Johanna Martins-Luna

Peruvian University of Applied Sciences

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Juana del Valle-Mendoza

Peruvian University of Applied Sciences

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Miguel Ángel Aguilar-Luis

Peruvian University of Applied Sciences

Wilmer Silva-Caso

Peruvian University of Applied Sciences

Luís J. del Valle

Universitat Politècnica de Catalunya

Hugo Carrillo‐Ng

Peruvian University of Applied Sciences

Yordi Tarazona-Castro

Peruvian University of Applied Sciences

Ronald Aquino-Ortega

Peruvian University of Applied Sciences

Isaac Peña-Tuesta

Instituto de Investigación Nutricional

Angela Cornejo‐Tapia

Peruvian University of Applied Sciences

Fernando Soto-Febres

Hospital Base Guillermo Almenara Irigoyen

Cooperative Institutions

Peruvian University of Applied Sciences

Hospital Base Guillermo Almenara Irigoyen

Instituto de Investigación Nutricional

Universidad Peruana Cayetano Heredia

Dirección Regional de Salud del Callao

University of Malaya

Konyang University

National University of Cajamarca

Hospital Nacional Cayetano Heredia

Health Protection Research Unit in Emerging and Zoonotic Infections at University of Liverpool

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Dataset SARS-CoV2-cytokines y2021m02 [50]

Figshare (2021)

Juana del Valle-Mendoza Yordi Qesler Tarazona Castro Alfredo Merino‐Luna Hugo Carrillo‐Ng Hugo Carrillo‐Ng

Comparison of cytokines levels among COVID-19 patients living at sea level and high altitude ABSTRACT Objective: The objective of this study was to compare the levels of pro-inflammatory cytokines (IL-6, IL-2, IL-10, INF-α and IFN-γ) in COVID-19 patients and healthy subjects, residing in two cities of Peru at different altitudes. Results: A total of 35 COVID-19 patients and 10 healthy subjects were recruited from each study site. The mean levels of IL-6 (p<0.03) and TNF-α (p<0.01) were significantly different among the study groups. In the case of IL-6, patients from Lima had a mean level of 16.2 pg/ml (healthy) and 48.3 pg/ml (COVID-19), meanwhile, patients from Huaraz had levels of 67.3 pg/ml (healthy) and 97.9 pg/ml (COVID-19). Regarding TNF-α, patients from Lima had a mean level of 25.9 pg/ml (healthy) and 61.6 pg/ml (COVID-19), meanwhile, patients from Huaraz had levels of 89.0 pg/ml (healthy) and 120.6 pg/ml (COVID-19). The levels of IL-2, IL-10 and IFN-γ) were not significantly different in the study groups.

10.6084/m9.figshare.14050610

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Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru

Iranian Journal of Microbiology (2021)

Juana del Valle-Mendoza Cristina del Valle-Varga Ronald Aquino-Ortega Luís J. del Valle Erico Cieza-Mora

Background and Objectives: Pertussis is an infectious disease caused by the Gram-negative bacterium Bordetella pertussis. In Peru, actual public health programs indicate that vaccination against B. pertussis must be mandatory and generalized, besides all detected cases must be reported. The objective of this study was to determine the prevalence of B. pertussis among children under five years of age with a presumptive diagnosis of whopping cough in Cajamarca, a region located in northern Peru. Materials and Methods: The population of this cross-sectional study were children under 5 years old hospitalized as presumptive cases of pertussis during December 2017 to December 2018. The nasopharyngeal samples were analyzed by real-time PCR for the detection of B. pertussis.

Leukocytosis

Whooping cough

Lymphocytosis

Leukopenia

10.18502/ijm.v13i1.5488

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Impact of Adding a Rapid PCR-Based Blood Culture Identification Panel to the Antimicrobial Stewardship Program of Patients with Febrile Neutropenia in a Peruvian Referral Hospital

Antibiotics (2023)

Giancarlo Pérez‐Lazo Juana del Valle-Mendoza Roxana Sandoval‐Ahumada Fernando Soto-Febres Raúl Castillo-Córdova

The addition of Biofire® FilmArray® Blood Culture Identification panel 2 (BCID2) to the antimicrobial stewardship program (ASP) could improve outcomes in bloodstream infections (BSI) of patients with febrile neutropenia (FN). A pre- and post-quasi-experimental single-center study was conducted at a reference hospital in Peru. Three groups were considered: patients with BSI before ASP intervention (control group), patients with BSI after ASP intervention (group 1), and patients with BSI after ASP intervention plus BCID2 PCR Panel implementation (group 2). Overall, 93 patients were identified (32 control, 30 group 1, 31 group 2). The median time to effective therapy was significantly shorter in group 2 compared to group 1 and control group, respectively (3.75 vs. 10 h, p = 0.004; 3.75 vs. 19 h, p < 0.001). No significant differences in terms of relapse of bacteremia, in-hospital mortality (all cause), and 30-day-all-cause hospital readmission between the three study periods were found. The appropriateness of empirical antimicrobial use, adding or change, and the following de-escalation or discontinuation was significant when the two intervention periods were compared with the control group (p < 0.001). In addition to the lack of local studies documenting the microbiological profile of FN episodes, adding syndromic panels-based testing could allow for the consolidation of ASP strategies.

