; and synergistic anti-breast carcinoma effect with curcumin via codelivery system of folic acid-targeted and polyethylene glycol-modified TiO<sub>2</sub> nanoparticles

International Journal of Nanomedicine (2016)

Lingling Ding Jiawei Li Rui Huang Zhidong Liu Chunhua Li

Abstract: Targeted delivery by the folate ligand is an effective way to enhance an anti-breast carcinoma effect, due to its high affinity for the folate receptor, which is overexpressed in many tumor cells. In this study, we firstly synthesized a folic acid (FA)-targeted and polyethylene glycol (PEG)-modified TiO 2 nanocarrier. Then, an FA-PEG-TiO 2 nanoparticle (NP) codelivery system loaded with curcumin and salvianolic acid B were prepared by emulsion evaporation–solidification at low temperature. The obtained folate-targeted NPs (FA-NPs) showed more cytotoxicity on MCF7 cells and MDA-MB-231 cells than a nontargeted NP group. Apart from a synergistic anti-breast cancer effect with curcumin, salvianolic acid B protects the cardiovascular system from oxidative injury by the TiO 2 nanocarrier. With coumarin 6 as a fluorescent probe to observe cellular uptake of NPs, the results of in vitro cellular uptake demonstrated FA-NPs exhibited higher cellular uptake and accumulation in MCF7 cells and MDA-MB-231 cells than nontargeted NPs. Then, in vivo biodistribution of NPs was further qualitatively and quantitatively confirmed by in vivo imaging. More importantly, the animal study further suggested that FA-NPs had significantly stronger antitumor effects via receptor-mediated targeted delivery. Consequently, FA-PEG-TiO 2 NPs loaded with curcumin and salvianolic acid B could be a promising drug-delivery system to treat breast cancer. Keywords: breast cancer, codelivery, curcumin, FA-PEG-TiO 2 , nanoparticles, salvianolic acid B

Nanocarriers

Folate receptor

Biodistribution

10.2147/ijn.s107767

Cite

Citations (30)

The Nanostructured lipid carrier gel of Oroxylin A reduced UV-induced skin oxidative stress damage

Colloids and Surfaces B Biointerfaces (2022)

Shan Zhu Zhiyue Zhao Wenxiao Qin Tao Liu Yi Yang

Photoaging

Skin Aging

Barrier function

10.1016/j.colsurfb.2022.112578

Cite

Citations (19)

Brain targeting of Baicalin and Salvianolic acid B combination by OX26 functionalized nanostructured lipid carriers

International Journal of Pharmaceutics (2019)

Yumei Wu Xunan Song Dereje Kebebe Xinyue Li Zhifeng Xue

Microdialysis

10.1016/j.ijpharm.2019.118754

Cite

Citations (29)

[Study on prescription of self-microemulsifying drug delivery system of mangiferin phospholipid complex].

PubMed (2012)

Xiao-yu Xuan Yajing Wang Hui Tian Jiaxin Pi Sun Shi-zhen

To study formulation of self-microemulsifying drug delivery system (SMEDDS) of mangiferin phospholipid complex and improve dissolution and bioavailability of mangiferin.Ternary phase diagram was applied to optimize the prescription of self-microemulsifying drug delivery system of mangiferin phospholipid complex, and the best recipe was selected by comprehensive evaluation of the speed of microemulsifying, microemulsion size and electric potential.The optimum formulation of SMEDDS was composed of IPM-Cremphor EL35-labrasol = 2 : 4.8 : 3.2.Self-microemulsifying drug delivery system of mangiferin phospholipid complex can effectively improve the dissolution of Mangiferin.

Mangiferin

Microemulsion

Source

Cite

Citations (9)

Preparation Procedure and Pharmacokinetic Study of Water-in-Oil Nanoemulsion of Panax Notoginseng Saponins for Improving the Oral Bioavailability

Current Drug Delivery (2016)

Zhidong Liu Qian Zhang Lingling Ding Chunhua Li Zhongpeng Yin

Due to the effects of gastric acid, glycosidase and intestinal flora in the gastrointestinal environment, panax notoginseng saponins (PNS) can easily be resolved and metabolized when it is administered orally, limiting its oral bioavailability.The formula of PNS nanoemulsion (PNS-N) was optimized using a pseudoternary phase diagram, and the PNS-N was prepared by high pressure homogenization. The type, particle size, polydispersity index (PDI), refractive index, pH and content of PNS-N were characterized. In vitro characteristics were investigated by drug release and physical stability. The pharmacokinetic properties of PNS-N were studied with rat intestine and SD rats. The optimized nanoemulsion formulation was Labrafil M 1944CS (58%), SP/EtOH (Km=1) (25%), solution of PNS (400mg/ml) (17%).The results showed that the average particle size was (28.17±0.39) nm with PDI of 0.116±0.032, refractive index of 1.4491±0.0009 and pH of 4.58±0.03. In addition, the contents of R1, Rg1 and Rb1 were (4.64±0.21) mg/mL, (19.16±0.27) mg/mL and (11.77±0.08) mg/mL, respectively. The optimized PNS-N formulation exhibited a sustained drug release with good stability. PNS-N is still clear and transparent, without layering and precipitation after six months. In the study of absorption kinetics of PNS-N in rat intestine, the Papp of three main components of PNS-N increased 5 times than PNS solution (PNS-SOL) in rat intestine. And pharmacokinetic study in SD rats suggested a 2.58-fold increase of oral bioavailability compared with PNS-SOL.The PNS-N has increased the absolute availability of PNS obviously and nanoemulsion is a potential formulation to improve oral bioavailability for PNS.

Panax notoginseng

Poloxamer 407

10.2174/1567201812666150608095517

Cite

Citations (16)

Preparation, optimization and cellular uptake study of tanshinone I nanoemulsion modified with lactoferrin for brain drug delivery

Pharmaceutical Development and Technology (2019)

Yumei Wu Bing Zhang Dereje Kebebe Lili Guo Hong Guo

Tanshinone I (TSI) is one of the bioactive compound obtained from the root of Salvia miltiorrhiza which is a well-known traditional Chinese medicine (TCM) used for the treatment of various diseases. Although TSI possesses several pharmacological effects, it has poor water solubility, blood-brain barrier (BBB) permeability and brain bioavailability. Therefore, in the present study, we developed TSI nanoemulsion (TSI-NE) modified with a brain targeting ligand (Lactoferrin (Lf)) to improve the BBB permeability. Pseudo-ternary phase diagrams were used to optimize the formulation. The optimal TSI-NE and TSI-Lf-NE were prepared and characterized. Finally, the uptake of TSI-Lf-NE by mouse brain microvascular endothelial cell line (bEnd.3 cells) was assessed using Coumarin-6 as a fluorescent probe. The results of the study showed that the stable optimal formulation of O/W nanoemulsion was successfully developed and modified with Lf. The cellular uptake study has shown that the fluorescence intensity (FI) increased with time over the incubation period. The FI at all time intervals increased in the following order: Coumarin-6-Solution＜Coumarin-6-NE＜Coumarin-6-Lf-NE. The results suggest that the BBB permeability of Coumarin-6-Lf-NE was better than those of Coumarin-6-NE and Coumarin-6 solution. Lf modified nanoemulsion has great potential for improving the brain delivery of TSI.

Salvia miltiorrhiza

Lactoferrin

10.1080/10837450.2019.1621897

Cite

Citations (24)