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Lourdes Mateos‐Hernández

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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Alejandro Cabezas‐Cruz

Bipar

José de la Fuente

Consejo Superior de Investigaciones Científicas

Dasiel Obregón

University of Guelph

Margarita Villar

Instituto de Investigación en Recursos Cinegéticos

Alejandra Wu‐Chuang

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Apolline Maître

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Ladislav Šimo

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Sara Moutailler

Bipar

Pilar Alberdi

Instituto de Investigación en Recursos Cinegéticos

Lianet Abuin‐Denis

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Cooperative Institutions

Instituto de Salud Carlos III

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Consejo Superior de Investigaciones Científicas

Bipar

École Nationale Vétérinaire d'Alfort

University of Castilla-La Mancha

Instituto de Investigación en Recursos Cinegéticos

Centro de Ingeniería Genética y Biotecnología

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Centro Nacional de Microbiologia

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Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment

BMC Microbiology (2024)

Myriam Kratou Apolline Maître Lianet Abuin‐Denis Elianne Piloto‐Sardiñas Ivan Corona-Guerrero

Haemaphysalis longicornis

Haemaphysalis

10.1186/s12866-024-03468-1

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Detection and subtyping of dengue 1–4 and yellow fever viruses by means of a multiplex RT‐nested‐PCR using degenerated primers

Tropical Medicine & International Health (2006)

María Paz Sánchez‐Seco D. Rosario Lourdes Mateos‐Hernández Cristina Domingo Katia Valdés

Differential diagnosis of infections that cause similar diseases and may be active simultaneously in the same geographical areas is greatly needed. Dengue and yellow fever viruses (DENV and YFV) are transmitted by the same species of mosquito and both can cause haemorrhagic fever symptoms. These viruses are active mainly in regions where expensive and sophisticated technologies are not available. Our objective was to develop a simple, reliable and easy-to-perform method to detect and identify these viruses.We slightly modified a generic RT-PCR able to detect the mentioned viruses and other members of this genus: specific primers for each one of these viruses were designed and included in the nested reaction instead of one of the generic ones. The reaction was optimized and viruses are amplified giving rise to bands of different sizes distinguishable in agarose gels.This test is able to detect and identify the four DENVs and YFV to a high level of sensitivity and specificity and can be used with clinical samples. This simple, reliable and easy-to-perform method able to detect and identify dengue 1-4 and YFV can be used in poor endemic countries.

Subtyping

Multiplex

10.1111/j.1365-3156.2006.01696.x

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Generic RT-nested-PCR for detection of flaviviruses using degenerated primers and internal control followed by sequencing for specific identification

Journal of Virological Methods (2005)

María Paz Sánchez‐Seco D. Rosario Cristina Domingo Lourdes Mateos‐Hernández Katia Valdés

West Nile virus

Identification

Flavivirus

10.1016/j.jviromet.2005.01.025

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Cholinomimetics-mediated salivary gland secretion in hard ticks - how does it actually work?

Ladislav Šimo Lourdes Mateos‐Hernández Cáinà Nìng Sabine Rakotobé Alejandro Cabezas Cruz

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A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Journal of Visualized Experiments (2020)

Lourdes Mateos‐Hernández Sabine Rakotobé Baptiste Defaye Alejandro Cabezas‐Cruz Ladislav Šimo

Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of mouse models in tick research is critical for understanding their biology and tick-host-pathogen interactions. Here we demonstrate a non-laborious technique for the feeding of immature stages of hard ticks on laboratory mice. The benefit of the method is its simplicity, short duration, and the ability to monitor or collect ticks at different time points of an experiment. In addition, the technique allows attachment of two individual capsules on the same mouse, which is beneficial for a variety of experiments where two different groups of ticks are required to feed on the same animal. The non-irritating and flexible capsule is made from easily accessible materials and minimizes the discomfort of the experimental animals. Furthermore, euthanasia is not necessary, mice recover completely after the experiment and are available for re-use.

Rodent model

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Tick–human interactions: from allergic klendusity to the α-Gal syndrome

Biochemical Journal (2021)

Alejandro Cabezas‐Cruz Adnan Hodžić Lourdes Mateos‐Hernández Marinela Contreras José de la Fuente

Ticks and the pathogens they transmit, including bacteria, viruses, protozoa, and helminths, constitute a growing burden for human and animal health worldwide. The ability of some animal species to acquire resistance to blood-feeding by ticks after a single or repeated infestation is known as acquired tick resistance (ATR). This resistance has been associated to tick-specific IgE response, the generation of skin-resident memory CD4+ T cells, basophil recruitment, histamine release, and epidermal hyperplasia. ATR has also been associated with protection to tick-borne tularemia through allergic klendusity, a disease-escaping ability produced by the development of hypersensitivity to an allergen. In addition to pathogen transmission, tick infestation in humans is associated with the α-Gal syndrome (AGS), a type of allergy characterized by an IgE response against the carbohydrate Galα1-3Gal (α-Gal). This glycan is present in tick salivary proteins and on the surface of tick-borne pathogens such as Borrelia burgdorferi and Anaplasma phagocytophilum, the causative agents of Lyme disease and granulocytic anaplasmosis. Most α-Gal-sensitized individuals develop IgE specific against this glycan, but only a small fraction develop the AGS. This review summarizes our current understanding of ATR and its impact on the continuum α-Gal sensitization, allergy, and the AGS. We propose that the α-Gal-specific IgE response in humans is an evolutionary adaptation associated with ATR and allergic klendusity with the trade-off of developing AGS.

