Manjula Bhanoori

Osmania University

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S. Shivaji

L V Prasad Eye Institute

Suresh Govatati

University of Tennessee Health Science Center

Mamata Deenadayal

Osmania University

Swapna Siddamalla

Osmania University

Praveen Guruvaiah

University of Alabama at Birmingham

Sravanthi Malempati

Krishna University

Varadacharyulu Nallanchakravarthula

Sri Krishnadevaraya University

Gadiparthi N. Rao

University of Tennessee Health Science Center

Tumu Venkat Reddy

Osmania University

Perugu Shyam

National Institute of Technology Warangal

Cooperative Institutions

Osmania University

Centre for Cellular and Molecular Biology

University of Glasgow

Association of Research Libraries

University of Hong Kong

Florida Institute of Technology

University of Tennessee Health Science Center

National Institute of Nutrition

University of Hyderabad

Indian Council of Medical Research

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Sepia pharaonis Ink Mitigates Dehydroepiandrosterone-Induced Insulin Resistance in Mouse Model of Polycystic Ovarian Syndrome

Pathophysiology (2024)

Prathyusha Yamarthi Rama Satyasri Kotipalli Samatasai Patnaik Kv Veena M Kathirvel

The present study aims to evaluate the effect of Sepia pharaonis ink on insulin resistance in PCOS-induced mice. Treatment with sepia ink in dehydroepiandrosterone (DHEA)-induced PCOS mice at various doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight mitigated the insulin resistance in the study groups with decreased concentration of testosterone and increased concentrations of estrogen and progesterone compared to the PCOS group tested by ELISA. The histopathological analysis and restoration of glucose analysis showed a significant reduction in treatment groups. Reduced expression of insulin resistance genes like androgen receptor (AR), insulin receptor substrate 1 (IRS-1), and insulin-like growth factor1 (IGF-1) by qRT-PCR indicate a positive impact of sepia ink in alleviating the symptoms associated with PCOS. Taken together, the results of this study indicate sepia ink as a promising therapeutic intervention and a possible drug target for insulin resistance in diabetes and gynecological disorders like PCOS.

Sepia

10.3390/pathophysiology31030031

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Genetic Variants of VDR and PGC-1α Are Not Associated with the Risk of Endometriosis in Indian Women

DNA and Cell Biology (2022)

Himabindu Beeram Swapna Siddamalla Venkat Reddy Tumu Veena KV Akanksha Vidala

An aberrant immunologic mechanism and mitochondrial biogenesis have been suggested to be involved in the pathogenesis of endometriosis. Genetic alterations in the vitamin D receptor (VDR) gene and peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) may lead to important defects in gene activation, which principally affect immune function and normal mitochondrial function. Therefore, we hypothesized a possible role of VDR and PGC-1α genes in the pathogenesis of endometriosis and analyzed the association of genetic variants ApaI A/C (rs7975232) and TaqI T/C (rs731236) of VDR and rs8192678 (G/A), rs13131226 (T/C), and rs2970856 (T/C) of PGC-1α gene. This study included a total of 425 reproductive-age women (cases = 200 and controls = 225). Detection of VDR and PGC-1α gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism and sequencing analysis. The chi-square test was used to compare allele and genotype frequencies between groups, and a p-value of <0.05 was considered statistically significant. The genotype and allele distribution of both the gene polymorphisms did not show statistically significant association with endometriosis. Our result indicated ApaI A and TaqI T of VDR and GTT of PGC-1α gene as the most common haplotype in Indian women. The data suggest that VDR and PGC-1α gene polymorphisms did not play an important role in the pathogenesis of endometriosis in Indian women studied.

TaqI

Pathogenesis

10.1089/dna.2022.0350

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Citations (3)

The Interleukin-6 –174G/C Promoter Polymorphism Is Not Associated With Endometriosis in South Indian Women

Journal of the Society for Gynecologic Investigation (2005)

Manjula Bhanoori Kusuma Arvind Babu Mamata Deenadayal Stephen Kennedy S. Shivaji

Genotype frequency

10.1016/j.jsgi.2005.03.005

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Citations (38)

Association of genetic variations in phosphatase and tensin homolog (PTEN) gene with polycystic ovary syndrome in South Indian women: a case control study

Archives of Gynecology and Obstetrics (2020)

Swapna Siddamalla Suresh Govatati Veena Kunjumol Venu Nagendram Erram Mamata Deenadayal

Tensin

Hyperandrogenism

Linkage Disequilibrium

10.1007/s00404-020-05658-4

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Citations (4)

Association of E-cadherin single nucleotide polymorphisms with the increased risk of endometriosis in Indian women

Molecular Human Reproduction (2011)

Suresh Govatati Naveen Kumar Tangudu Mamata Deenadayal Baidyanath Chakravarty S. Shivaji