Antimicrobial Stewardship

Bacteremia

Blood Culture

Discontinuation

10.3390/antibiotics12040648

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Unlocking low- and middle-income countries to detect SARS-CoV-2

medRxiv (Cold Spring Harbor Laboratory) (2021)

Roberto Alcántara Katherin Peñaranda Gabriel Mendoza-Rojas Jose A. Nakamoto Johanna Martins-Luna

Summary Low- and middle-income countries (LMICs) are significantly affected by SARS-CoV-2, partially due to their limited capacity for local development of molecular testing and accentuated by the international supply shortage. Here, we describe a molecular toolkit that can be readily produced and deployed in LMICs using minimal laboratory equipment. Our results show that mid-scale production of enzymes and nucleic acids can supply thousand tests per production batch. One-step RT-PCR was optimized for two SARS-CoV-2 loci and coupled to CRISPR/Cas12a detection. The clinical validation indicated a sensitivity near 100% for mid and high viral load samples (Cq ≤ 33). The specificity was around 100% regardless of viral load. The toolkit was used with the mobile laboratory BentoLab, potentially unlocking LMICs to implement detection services in unattended regions. Altogether, we provide detailed methods and performance evidence of molecular tools aiming to aid LMICs to deploy molecular testing for current or future pathogenic outbreaks. One Sentence Summary We describe a molecular toolkit for the detection of SARS-CoV-2 that is compatible with available facilities in low- and middle-income countries (LMICs).

Economic shortage

Molecular diagnostics

2019-20 coronavirus outbreak

10.1101/2021.04.14.21255479

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Leptospira spp. and Rickettsia spp. as pathogens with zoonotic potential causing acute undifferentiated febrile illness in a central-eastern region of Peru

BMC Research Notes (2024)

Wilmer Silva-Caso Miguel Ángel Aguilar-Luis Walter Espinoza-Espíritu Mercedes Vilcapoma-Balbin Luís J. del Valle

Abstract Objetive this study was to determine the relationship between acute febrile illness and bacterial pathogens with zoonotic potential that cause emerging and re-emerging diseases in a central-eastern region of Peru. Results Out of the 279 samples analyzed, 23 (8.2%) tested positive for infection by Rickettsia spp., while a total of 15 (5.4%) tested positive for Leptospira spp. Women had a higher frequency of infection by Rickettsia spp., with 13 cases (53.3%), while men had a higher frequency of infection by Leptospira spp., with 10 cases (66.7%). The most frequently reported general symptom was headache, with 100.0% ( n = 23) of patients with Rickettsia (+) and 86.7% ( n = 13) of patients with Leptospira (+) experiencing it. Arthralgia was the second most frequent symptom, reported by 95.6% ( n = 22) and 60% ( n = 9) of patients with Rickettsia (+) and Leptospira (+), respectively. Myalgia was reported by 91.3% ( n = 21) and 66.7% ( n = 10) of patients with Rickettsia (+) and Leptospira (+), respectively. Retroocular pain, low back pain, and skin rash were also present, but less frequently. Among the positives, no manifestation of bleeding was recorded, although only one positive case for Leptospira spp. presented a decrease in the number of platelets.

myalgia

10.1186/s13104-024-06837-1

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Detection of SARS-CoV-2 IgM Antibodies in Febrile Patients From an Endemic Region of Dengue and Chikungunya

International Journal of Infectious Diseases (2022)

Yordi Tarazona-Castro L. Troyes Johanna Martins-Luna F. Cabellos-Altamirano Miguel Ángel Aguilar-Luis

Immunoglobulin M

10.1016/j.ijid.2021.12.128

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Identfication of viral and bacterial etiologic agents of the pertussis-like syndrome in children under 5 years old hospitalized

BMC Infectious Diseases (2019)

Stephanie Saiki-Macedo Jorge Valverde-Ezeta Angela Cornejo‐Tapia María E. Castillo Verónica Petrozzi-Helasvuo