Anaplasma phagocytophilum

Tick infestation

Tick-borne disease

Ehrlichiosis

10.1042/bcj20200915

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Differential nested patterns of Anaplasma marginale and Coxiella-like endosymbiont across Rhipicephalus microplus ontogeny

Microbiological Research (2024)

Lianet Abuin‐Denis Elianne Piloto‐Sardiñas Apolline Maître Alejandra Wu‐Chuang Lourdes Mateos‐Hernández

Understanding the intricate ecological interactions within the microbiome of arthropod vectors is crucial for elucidating disease transmission dynamics and developing effective control strategies. In this study, we investigated the ecological roles of Coxiella-like endosymbiont (CLE) and Anaplasma marginale across larval, nymphal, and adult stages of Rhipicephalus microplus. We hypothesized that CLE would show a stable, nested pattern reflecting co-evolution with the tick host, while A. marginale would exhibit a more dynamic, non-nested pattern influenced by environmental factors and host immune responses. Our findings revealed a stable, nested pattern characteristic of co-evolutionary mutualism for CLE, occurring in all developmental stages of the tick. Conversely, A. marginale exhibited variable occurrence but exerted significant influence on microbial community structure, challenging our initial hypotheses of its non-nested dynamics. Furthermore, in silico removal of both microbes from the co-occurrence networks altered network topology, underscoring their central roles in the R. microplus microbiome. Notably, competitive interactions between CLE and A. marginale were observed in nymphal network, potentially reflecting the impact of CLE on the pathogen transstadial-transmission. These findings shed light on the complex ecological dynamics within tick microbiomes and have implications for disease management strategies.

Rhipicephalus microplus

10.1016/j.micres.2024.127790

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Citations (1)

Differential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR

Acta Tropica (2022)

Pavle Banović Elianne Piloto‐Sardiñas Dragana Mijatović Angélique Foucault‐Simonin Verica Simin

Anaplasma phagocytophilum

Borrelia afzelii

Bartonella

Ixodes scapularis

Anaplasmosis

Eschar

10.1016/j.actatropica.2022.106756

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Heat‐inactivated Mycobacterium bovis protects zebrafish against mycobacteriosis

Journal of Fish Diseases (2018)

Vladimir López María Á. Risalde Marinela Contreras Lourdes Mateos‐Hernández Joaquín Vicente

Control of mycobacterial infection constitutes a priority for human and animal health worldwide. However, effective vaccines are needed for the control of human and animal tuberculosis (TB). Adult zebrafish have become a useful model for studying the pathophysiology of mycobacterial infection and for the development of novel interventions for TB control and prevention. Recently, parenteral and oral immunization with the heat-inactivated Mycobacterium bovis vaccine (M. bovis IV) protected wild boar against TB. The objectives of this study were to provide additional support for the role of M. bovis IV in TB control using the zebrafish model and to conduct the first trial with this vaccine for the control of fish mycobacteriosis. The results showed that M. bovis IV protected zebrafish against mycobacteriosis caused by low and high infection doses of Mycobacterium marinum and provided evidence suggesting that the protective mechanism elicited by M. bovis IV in zebrafish as in other species is based on the activation of the innate immune response through the C3 pathway, with a role for the regulatory protein Akr2 in this process. These results encourage the use of M. bovis IV for TB control in different species.

Mycobacterium marinum

Tuberculosis vaccines

10.1111/jfd.12847

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Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus

Microbiome (2023)

Alejandra Wu‐Chuang Lourdes Mateos‐Hernández Apolline Maître Ryan O. M. Rego Radek Šíma

Abstract Background Ticks can transmit a broad variety of pathogens of medical importance, including Borrelia afzelii , the causative agent of Lyme borreliosis in Europe. Tick microbiota is an important factor modulating, not only vector physiology, but also the vector competence. Anti-microbiota vaccines targeting keystone taxa of tick microbiota can alter tick feeding and modulate the taxonomic and functional profiles of bacterial communities in the vector. However, the impact of anti-microbiota vaccine on tick-borne pathogen development within the vector has not been tested. Results Here, we characterized the Ixodes ricinus microbiota modulation in response to B. afzelii infection and found that the pathogen induces changes in the microbiota composition, its beta diversity and structure of bacterial community assembly. Tick microbiota perturbation by anti-microbiota antibodies or addition of novel commensal bacteria into tick midguts causes departures from the B. afzelii -induced modulation of tick microbiota which resulted in a lower load of the pathogen in I. ricinus . Co-occurrence networks allowed the identification of emergent properties of the bacterial communities which better defined the Borrelia infection-refractory states of the tick microbiota. Conclusions These findings suggest that Borrelia is highly sensitive to tick microbiota perturbations and that departure from the modulation induced by the pathogen in the vector microbiota pose a high cost to the spirochete. Network analysis emerges as a suitable tool to identify emergent properties of the vector microbiota associated with infection-refractory states. Anti-microbiota vaccines can be used as a tool for microbiota perturbation and control of important vector-borne pathogens.

Borrelia afzelii

Medical microbiology

10.1186/s40168-023-01599-7

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Citations (17)

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