M. Peiteado

Instituto de Cerámica y Vidrio

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A. C. Caballero

Instituto de Cerámica y Vidrio

Teresa Jardiel

Instituto de Cerámica y Vidrio

J.F. Fernández

Instituto de Cerámica y Vidrio

Juan Carlos Gómez Martı́n

Instituto de Astrofísica de Andalucía

Olga Muñoz

Instituto de Astrofísica de Andalucía

David G. Calatayud

Universidad Autónoma de Madrid

Julia Martikainen

Instituto de Astrofísica de Andalucía

Mara S. Bernardo

Instituto de Cerámica y Vidrio

M. Villegas

EIA University

J. de Frutos

Universidad Politécnica de Madrid

Cooperative Institutions

Consejo Superior de Investigaciones Científicas

Instituto de Cerámica y Vidrio

Universidad Complutense de Madrid

Universidad Autónoma de Madrid

Centre National de la Recherche Scientifique

Jožef Stefan Institute

Universidad Politécnica de Madrid

Instituto de Ciencia de Materiales de Madrid

Instituto de Astrofísica de Andalucía

University of Helsinki

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Hybrid Hierarchical Heterostructures of Nanoceramic Phosphors as Imaging Agents for Multiplexing and Living Cancer Cells Translocation

ACS Applied Bio Materials (2021)

David G. Calatayud Teresa Jardiel Mara S. Bernardo Vincenzo Mirabello Haobo Ge

Existing fluorescent labels used in life sciences are based on organic compounds with limited lifetime or on quantum dots which are either expensive or toxic and have low kinetic stability in biological environments. To address these challenges, luminescent nanomaterials have been conceived as hierarchical, core–shell structures with spherical morphology and highly controlled dimensions. These tailor-made nanophosphors incorporate Ln:YVO4 nanoparticles (Ln = Eu(III) and Er(III)) as 50 nm cores and display intense and narrow emission maxima centered at ∼565 nm. These cores can be encapsulated in silica shells with highly controlled dimensions as well as functionalized with chitosan or PEG5000 to reduce nonspecific interactions with biomolecules in living cells. Confocal fluorescence microscopy in living prostate cancer cells confirmed the potential of these platforms to overcome the disadvantages of commercial fluorophores and their feasibility as labels for multiplexing, biosensing, and imaging in life science assays.

Nanomaterials

Biomolecule

Fluorescence-lifetime imaging microscopy

Biological Imaging

10.1021/acsabm.0c01417

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Citations (7)

Processing strategies to control grain growth in ZnO based varistors

Journal of the European Ceramic Society (2005)

M. Peiteado J.F. Fernández A. C. Caballero

Varistor

Electroceramics

Abnormal grain growth

10.1016/j.jeurceramsoc.2005.03.175

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Citations (43)

Thick film ZnO based varistors prepared by screen printing

Journal of the European Ceramic Society (2006)

Miguel Angel de la Rubia López M. Peiteado J.F. Fernández A. C. Caballero Janez Holc

Varistor

Screen printing

10.1016/j.jeurceramsoc.2006.02.016

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Citations (21)

Efecto de la relación área-volumen en la sinterización de varistores cerámicos basados en ZnO-Bi2O3,

Boletín de la Sociedad Española de Cerámica y Vidrio (2002)

M.A. de la Rubia M. Peiteado J.F. Fernández A. C. Caballero

Source

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Citations (1)

Mn3−Zn O4 spinel phases in the Zn–Mn–O system

Acta Materialia (2008)

M. Peiteado Sašo Šturm A. C. Caballero Darko Makovec

Tetragonal crystal system

10.1016/j.actamat.2008.04.024

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Citations (28)

Thermal evolution of ZnO-Bi2O3-Sb2O3 system in the region of interest for varistors

Journal of Materials Science (2006)

M. Peiteado M.A. de la Rubia J.F. Fernández A. C. Caballero

Varistor

Electroceramics

10.1007/s10853-006-7168-5

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Citations (31)

Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING

International Journal of Molecular Sciences (2022)

David G. Calatayud Teresa Jardiel Erica Cordero-Oyonarte A. C. Caballero M. Villegas

In recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, tissue auto-fluorescence under external illumination, together with a weak tissue penetration of low wavelength excitation light, largely restricts the application of the technique. Accordingly, new types of fluorescent labels are currently being investigated and, in this search, phosphorescent nanoparticles promise great potential, as they combine the interesting size-dependent properties of nanoscale materials with a long-lasting phosphorescence-type emission that allows optical imaging well after excitation (so avoiding autofluorescence). In this work, core-shell structures consisting of SrAlO:Eu,Dy luminescent cores encapsulated within a biocompatible silica shell were prepared, showing a green persistent phosphorescence with an afterglow time of more than 1000 s. A high-energy ball milling procedure was used to reduce the size of the starting phosphors to a size suitable for cellular uptake, while the silica coating was produced by a reverse micelle methodology that eventually allows the excitation and emission light to pass efficiently through the shell. Confocal fluorescence microscopy using HeLa cancer cells confirmed the potential of the all-ceramic composites produced as feasible labels for in vitro optical imaging.

Autofluorescence

Fluorescence-lifetime imaging microscopy

Persistent luminescence

10.3390/ijms23063410

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Citations (17)

Soft solution processing of ZnO nanoarrays by combining electrophoretic deposition and hydrothermal growth

Materials Chemistry and Physics (2013)

M. Verde M. Peiteado M. Villegas B. Ferrari A. C. Caballero

Electrophoretic Deposition

Deposition

Hydrothermal Synthesis