Acute respiratory infections (ARIs) represent an important cause of morbidity and mortality in children, remaining a major public health concern, especially affecting children under 5 years old from low-income countries. Unfortunately, information regarding their epidemiology is still limited in Peru.A secondary data analysis was performed from a previous cross-sectional study conducted in children with a probable diagnosis of Pertussis from January 2010 to July 2012. All samples were analyzed via Polymerase Chain Reaction (PCR) for the following etiologies: Influenza-A, Influenza-B, RSV-A, RSV-B, Adenovirus, Parainfluenza 1 virus, Parainfluenza 2 virus, Parainfluenza 3 virus, Mycoplasma pneumoniae and Chlamydia pneumoniae.A total of 288 patients were included. The most common pathogen isolated was Adenovirus (49%), followed by Bordetella pertussis (41%) from our previous investigation, the most prevelant microorganisms were Mycoplasma pneumonia (26%) and Influenza-B (19.8%). Coinfections were reported in 58% of samples and the most common association was found between B. pertussis and Adenovirus (12.2%).There was a high prevalence of Adenovirus, Mycoplasma pneumoniae and other etiologies in patients with a probable diagnosis of pertussis. Despite the presence of persistent cough lasting at least two weeks and other clinical characteristics highly suspicious of pertussis, secondary etiologies should be considered in children under 5 years-old in order to give a proper treatment.

Etiology

Medical microbiology

Whooping cough

10.1186/s12879-019-3671-6

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Citations (21)

Oropouche virus infection in patients with acute febrile syndrome: Is a predictive model based solely on signs and symptoms useful?

PLoS ONE (2022)

Hilda V. Durango-Chavez Carlos J. Toro‐Huamanchumo Wilmer Silva-Caso Johanna Martins-Luna Miguel Ángel Aguilar-Luis

Oropouche fever is an infectious disease caused by the Oropouche virus (OROV). The diagnosis and prediction of the clinical picture continue to be a great challenge for clinicians who manage patients with acute febrile syndrome. Several symptoms have been associated with OROV virus infection in patients with febrile syndrome; however, to date, there is no clinical prediction rule, which is a fundamental tool to help the approach of this infectious disease.To assess the performance of a prediction model based solely on signs and symptoms to diagnose Oropouche virus infection in patients with acute febrile syndrome.Validation study, which included 923 patients with acute febrile syndrome registered in the Epidemiological Surveillance database of three arbovirus endemic areas in Peru.A total of 97 patients (19%) were positive for OROV infection in the development group and 23.6% in the validation group. The area under the curve was 0.65 and the sensitivity, specificity, PPV, NPV, LR + and LR- were 78.2%, 35.1%, 27.6%, 83.6%, 1.20 and 0.62, respectively.The development of a clinical prediction model for the diagnosis of Oropouche based solely on signs and symptoms does not work well. This may be due to the fact that the symptoms are nonspecific and related to other arbovirus infections, which confuse and make it difficult to predict the diagnosis, especially in endemic areas of co-infection of these diseases. For this reason, epidemiological surveillance of OROV in various settings using laboratory tests such as PCR is important.

10.1371/journal.pone.0270294

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Citations (16)

Leptospirosis in febrile patients with suspected diagnosis of dengue fever

BMC Research Notes (2021)

Juana del Valle-Mendoza Carlos Palomares-Reyes Hugo Carrillo‐Ng Yordi Tarazona-Castro Hugo Carrillo‐Ng

Abstract Objective This study was carried out to determine the prevalence of leptospirosis among febrile patients with a suspicious clinical diagnosis of dengue fever in northern Peru. Results A total of 276 serum samples from patients with acute febrile illness (AFI) and suspected diagnosis for dengue virus (DENV) were analyzed. We identified an etiological agent in 121 (47.5%) patients, DENV was detected in 30.4% of the cases, leptospirosis in 11.2% and co-infection by both pathogens was observed in 5.9% of the patients. In this study the most common clinical symptoms reported by the patients were: headache 89.1%, myalgias 86.9% and arthralgias 82.9%. No differences in symptomatology was observed among the different study groups.

Etiology

10.1186/s13104-021-05627-3

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Citations (13)

Variations in cervico-vaginal microbiota among HPV-positive and HPV-negative asymptomatic women in Peru

BMC Research Notes (2021)

Hugo Carrillo‐Ng Lorena Becerra-Goicochea Yordi Tarazona-Castro Luis Pinillos-Vilca Luís J. del Valle

Abstract Objective To characterize the cervicovaginal microbiota of HPV-positive and HPV-negative asymptomatic Peruvian women, by identifying the presence of 13 representative bacteria genus. Results A total of 100 HPV-positive and 100 HPV-negative women were matched by age for comparison of microbiota. The following bacteria were more frequently identified in HPV-positive patients compared to HPV-negative: Eubacterium (68 vs 32%), Actinobacteria (46 vs 33%), Fusobacterium (11 vs 6%) and Bacteroides (20 vs 13%). A comparison between high-risk and low-risk genotypes was performed and differences were found in the detection of Actinobacteria (50 vs 33.33%), Bifidobacterium (50 vs 20.83%) and Enterococcus (50 vs 29.17%).

Eubacterium

10.1186/s13104-020-05422-6

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Citations (9)