V. M. Stakhanova

Research Field

Studies aimed at the development of a variant of ELISA for the determination of hepatitis B virus surface antigen (HBsAg) showed the assay to be most effective when a modified periodate method of conjugation of highly purified horseradish peroxidase with the IgG-fraction of antiserum to HBsAg was used. With the resulting conjugates, the "sandwich"-test on polystyrene solid phase could detect HBsAg in concentrations up to 5 ng/ml which is several thousand-fold higher in sensitivity than counter immunoelectrophoresis, one order of magnitude more sensitive than the passive hemagglutination test, and comparable with the radioimmunoassay (RIA). The specificity of the method was confirmed by positive results of the neutralization test. By this method, HBsAg in the sera of hepatitis B patients was detected as frequently as by the RIA.

Horseradish peroxidase

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Respiratory viruses of horses, pigs and ducks and their relationship to human influenza virus.

V. M. Stakhanova

Equine influenza

H5N1 genetic structure

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An experimental model of hepatitis A inMacaca mulatta infected with human hepatitis a virus

Bulletin of Experimental Biology and Medicine (1990)

Z V Shevtsova R I Krylova N. V. Doroshenko I. B. Lomovskaya E. G. Belova

Hepatitis virus

10.1007/bf00841423

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[Clinical picture of congenital Cytomegalovirus infection in infants in their first year of life].

PubMed (1992)

Vitaliy Matveev N M Kazinets A A Son'kina V. M. Stakhanova

A study was made of the clinical picture of congenital cytomegaloviral infection in 40 children of the first year of life. The diagnosis was established on the basis of demonstration in all the patients of IgM antibodies to cytomegalovirus (IgM anti-CMV) by EIA with the aid of the commercial test systems. Besides, 81% of the children were found to have cytomegalic-specific cells in urine, 17.6% in saliva, and 72.5% demonstrated IgG anti-CMV (in accordance with EIA). The majority of the patients (70%) were born premature. The most typical clinical signs of congenital cytomegaloviral infection were adynamia, jaundice, liver and CNS injury, prenatal hypotrophy; 45% of the children had developmental abnormalities and stigmas of dysembryogenesis. In 97.5% of the mothers, the pregnancy ran a pathological course. The disease history often pointed to miscarriages (10%) and death of the children due to intrauterine infection in the early times after birth (15%).

Cytomegalovirus

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Use of human tissue culture in in vivo investigations of the aetiology of virus B hepatitis.

Problems of Virology (1974)

V. A. Ananiev V. M. Stakhanova N N Zhilina O. B. Shumkina S. I. Balnokina

Etiology

Hepatitis virus

Liver tissue

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Detection of virus antigens in an infected cell using I131-labeled antibodies

Bulletin of Experimental Biology and Medicine (1964)

V. M. Stakhanova E. M. Zhantieva

10.1007/bf00784419

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Spontaneous and Experimental Hepatitis A in Old World Monkeys

Journal of Medical Primatology (1988)

Z V Shevtsova Б. А. Лапин N. V. Doroshenko R.I. Krilova L.I. Korzaja

Virologic, serologic, biochemical, and morphological data characterizing spontaneous hepatitis A (HA) in cynomolgus macaques (Macaca fascicularis) and green monkeys (Cercopithecus aethiops) are reported. Experimental HA was induced in macaques as a result of infection with human hepatitis A virus (HAV-h). Disease similar to human HA was induced in cynomolgus macaques by HAV isolates from spontaneously sick rhesus (M. mulatta) and green monkeys. This experimental model of HA in macaques can be used for vaccine and anti-viral preparation testing.

10.1111/j.1600-0684.1988.tb00380.x

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Non-Specific Influenza Virus Inhibitors in the Sera of Pyrexial Patients.

Problems of virology (1957)

V. M. Stakhanova

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[Some aspects of the study of human cancer by means of virological methods].

PubMed (1962)

V. M. Zhdanov V. M. Stakhanova