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Haywood Dail Laughinghouse

University of Florida

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David E. Berthold

University of Fort Lauderdale

Forrest W. Lefler

University of Fort Lauderdale

Annick Wilmotte

University of Liège

Solange Bósio Tedesco

Universidade Federal de Santa Maria

Igor Stelmach Pessi

University of Helsinki

Andrielle Wouters Kuhn

Universidade Federal de Santa Maria

Maximiliano Barbosa

University of Fort Lauderdale

Aline Augusti Boligon

Universidade Federal de Santa Maria

Yannick Lara

University of Liège

Viviane Dal-Souto Frescura

Universidade Federal de Santa Maria

Cooperative Institutions

University of Florida

Universidade Federal de Santa Maria

Universidade Federal do Rio Grande do Sul

University of Liège

Universidade de São Paulo

University of Fort Lauderdale

Instituto de Botânica

United States Geological Survey

The Ohio State University

U.S. Army Engineer Research and Development Center

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Spatiotemporal diversity and community structure of cyanobacteria and associated bacteria in the large shallow subtropical Lake Okeechobee (Florida, United States)

Frontiers in Microbiology (2023)

Forrest W. Lefler Maximiliano Barbosa Paul V. Zimba Ashley R. Smyth David E. Berthold

Lake Okeechobee is a large eutrophic, shallow, subtropical lake in south Florida, United States. Due to decades of nutrient loading and phosphorus rich sediments, the lake is eutrophic and frequently experiences cyanobacterial harmful algal blooms (cyanoHABs). In the past, surveys of the phytoplankton community structure in the lake have been conducted by morphological studies, whereas molecular based studies have been seldom employed. With increased frequency of cyanoHABs in Lake Okeechobee (e.g., 2016 and 2018 Microcystis-dominated blooms), it is imperative to determine the diversity of cyanobacterial taxa that exist within the lake and the limnological parameters that drive bloom-forming genera. A spatiotemporal study of the lake was conducted over the course of 1 year to characterize the (cyano)bacterial community structure, using 16S rRNA metabarcoding, with coincident collection of limnological parameters (e.g., nutrients, water temperature, major ions), and cyanotoxins. The objectives of this study were to elucidate spatiotemporal trends of community structure, identify drivers of community structure, and examine cyanobacteria-bacterial relationships within the lake. Results indicated that cyanobacterial communities within the lake were significantly different between the wet and dry season, but not between periods of nitrogen limitation and co-nutrient limitation. Throughout the year, the lake was primarily dominated by the picocyanobacterium Cyanobium. The bloom-forming genera Cuspidothrix, Dolichospermum, Microcystis, and Raphidiopsis were highly abundant throughout the lake and had disparate nutrient requirements and niches within the lake. Anatoxin-a, microcystins, and nodularins were detected throughout the lake across both seasons. There were no correlated (cyano)bacteria shared between the common bloom-forming cyanobacteria Dolichospermum, Microcystis, and Raphidiopsis. This study is the first of its kind to use molecular based methods to assess the cyanobacterial community structure within the lake. These data greatly improve our understanding of the cyanobacterial community structure within the lake and the physiochemical parameters which may drive the bloom-forming taxa within Lake Okeechobee.

Microcystis

Bloom

10.3389/fmicb.2023.1219261

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La ecología de las algas carofíceas (Charales)

Maximiliano Barbosa David E. Berthold Forrest W. Lefler Haywood Dail Laughinghouse

Este documento proporciona una descripción general de la ecología de las carofíceas, los requisitos de hábitat y el estado de las carofíceas en el estado de Florida. También se presentan referencias para la identificación de especies del género Chara. Written by Maximiliano Barbosa, David E. Berthold, Forrest W. Lefler, and H. Dail Laughinghouse IV, and published by the UF/IFAS Agronomy Department, May 2021.

10.32473/edis-ag452-2021

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Use of 454 pyrosequencing protocol for the assessment of cyanobacterial diversity

Igor Stelmach Pessi Pedro De Carvalho Haywood Dail Laughinghouse Annick Wilmotte

Pyrosequencing

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Assessment of bioinformatic pipelines for the analysis of 454 pyrosequencing data using artificial cyanobacterial communities

Igor Stelmach Pessi Pedro De Carvalho Maalouf Haywood Dail Laughinghouse Denis Baurain Annick Wilmotte

Pyrosequencing

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A next-generation approach to assess the cyanobacterial diversity and biogeography in the High Arctic (Svalbard)

Haywood Dail Laughinghouse Igor Stelmach Pessi David Velázquez Annick Wilmotte

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Variability of the chromosomal number and meiotic behavior in populations ofBidens pilosaL. (Asteraceae) from southern Brazil

Caryologia (2008)

Fachinetto Juliana Maria Haywood Dail Laughinghouse Antonio Carlos Ferreira da Silva Solange Bósio Tedesco

Abstract Bidens pilosa is a medicinal species from the Asteraceae family, with a wide geographical distribution, found all over Brazil. There are no reports on chromosomal studies from the Brazilian South. Mitotic chromosomes, meiotic behavior, and pollen viability were studied in 6 populations of B. pilosa from Rio Grande do Sul State, Brazil, using conventional staining with acetic orcein 2%. The populations include cytotypes with a variable number of chromosomes: 2n=36, 2n=48 and 2n=54, indicating polyploidy and aneuploidy. The meiotic process was considered regular, even though irregular chromosomal pairing appeared, which is normal in cytotypes from a polyploid complex. The irregularities observed were mainly on univalent, trivalent, and tetravalent pairing associations. However, the pollen viability estimative was high.