The objective of the present study was to investigate the association between gene E-cadherin single nucleotide polymorphisms (SNPs) and risk of developing endometriosis in Indian women and to evaluate the role of E-cadherin expression in the pathophysiology of endometriosis. A genetic association study was conducted in 715 endometriosis cases and 500 controls of Indian origin. We genotyped -160 C/A, +54 C/T and -347 G/GA SNPs of gene E-cadherin by PCR-sequencing and PCR-restriction fragment length polymorphism techniques. Haplotype frequencies for multiple loci and the standardized disequilibrium coefficient (D') for pair-wise linkage disequilibrium (LD) were assessed by Haploview Software. In addition, to better understand genetic contributions to the pathophysiology of endometriosis, the expression pattern of E-cadherin in the endometrium of women with and without endometriosis was analyzed by western blot and immunohistochemical analysis. The frequencies of -347GA/GA (P = 0.026) and -160A/A (P = 0.0019) genotypes and -347G/-160A/+54C (P = 0.007) and -347GA/-160A/+54C (P < 0.0001) haplotypes were significantly different between patients and controls. Strong LD was observed between -347G/GA and -160C/A loci (D' = 0.64) when compared with -347G/GA and +54C/T (D' = 0.585) or -160C/A and +54C/T (D' = 0.05) loci in cases. Furthermore, increased membranous E-cadherin expression was observed in cases than in controls. The expression seems to be genotype dependent. In conclusion, the E-cadherin -347GA/GA and -160A/A genotypes and -347GA/-160A/+54C and -347G/-160A/+54C haplotypes may jointly modify the risk of endometriosis in Indian women. In addition, the differential expression of E-cadherin may play an important role in pathogenesis of endometriosis.

Linkage Disequilibrium

Genetic Association

10.1093/molehr/gar079

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Citations (39)

Mitochondrial displacement loop alterations are associated with endometriosis

Fertility and Sterility (2013)

Suresh Govatati Mamata Deenadayal S. Shivaji Manjula Bhanoori

10.1016/j.fertnstert.2013.02.021

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Citations (34)

The vascular endothelial growth factor (VEGF) +405G>C 5′-untranslated region polymorphism and increased risk of endometriosis in South Indian women: a case control study

Human Reproduction (2005)

Manjula Bhanoori K Arvind Babu Pavankumar N.G. Reddy Katragadda Lakshmi Narasimha Rao Krina T. Zondervan

BACKGROUND: Vascular endothelial growth factor (VEGF), a major mediator of angiogenesis and vascular permeability, is known to play a key role in the pathophysiology of endometriosis. METHODS AND RESULTS: The single nucleotide polymorphisms, −460C>T and +405G>C, in the 5′-untranslated region of the VEGF gene were tested for association in a case–control study of 215 affected women and 210 women with no evidence of disease. All the women were of South Indian origin and ascertained from the same infertility clinic. The genotype and allele frequencies of the −460C>T polymorphism did not differ significantly between cases and controls. In contrast, the genotype (P=0.002) and allele (P=0.001) frequencies of the +405G>C polymorphism showed a significant difference between cases and controls. The +405 GG genotype was found more often in patients with an endometrioma >3 cm compared to controls. The frequency of the −460T/+405C haplotype (P=0.016) was significantly lower in affected women compared to controls. CONCLUSIONS: The −460T/+405C haplotype in the VEGF gene, which is associated with lower promoter activity, was significantly less common in women with endometriosis than in controls. These data suggest that the +405G allele may influence the likelihood of a woman developing the disease.

Pathophysiology

10.1093/humrep/deh852

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Citations (86)

Association of eNOS and STAT6 Gene Polymorphisms with the Susceptibility of Polycystic Ovary Syndrome in South Indian Women

Journal of Biomedical Research & Environmental Sciences (2022)

Manjula Bhanoori Veena KV Swapna Siddamalla Praveen Guruvaiah Tumu Venkat Reddy

Purpose: Polycystic Ovary Syndrome (PCOS) is a complex multifactorial endocrine metabolic disorder of reproductive-aged women characterized by hyperandrogenism, hirsutism, anovulation, hyperinsulinemia and polycystic ovaries. The main aim of this study was to investigate the association of eNOS and STAT6 gene Single Nucleotide Polymorphisms (SNPs) with the susceptibility of PCOS in South Indian Women. Research question: What is the association status of eNOS and STAT6 SNPs with PCOS? Methods: The present genetic association study involves clinically confirmed PCOS patients (n = 105) and non-PCOS controls (n = 110) of the Dravidian linguistic group. Genotyping of +894G/T (Glu298Asp) SNP of eNOS and 2964G/A SNP of STAT6 was performed by Polymerase Chain Reaction (PCR) and sequencing analysis. Results: There were statistically significant differences in the genotype and allele frequencies of eNOS+894G/T (p = 0.1110) and STAT62964G/A (0.0019) between the cases and controls, according to codominant, dominant and recessive genotype models. Conclusion: In conclusion, the eNOS and STAT6 gene polymorphisms may constitute an inheritable risk factor for PCOS in South Indian women.

SNP

Hyperandrogenism

10.37871/jbres1393

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Citations (0)

Characterization of Ctr family genes and the elucidation of their role in the life cycle of Neurospora crassa

Fungal Genetics and Biology (2009)

Premsagar Korripally Anand Tiwari Haritha Adhikarla Kiranmayi Patnala Manjula Bhanoori

Conidiation

Crassa

10.1016/j.fgb.2009.12.006

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