Gabriela de Morais Gouvêa Lima

Universidade Estadual Paulista (Unesp)

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Cristiane Yumi Koga‐Ito

Universidade Estadual Paulista (Unesp)

Konstantin Georgiev Kostov

Universidade Estadual Paulista (Unesp)

Aline Chiodi Borges

Universidade Estadual Paulista (Unesp)

Thalita Mayumi Castaldelli Nishime

Leibniz Institute for Plasma Science and Technology

Aline Vidal Lacerda Gontijo

Anhanguera-Uniderp University

Ana Lia Anbinder

Universidade Estadual Paulista (Unesp)

Yasmin Rodarte Carvalho

Universidade Estadual Paulista (Unesp)

Renata Mendonça Moraes

Universidade Estadual Paulista (Unesp)

Gabriela de Fátima Santana-Melo

Universidade Estadual Paulista (Unesp)

Mônica Fernandes Gomes

Universidade Estadual Paulista (Unesp)

Cooperative Institutions

Universidade Estadual Paulista (Unesp)

Universidade de São Paulo

Fundação Faculdade de Odontologia

Institute of Science and Technology

Universidade do Vale do Paraíba

Universidade Paulista

Departamento de Ciência e Tecnologia

Prefeitura Municipal de Campos dos Goytacazes

Fundação de Apoio à Universidade Federal de São Paulo

Núcleo de Pesquisas Aplicadas (Brazil)

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Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease

Molecules (2021)

Gabriela de Morais Gouvêa Lima Aline Chiodi Borges Thalita Mayumi Castaldelli Nishime Gabriela de Fátima Santana-Melo Konstantin Georgiev Kostov

Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.

Streptococcus gordonii

10.3390/molecules26185590

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Citations (21)

Low-Grade Central Osteosarcoma in Mandible: Case Report

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology (2014)

Renata Rodrigues Acay Walter Niccoli‐Filho Vivian Narana Ribeiro El Achkar Gabriela de Morais Gouvêa Lima Estela Kaminagakura

Osteoid

Mandible (arthropod mouthpart)

Pleomorphism (cytology)

10.1016/j.oooo.2013.12.103

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Citations (0)

Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study

PLoS ONE (2018)

Aline Chiodi Borges Gabriela de Morais Gouvêa Lima Thalita Mayumi Castaldelli Nishime Aline Vidal Lacerda Gontijo Konstantin Georgiev Kostov

The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity.

Nystatin

Vero cell

Viability assay

10.1371/journal.pone.0199832

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Citations (46)

Ellagic Acid–Cyclodextrin Complexes for the Treatment of Oral Candidiasis

Molecules (2021)

Aline da Graça Sampaio Aline Vidal Lacerda Gontijo Gabriela de Morais Gouvêa Lima Maria Alcionéia Carvalho de Oliveira Laura Soares Souto Lepesqueur

The increase in the prevalence of fungal infections worldwide and the rise in the occurrence of antifungal resistance suggest that new research to discover antifungal molecules is needed. The aim of this study was to evaluate the potential use of ellagic acid–cyclodextrin complexes (EA/HP-β-CD) for the treatment of oral candidiasis. First, the effect of EA/HP-β-CD on C. albicans planktonic cells and biofilms was evaluated. Then, the cytotoxicity of the effective concentration was studied to ensure safety of in vivo testing. Finally, the in vivo effectiveness was determined by using a murine model of induced oral candidiasis. Data was statistically analyzed. The minimal inhibitory concentration of EA/HP-β-CD was 25 µg/mL and a concentration of 10 times MIC (250 µg/mL) showed an inhibitory effect on C. albicans 48 h-biofilms. The complex at concentration 250 µg/mL was classified as slightly cytotoxic. In vivo experiments showed a reduction in fungal epithelial invasion after treatment with EA/HP-β-CD for 24 h and 96 h when compared to the negative control. In conclusion, the results demonstrated that EA/HP-β-CD has antifungal and anti-inflammatory effects, reducing the invasive capacity of C. albicans, which suggests that EA/HP-β-CD may be a promising alternative for the treatment of oral candidiasis.

Ellagic Acid

IC50

10.3390/molecules26020505

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Citations (19)

Cold Atmospheric Pressure Plasma Is Effective against P. gingivalis (HW24D-1) Mature Biofilms and Non-Genotoxic to Oral Cells

Applied Sciences (2022)

Gabriela de Morais Gouvêa Lima Maria Celina Peixoto Lima Aline Chiodi Borges Thalita Mayumi Castaldelli Nishime Cézar Augusto Villela da Silva

