In coronavirus disease 2019 (COVID-19) patients, interleukin (IL)-6 is one of the leading factors causing death through cytokine release syndrome. Hence, identification of IL-6 downstream from clinical patients' transcriptome is very valid for analyses of its mechanism. However, clinical study is conditional and time consuming to collect optional size of samples, as patients have the clinical heterogeneity. A possible solution is to deeply mine the relative existing data. Several transcriptome-based studies on other diseases or treatments have revealed different genes to be regulated by IL-6. Through our meta-analysis of these transcriptome datasets, 352 genes were suggested to be regulated by IL-6 in different biological conditions, some of which were related to virus infection and cardiovascular disease. Among them, 232 genes were not identified by current transcriptome studies from clinical research. ICAM1 and PFKFB3 were the most significantly upregulated genes in our meta-analysis and could be employed as biomarkers in patients with severe COVID-19. In general, a meta-analysis of transcriptome datasets could be an alternative way to analyze the immune response and complications of patients suffering from severe COVID-19 and other emergency diseases.
Objective:To analyze the distribution of pathogens in respiratory tract and their drug resistance in the patients with acute exacerbation chronic obstructive pulmonary disease(AE-COPD) for a better use of antibiotics in treatment.Methods: The sputum samples of 193 AE-COPD patients were collected from our hospital and the pathogens species in all samples were tested.The drug sensitivity were determined by K-B paper method.Results: 158 strains of pathogens were found in all sputum samples,of which 67.3% were Gram-negtive,20.0% Gram-positive.Among the Gram-negtive bacillis,the main pathogens included Pseudomonas(32.8%),Moraxelle catarrhalis(21.2%),Klebsiella Pneumonia(19.5%).Among the Gram-positive coccis,the main pathogens included Staphyloccus aureus(7.1%),streptococcus pneumoniae(5.0%),and Staphylococcus epidermidis(3.7%).The results of drug sensitivity test showed that Pseudomonas were more sensitive to meropenem and imipenem,Moraxelle catarrhalis also were more sensitive to meropenem and imipenem,and that most of the Gram-positive coccis were more sensitive to amikacin and vancomycin.Conclusions: Gram-negative bacillus are predominant in lower respiratory tract in AE-COPD patients with higher frequency of drug resistance.Antibiotics collected for AE-COPD must be according to the susceptibility test results.
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