Daniel D. Loeb

Cincinnati Children's Hospital Medical Center

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Clyde A. Hutchison

J. Craig Venter Institute

Marshall H. Edgell

University of North Carolina at Chapel Hill

Lorraine Everitt

University of North Carolina at Chapel Hill

Frank H. Burton

University of Minnesota Medical Center

Matthew Zackoff

Cincinnati Children's Hospital Medical Center

Ronald Swanstrom

Communities In Schools of Orange County

Mary B. Comer

University of Oxford

Maya Dewan

Cincinnati Children's Hospital Medical Center

Richard W. Padgett

Rutgers, The State University of New Jersey

Eric B. Lewellyn

St. Norbert College

Cooperative Institutions

University of North Carolina at Chapel Hill

Cincinnati Children's Hospital Medical Center

University of Wisconsin–Madison

UNC Lineberger Comprehensive Cancer Center

University of Cincinnati

University of Cincinnati Medical Center

University of California, San Francisco

Duke Medical Center

Duke University Hospital

Children's Medical Center

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Experience informed procedural skills training

The Clinical Teacher (2024)

Daniel D. Loeb Andrew J. Lautz Jacob Fleck Matthew Zackoff

Paediatric critical care (PCC) physicians must perform several emergent procedures independently and competently-requiring transition from novice to competent over a 3-year fellowship. However, skill acquisition is not uniform. Individualised training, adapted to the unique experiences and requirements of each trainee, may enhance competency.

Modalities

10.1111/tct.13719

Cite

Citations (1)

Mutations affecting hepadnavirus plus-strand DNA synthesis dissociate primer cleavage from translocation and reveal the origin of linear viral DNA

Journal of Virology (1991)

Silvija I. Staprans Daniel D. Loeb Don Ganem

Hepadnaviruses replicate their circular DNA genomes via reverse transcription of an RNA intermediate. The initial product of reverse transcription, minus-strand DNA, contains two copies of a short direct repeat (DR) sequence, termed DR1 and DR2. Plus-strand DNA synthesis initiates at DR2 on minus-strand DNA, using as a primer a short, DR1-containing oligoribonucleotide derived by cleavage and translocation from the 5' end of pregenomic RNA. To clarify the sequence requirements for plus-strand primer cleavage and translocation, we have constructed mutants of the duck hepatitis B virus bearing base changes in or around the DR1 sequence in the primer. A point mutation at the terminal nucleotide of DR1 has a striking phenotype: normal levels of duplex viral DNA are produced, but nearly all of the DNA is linear rather than circular. Mapping of the 5' end of plus-strand DNA reveals that primer cleavage occurs with normal efficiency and accuracy, but the primer is not translocated to DR2; rather, it is extended in situ to generate duplex linear DNA. Other mutations just 3' to DR1 similarly affect primer translocation, although with differing efficiencies. Linear DNA found in wild-type virus preparations has the same fine structure as the mutant linears described above. These results indicate that (i) plus-strand primer cleavage and translocation are distinct steps that can be dissociated by mutation, (ii) lesions in sequences not included in the primer can severely inhibit primer translocation, and (iii) elongation of such untranslocated primers is responsible for the variable quantities of linear DNA that are found in all hepadnaviral stocks.

Primer (cosmetics)

Primase

Coding strand

10.1128/jvi.65.3.1255-1262.1991

Cite

Citations (163)

15 Reverse Transcription Pathway of the Hepatitis B Viruses

Cold Spring Harbor Monograph Archive (1993)

Daniel D. Loeb Don Ganem

All genetic elements that carry out reverse transcription must solve a common set of problems to effect this complex reaction. These include (1) directing the synthesis of the appropriate RNA template and of the reverse transcriptase itself, (2) assembling these components into a replication complex, (3) initiating and elongating first-strand DNA synthesis on the RNA template, (4) degrading the RNA of the resulting DNA-RNA hybrid to allow the first DNA strand to template the synthesis of the second, and (5) priming and completing second-strand DNA synthesis to generate a duplex DNA product. Until recently, these reactions were understood in detail only for retroviruses (and for retrotransposons like yeast Ty elements whose overall mechanism is closely similar). However, recent studies of reverse transcription in hepatitis B viruses (HBVs) have led to an understanding of this reaction that is nearly as detailed as that of cognate events in retroviral replication. Contrary to expectation, these studies have shown that at nearly every step in the reverse transcription pathway, HBVs have solved the aforementioned common problems in remarkably different ways. In this chapter, we review what is currently known about reverse transcription in HBVs, with particular reference to divergences from the retroviral paradigm. VIRUS STRUCTURE AND REPLICATION: AN OVERVIEW Human HBV is the prototype member of a distinctive family of viruses called hepadnaviruses (for hepa totropic DNA viruses); related viruses exist among several other species, including woodchucks, ground squirrels, and ducks (Marion 1988). All hepadnaviruses replicate preferentially (but not exclusively) in the liver, where they...

Retrotransposon

Transcription

10.1101/087969382.23.329

Cite

Citations (10)

A Side Chain at Position 48 of the Human Immunodeficiency Virus Type-1 Protease Flap Provides an Additional Specificity Determinant

Virology (1995)

Mark D. Moody Steve C. Pettit Wei Shao Lorraine Everitt Daniel D. Loeb

Aspartic acid

Glutamic acid

Cleavage (geology)

Alanine