Henri Bastos

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

J Testart

Institut National de Santé Publique

Isabelle Allemand

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Bruno Lassalle

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Lydia Riou

CEA Paris-Saclay - Etablissement de Fontenay-aux-roses

Pierre Fouchet

CEA Paris-Saclay - Etablissement de Fontenay-aux-roses

François D. Boussin

Université Paris Cité

Mathieu Coureuil

Université Paris Cité

J. P. Louis

Université de Montpellier

Alexandra Chicheportiche

CEA Paris-Saclay - Etablissement de Fontenay-aux-roses

B. Dutrillaux

Centre National de la Recherche Scientifique

Cooperative Institutions

CEA Paris-Saclay - Etablissement de Fontenay-aux-roses

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Inserm

Université Paris Cité

Université Paris-Saclay

CEA Paris-Saclay

Institut de Radiobiologie Cellulaire et Moléculaire

Stabilité Génétique, Cellules Souches et Radiations

Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes

Centre National de la Recherche Scientifique

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis

Cytometry Part A (2005)

Henri Bastos Bruno Lassalle Alexandra Chicheportiche Lydia Riou J Testart

Abstract Background Spermatogenesis in adult is a complex stepwise process leading to terminally differentiated spermatozoa. The cellular heterogeneity of testis renders complex the studies on molecular aspects of this differentiation process. Analysis of the regulation of adult spermatogenesis would undoubtedly benefit from the development of techniques to characterize each germinal differentiation step. Methods Hoechst 33342 staining of mouse testicular cells allows characterization of an enriched population in germinal stem cell and spermatogonia, called side population. In this study, we examined the definition of the various germinal populations stained by Hoechst 33342, notably meiotic and postmeiotic cells. Results Preleptotene spermatocytes, spermatocyte I, spermatocyte II, and round and elongated spermatids were discriminated by Hoechst 33342 staining. In addition, we associated differentiation of spermatocyte I through leptotene to diplotene with changes in Hoechst 33342 red fluorescence pattern. Conclusions Hoechst 33342 staining of viable germinal cells constitutes a valuable tool to study normal and impaired mouse adult spermatogenesis or to isolate viable cells from various differentiation stages for studies of molecular mechanisms regulating spermatogenesis. © 2005 Wiley‐Liss, Inc.

Spermatocyte

10.1002/cyto.a.20129

Cite

Citations (191)

The Telomerase Activity of Adult Mouse Testis Resides in the Spermatogonial α6-Integrin-Positive Side Population Enriched in Germinal Stem Cells

Endocrinology (2005)

Lydia Riou Henri Bastos Bruno Lassalle Mathieu Coureuil J Testart

Abstract Testis is one of the organs with the most telomerase activity in the adult. This activity protects chromosomes from telomere attrition and ensures the transmission of full-length chromosomes to progeny. Little is known about telomerase activity during adult germ cell differentiation, however. We demonstrate here that the telomerase activity of adult mouse testis resides in the α6-integrin-positive Side Population containing spermatogonia and enriched in spermatogonial stem cells. The telomerase activity of these cells fell upon entry into meiosis and during the subsequent spermiogenesis. In addition, the telomerase activity of cells in various stages of differentiation was unaffected by aging and, notably, remained high in the α6-integrin-positive Side Population.

10.1210/en.2005-0502

Cite

Citations (33)

`Side Population' cells in adult mouse testis expressBcrp1gene and are enriched in spermatogonia and germinal stem cells

Development (2003)

Bruno Lassalle Henri Bastos J. P. Louis Lydia Riou J Testart

Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular cells also display a 'Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor Ko143 show that germinal 'Side Population' phenotype is dependent on BCRP1 activity. Analysis of two well-defined models of altered spermatogenesis (W/Wv mutants and cryptorchid male mice) and RNA expression studies of differentiation markers demonstrate that germinal 'Side Population' contains spermatogonial cells. In addition, alpha 6-integrin and Stra8 germinal stem cell markers, are expressed in the 'Side Population'. In vivo repopulation assay clearly establishes that testis 'Side Population' in adult mice is highly enriched in male germ stem cells.

Side population

10.1242/dev.00918

Cite

Citations (146)