Orcein

Polyploid

Bidens pilosa

10.1080/00087114.2008.10589625

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Atmospheric Progression of Microcystin-LR from Cyanobacterial Aerosols

Environmental Science & Technology Letters (2020)

Myoseon Jang David E. Berthold Zechen Yu Cecilia Silva-Sánchez Haywood Dail Laughinghouse

The potential for exposure to aerosolized algal toxins during harmful algal blooms has not been well studied. A fundamental question about the longevity of algal toxins in the aerosol phase remains. In this study, the influence of environmental factors such as sunlight and atmospheric oxidants (e.g., ozone and OH radicals) on the lifetime of microcystin-LR (MC-LR), which is known to be the most toxic of the MCs produced by cyanobacteria, was investigated. A cyanobacterial culture aliquot (Microcystis aeruginosa and Dolichospermum sp.) spiked with MC-LR was nebulized into a large outdoor photochemical chamber and atmospherically aged in the presence and absence of sunlight or ozone. The concentrations of MC-LR in aerosol were measured using liquid chromatography/tandem mass spectrometry and the enzyme-linked immunosorbent assay (ELISA) for atmospheric aging of aerosols. With 61.5 nm3 m–3 gaseous ozone, the second-order rate constant of ELISA-based MC-LR in M. aeruginosa aerosol was approximately (3.91 ± 0.17) × 105 M–1 s–1 (54 min lifetime). Because of the involvement of OH radicals, the daytime degradation of MC-LR was significantly faster than that at night with the same amount of ozone in the chamber air. We conclude that under natural sunlight, MC-LR in cyanobacterial aerosols quickly decays through heterogeneous chemistry with atmospheric oxidants but can still impact neighboring communities.

Microcystis aeruginosa

10.1021/acs.estlett.0c00464

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The BCCM/ULC collection to conserve the biodiversity and explore the applied potential of Polar cyanobacteria

Pierre Becker P Szternfeld Maja Andjelković R Anthonissen Luc Verschaeve

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The ‘cyanobiome’ of Svalbard, High Arctic

Igor Stelmach Pessi Haywood Dail Laughinghouse David Velázquez Yannick Lara Annick Wilmotte

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Identifying invasive species threats, pathways, and impacts to improve biosecurity

Ecosphere (2023)

Deah Lieurance Susan Canavan Donald C. Behringer Amy E. Kendig Carey R. Minteer

Abstract Managing invasive species with prevention and early‐detection strategies can avert severe ecological and economic impacts. Horizon scanning, an evidence‐based process combining risk screening and consensus building to identify threats, has become a valuable tool for prioritizing invasive species management and prevention. We assembled a working group of experts from academic, government, and nonprofit agencies and organizations, and conducted a multi‐taxa horizon scan for Florida, USA, the first of its kind in North America. Our primary objectives were to identify high‐risk species and their introduction pathways, to detail the magnitude and mechanism of potential impacts, and, more broadly, to demonstrate the utility of horizon scanning. As a means to facilitate future horizon scans, we document the process used to generate the list of taxa for screening. We evaluated 460 taxa for their potential to arrive, establish, and cause negative ecological and socioeconomic impacts, and identified 40 potential invaders, including alewife, zebra mussel, crab‐eating macaque, and red swamp crayfish. Vertebrates and aquatic invertebrates posed the greatest invasion threat, over half of the high‐risk taxa were omnivores, and there was high confidence in the scoring of high‐risk taxa. Common arrival pathways were ballast water, biofouling of vessels, and escape from the pet/aquarium/horticulture trade. Competition, predation, and damage to agriculture/forestry/aquaculture were common impact mechanisms. We recommend full risk analysis for the high‐risk taxa; increased surveillance at Florida's ports, state borders, and high‐risk pathways; and periodic review and revision of the list. Few horizon scans detail the comprehensive methodology (including list‐building), certainty estimates for all scoring categories and the final score, detailed pathways, and the magnitude and mechanism of impact. Providing this information can further inform prevention efforts and can be efficiently replicated in other regions. Moreover, harmonizing methodology can facilitate data sharing and enhance interpretation of results for stakeholders and the general public.

Biosecurity

10.1002/ecs2.4711

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