Chia-Chen Ko

National Taiwan University

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Yu‐Chih Liu

Chang Gung Memorial Hospital

Lu‐Ping Chow

Vision Australia

Fong‐Chi Cheng

University of Pennsylvania

Po‐Tsang Huang

Kaohsiung Armed Forces General Hospital

Chih-Ta Chen

National Taiwan University

Kai-Chao Hsu

National Taiwan University

Kuo‐Long Lou

National Taiwan University

Yun‐Ju Chen

E-Da Hospital

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National Taiwan University

National Taiwan University Hospital

National Cheng Kung University

University of Zagreb

Universidade Federal de São Carlos

Universidade de São Paulo

Institute of Molecular Biology, Academia Sinica

Chang Gung Memorial Hospital

Chang Gung University

Keelung Chang Gung Memorial Hospital

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Chemical Proteomics Identifies Heterogeneous Nuclear Ribonucleoprotein (hnRNP) A1 as the Molecular Target of Quercetin in Its Anti-cancer Effects in PC-3 Cells

Journal of Biological Chemistry (2014)

Chia-Chen Ko Yun‐Ju Chen Chih-Ta Chen Yu‐Chih Liu Fong‐Chi Cheng

Quercetin, a flavonoid abundantly present in plants, is widely used as a phytotherapy in prostatitis and prostate cancer. Although quercetin has been reported to have a number of therapeutic effects, the cellular target(s) responsible for its anti-cancer action has not yet been clearly elucidated. Here, employing affinity chromatography and mass spectrometry, we identified heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) as a direct target of quercetin. A specific interaction between quercetin and hnRNPA1 was validated by immunoblotting and in vitro binding experiments. We found that quercetin bound the C-terminal region of hnRNPA1, impairing the ability of hnRNPA1 to shuttle between the nucleus and cytoplasm and ultimately resulting in its cytoplasmic retention. In addition, hnRNPA1 was recruited to stress granules after treatment of cells with quercetin for up to 48 h, and the levels of cIAP1 (cellular inhibitor of apoptosis), an internal ribosome entry site translation-dependent protein, were reduced by hnRNPA1 regulation. This is the first report that anti-cancer effects of quercetin are mediated, in part, by impairing functions of hnRNPA1, insights that were obtained using a chemical proteomics strategy.

Heterogeneous nuclear ribonucleoprotein

Proteome

10.1074/jbc.m114.553248

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Identification of a novel competitive inhibitor of p38α MAPK by a human PBMC screen

Biochemical and Biophysical Research Communications (2006)

Yu‐Chih Liu Chia-Chen Ko Fong‐Chi Cheng Po‐Tsang Huang Kuo‐Long Lou

IC50

10.1016/j.bbrc.2006.11.069

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Citations (1)