Persistent odontogenic infections caused by resistant bacterial species, such as , have consistently been associated with deep-seated infections. This in vitro study aimed to evaluate the antibacterial and antivirulence effects of kale () on . was freshly incubated in tryptic soy broth media. Three experiments per concentration of kale were conducted under aseptic conditions (i.e., disc diffusion, broth microdilution, and reverse-transcription polymerase chain reaction) to evaluate the antibacterial and antivirulence effects. The samples were then treated with 1000, 500, 250, 125, 65, 30, 15, 7, and 3 mg/mL kale; ampicillin (positive control); and tryptic soy broth (negative control). After 24-h incubation, the inhibition zone, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and relative gene expression of the virulence factor (intermedilysin []) were measured. All assays were conducted in triplicate. The findings were reported and analyzed as means ± standard deviations. The agar disc diffusion and relative gene expression were statistically analyzed using one-way analysis of variance and Tukey’s test, with the significance level set at P < 0.05. Kale showed antibacterial effects on by significantly inhibiting bacterial growth and reducing expression only at a concentration of 1000 mg/mL; it yielded an inhibition zone of 11.12 ± 1.59 mm, which was smaller than that with ampicillin. The MIC and MBC ranged from 15 to 65 mg/mL and from 500 mg/mL, respectively. Conversely, the highest concentration of kale yielded significantly less inhibition than did ampicillin. The antibacterial effects of kale may be dose-dependent. Kale can inhibit bacterial growth and suppress expression under in vitro conditions of , which is mainly involved in deep-seated odontogenic infections.
Objective: To examine the anti-gastric ulcer activity of Payawanorn (Psuderanthemum palatiferum) water extract (PPE) in rats.Material and Methods: Evaluation of anti-gastric ulcer activity of PPE was performed using three induced gastric ulcers models: (1) ethanol/hydrochloric acid (EtOH/HCl), (2) restraint water immersion stress and (3) indomethacin. Ulcer indices were determined by microscopic examination (10X). Pylorus ligation and gastric-wall mucus determination were used to investigate the mechanism of anti-gastric ulcer activity of PPE.Results: Oral administration of PPE (150, 300 and 600 mg/kg) significantly inhibited gastric ulcer formation induced by EtOH/HCl, by water immersion restraint stress, and by indomethacin in rats (p-value<0.05). PPE at 600 mg/kg showed a high percent inhibition of gastric ulcer formation in all models. In the pylorus-ligated model, pretreatment with PPE had no effect on pH, acidity output or the gastric acid secretion rate, demonstrating that PPE lacks an anti-secretory effect. Gastric wall mucus was markedly preserved by pretreatment PPE at 600 mg/kg (18.91±2.57 μg alcian blue/g wet stomach) after ethanol-induced ulcer when compared to that the control group (12.38±1.02 μg alcian blue/ g wet stomach).Conclusion: PPE possesses an anti-gastric ulcer effect related to the preservation of gastric mucus, supporting the traditional use of P. palatiferum to treat gastric ulcers.
Antioxidant activities of 10 essential oils and 10 absolutes extracted from Thai aromatic plants were evaluated and compared to thyme oil, trolox, quercetin, and kaempferol by two independent assays: the 2, 2-diphenyl-1-1-picrylhydrazyl (DPPH*) radical scavenging assay and the thiobarbituric acid reactive species (TBARS) assay for lipid peroxidation. We found that four essential oils including ginger oil (Zingiber officinale Roscoe), Wan-sao-long leaf oil (Amomum uliginosum Koen), lemongrass oil (Cymbopogon citratus), holy basil oil (Ocimum sanctum L.), and the absolute of dwarf ylang-ylang [Cananga odorata Hook. f. & Thomson var. fruticosa (Craib) J. Sinclair] exhibited high antioxidant activity in both DPPH and TBARS assays and possessed satisfactory fragrance properties. These were then combined into an essential oil blend (EOB) and retested for antioxidant activity. The EOB also exhibited high antioxidant activity in the above assays. It was then incorporated into a stable cream base as EOB body cream. The EOB body cream was found to be best able under storage in stress conditions and presented significantly higher antioxidant activity than its' cream base both before and after stability testing. The effect of EOB body cream on skin surface topography was evaluated in 29 healthy volunteers using the Skin Visiometer (SV 600 FW, CK Electronic GmbH, Germany). Three parameters, Ra, Rz (roughness), and surface, were analyzed. After 4 weeks of application, the EOB body cream showed significant reductions in surface and Rz compared with before treatment (p < 0.05, paired t-test), and with untreated and placebo treatment (p < 0.05, Duncan test). These results indicate that the essential oils and absolutes from Thai plants may serve as potential sources of natural antioxidants for spa and cosmetic products designed to prevent or treat signs of skin aging.