The effects of helium cold atmospheric pressure plasma (He-CAPP) jet on Porphyromonas gingivalis (HW24D-1) biofilm, on human gingival fibroblasts (HGF) and human gingival keratinocytes (OBA-9) were assessed. Standardized suspension of P. gingivalis was obtained, and biofilms were grown anaerobically for 48 h. After exposition to He-CAPP, the biofilm viability was evaluated by XTT assay. HGF were grown at 37 °C, in an CO2 chamber in DMEM, while OBA-9 cells were cultured in keratinocyte serum-free medium. After 24 h, plates were exposed to He-CAPP for 1 to 7 min. Plasma was generated using a commercial AC power supply with amplitude modulated signal (voltage amplitude of 20 kVp-p, frequency of 31.0 kHz and duty cycle of 22%). The corresponding discharge power was 0.6W at He flow rate of 1 L/min. DNA damage was accessed by static cytometry. Data were analyzed by GraphPad Prism (p < 0.05). Significant reductions in P. gingivalis viability in relation to non-treated groups were detected (p < 0.0001), directly proportional to exposure time. Treated groups were slightly aneuploid after 5- and 7-min treatment in HGF, and for 3 min in OBA-9 cells, with 1.2 DNA index mean. Helium cold atmospheric pressure plasma jet showed inhibitory effect on P. gingivalis mature biofilm and was not genotoxic for epithelial gingival cells and human oral fibroblasts.

Comet Assay

10.3390/app12147247

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Citations (12)

Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms

Applied Sciences (2021)

Maria Alcionéia Carvalho de Oliveira Gabriela de Morais Gouvêa Lima Thalita Mayumi Castaldelli Nishime Aline Vidal Lacerda Gontijo Beatriz Rossi Canuto de Menezes

The presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for E. faecalis and after 3 min for MRSA. A reduction in P. aeruginosa biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care.

Enterococcus faecalis

MTT assay

Viability assay

Comet Assay

10.3390/app11125441

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Citations (12)

Cold Atmospheric Pressure Plasma Jet Reduces Trichophyton rubrum Adherence and Infection Capacity

Mycopathologia (2019)

Aline Chiodi Borges Thalita Mayumi Castaldelli Nishime Sabrina de Moura Rovetta Gabriela de Morais Gouvêa Lima Konstantin Georgiev Kostov

10.1007/s11046-019-00375-2

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Citations (13)

An Isolated Phlebolith on the Lip: An Unusual Case and Review of the Literature

Case Reports in Pathology (2015)

Gabriela de Morais Gouvêa Lima Renata Mendonça Moraes Ana Sueli Rodrigues Cavalcante Yasmin Rodarte Carvalho Ana Lia Anbinder

Background . Calcified thrombi are a common finding, especially in the pelvic veins. There are generally multiple thrombi, and they are generally associated with vascular malformations. Design . Herein we report a rare case of a single labial phlebolith, not associated with any other vascular lesion. We aim to alert clinicians to the possibility of the occurrence of vascular thrombi in the mouth and to describe the clinical and histological characteristics of such lesions in order to simplify the diagnosis and treatment. Furthermore, we have reviewed the English-language literature published since 1970 reporting oral (including masticatory muscles) phleboliths. Results . Twenty-nine cases of phleboliths have been reported in the literature since 1970. Only three of the reported phleboliths were solitary and not associated with other vascular lesions, as in the case presented here. Conclusion . Although phleboliths not associated with other vascular lesions are not common, clinicians should be aware of the existence of this pathology and include it as differential diagnosis of oral lesions.

10.1155/2015/507840

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Citations (14)

Alternative therapies for denture stomatitis treatment: in vivo experimental model in rats

Brazilian Dental Science (2017)

Guilherme Rodrigues Teodoro Gabriela de Morais Gouvêa Lima Marina Bacelar Silva Patricia Carla Lopes Cybelle Mori Hiraoka

Background: Denture stomatitis (DS) is a multifactorial condition that commonly affects denture users and is mainly caused by Candida albicans. Due to the toxic effects of antifungal therapy, new therapies for DS are claimed. Objective: The aim of the study was to evaluate the efficacy of aqueous extract of Buchenavia tomentosa and sodium bicarbonate against C. albicans in a model of DS in rats. Material and Methods: An acrylic resin device simulating a denture base was fixed covering the palate of forty-eight male rats followed by candidiasis induction. Rats were divided into 4 groups (n=12): Control, sodium bicarbonate, B. tomentosa and nystatin (positive control). Each group was subdivided according to the period of treatment; 24 hours (n=6) and 48 hours (n=6). Animals were sacrificed and had their devices removed for C. albicans counts and SEM analysis. The palate mucosa was removed and processed for histopathologic analysis. Results: After 24 hours of treatment, both B. tomentosa and nystatin groups reduced significantly C. albicans counts when compared to control (nystatin x control, p<0.01; B. tomentosa x control, p=0.03). The results were confirmed by the histologic analysis. Conclusion: Both the aqueous extract of B. tomentosa and sodium bicarbonate was able to significantly decrease C. albicans counts in an experimental model of DS.

Nystatin

Sodium bicarbonate

10.14295/bds.2017.v20i1.1368

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Citations (3)

Evaluation of different periods of estrogen replacement onset in the tibia of ovariectomized rats

Aging Clinical and Experimental Research (2014)

Marianne Spalding Priscila Ferreira Amschlinger Luana Marotta Reis de Vasconcellos Gabriela de Morais Gouvêa Lima Warley David Kerbauy

Estrogen replacement therapy

10.1007/s40520-014-0268-1

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Citations (4)