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In this study, the essential oil of Anethum graveolens L. seed (AEo) was studied for their chemical composition, antioxidant potential, antimicrobial and anticancer activities. AEo was obtained from hydro-distillation and their composition was analyzed by GC-MS. The major compositions of AEo are D -carvone, carvone, dill apiol and limonene. AEo was investigated for antioxidant activity by DPPH, ABTS, FRAP assay and measured total phenolic content by Folin-Ciocalteu colorimetric method. Agar-well diffusion assay was used to study the anti-microbial activity and also agar-dilution and broth-micro dilution techniques were employed for minimum inhibitory concentration (MIC) determination. In addition, the cytotoxicity and anticancer activity were evaluated on Green fluorescent protein (GFP) and Resazurin micro plate assay (REMA). The results showed that AEo exhibited high total phenolic content (GAE= 4.5746 mg/mL) and antioxidant activities on DPPH (TEAC= 52.5391 mg/mL), ABTS (TEAC= 1.5936 mg/mL) and FRAP assay (TEAC= 0.5469 mg/mL) and also showed potent activity against Staphylococcus aureus and Escherichia coli at the MIC= 5.99 mg/mL. Furthermore, AEo presented non-cytoxicity in normal cell whereas it exhibited greatly anti-cancer activity on KB-Oral cavity and MCF7-Breast cancer cells. Keywords: Anethum graveolens L., antioxidant activity, antimicrobial activity, anticancer activity, chemical compositions
Tongkat ali (Eurycoma longifolia Jack) (ELJ) is a plant in the Simaroubaceae family. Its roots are used in traditional Thai medicine to treat inflammation, pain, and fever; however, the antiulcer abilities of its ethanolic extract have not been studied. This study examined the anti-inflammatory, antinociceptive, antipyretic, and gastroprotective effects of ethanolic ELJ extract in animal models and found that ELJ effectively reduced EPP-induced ear edema in a dose-dependent manner and that a high dose of ELJ inhibited carrageenan-induced hind paw edema formation. In cotton-pellet-induced granuloma formation, a high dose of ELJ suppressed the increases in wet granuloma weight but not dry or transudative weight. In the formalin-induced nociception study, ELJ had a significant dose-dependent inhibitory impact. Additionally, the study found that yeast-induced hyperthermia could be significantly reduced by antipyretic action at the highest dose of ELJ. In all the gastric ulcer models induced by chemical substances or physical activity, ELJ extracts at 150, 300, and 600 mg/kg also effectively prevented gastric ulcer formation. In the pyloric ligation model, however, the effects of ELJ extract on gastric volume, gastric pH, and total acidity were statistically insignificant. These findings support the current widespread use of Eurycoma longifolia Jack in traditional medicine, suggest the plant’s medicinal potential for development of phytomedicines with anti-inflammatory, antinociceptive, and antipyretic properties, and support its use in the treatment of gastric ulcers due to its gastroprotective properties.
External parasites, particularly ticks and fleas, are among the most common problems affecting dogs. Chemical medicines are commonly used to prevent and eliminate such external parasites, but their improper use can cause adverse reactions, and the toxins they contain may remain in the environment.
The present study was designed to evaluate the antibacterial and antioxidant activities of hexane, ethylacetate and ethanol extracts of Leea rubra (Leeaceae) roots and stems, which has been used as a Lanna Traditional Medicines for Mahoog. Each extract was tested for antibacterial activity by agar diffusion method and microbroth dilution method and antioxidant activity by 2,2´-azino-bis(3-ethyl- benzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The ethylacetate extract of L. rubra root showed the highest antibacterial activity against gram-positive (IZD=15.5.0±0.5 to 17.5±0.5 mm, MIC=0.098-1.562 mg/ml). While the ethanolic extract of root showed the strongest antioxidant activity in ABTS, DPPH and FRAP method (TEAC=0.888±0.001, 0.849±0.020 and 0.733±0.037, respectively). The data obtained from this study confirms the traditional use of L. rubra for treatment Mahoog. Keywords: Leea rubra , antioxidant activity, antibacterial activity
Cissus quadrangularis Linn. (CQ) is a medicinal plant with good evidence for the treatment of hemorrhoids, listed in the Thai National List of Herbal Products in the oral dosage form. Acmella paniculata (Wall ex. DC.) R. K. Jansen. (AP) is a medicinal plant with a local anesthetic effect.To investigate the potential of rectal suppositories containing CQ and AP extracts to alleviate symptoms of hemorrhoids compared with the commercialized rectal suppository containing hydrocortisone and cinchocaine.Hemorrhoid outpatients (n = 105) with different severity grades (I, II, or III) from eight hospitals in northern Thailand were included in this study. Hemorrhoid severity was graded by proctoscopy associated with either anal pain or bleeding related to hemorrhoids or both. The patients were randomly allocated to two groups: CQ-AP group (n = 52) or the commercialized rectal suppository group (n = 53). One suppository was rectally administered twice daily in the morning and at bedtime for seven days. Evaluations were performed by physicians on days 1, 4, and 8 of the study. The primary endpoints were bleeding and prolapse size, while the secondary endpoint was anal pain.Baseline demographics, lifestyle, constipation, number of prolapses, grade of hemorrhoid severity, and duration of experiencing hemorrhoids were comparable in both groups of patients. The effects of CQ-AP and the commercialized rectal suppository on bleeding, prolapse size, and anal pain were comparable. The patients in both groups were satisfied with both products at comparable levels and stated a preference for further use in the case of hemorrhoids recurrence. In terms of safety, the patients in the commercialized rectal suppository group experienced a higher incidence of adverse events, including anal pain and bleeding.Rectal suppositories containing a combined extract of CQ and AP show potential in alleviating hemorrhoidal symptoms with a good safety profile.
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