M. G. S. Vaz

Universidade Federal do Rio de Janeiro

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Carlos Nozawa

Universidade Federal do Ceará

Maria Angélica Arpon Marandino Guimarães

Universidade Federal do Rio de Janeiro

M. G. Von Hubinger

Universidade Federal do Rio de Janeiro

Elisabeth Lampe

Fundação Oswaldo Cruz

Cooperative Institutions

Universidade Federal do Rio de Janeiro

Fundação Oswaldo Cruz

Hospital Universitário Clementino Fraga Filho

Universidade Federal Fluminense

University of Rio Grande and Rio Grande Community College

Instituto Português de Oncologia Francisco Gentil

Czech Academy of Sciences, Institute of Microbiology

Centro de Estudos em Psicologia da Saúde

Universidade Federal do Ceará

Universidade Estadual de Londrina

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Immunofluorescent antibody to rotavirus and antigen excretion in infantile acute diarrhoea

Revista do Instituto de Medicina Tropical de São Paulo (1988)

Carlos Nozawa Elisabeth Lampe M. G. S. Vaz M. G. Von Hubinger

A serological study was carried out to evaluate the causal relationship of acute diarrhoea and rotavirus in children under five years of age. The rotaviral infection was demonstrated in paired sera by determining the antibody titers by immunofluorescence test (IF). Bovine rotavirus-infected MA-104 cell culture was used as substratum for IF. Out of 80 paired sera it was shown that 23 (28.75%) presented seroconversion, 19 (23.75%) samples showed a twofold increase in their titers and 38 (47.5%) had no increase in rotavirus antibody. This result is discussed on the light of previously obtained results on viral antigen detection by counterimmunoelectrosmophoresis (CIEOP).

Seroconversion

Immunofluorescence

10.1590/s0036-46651988000500002

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Detection of Astrovirus-like in diarrhoeic stool and its coexistence with Rotavirus

Revista do Instituto de Medicina Tropical de São Paulo (1985)

Carlos Nozawa M. G. S. Vaz Maria Angélica Arpon Marandino Guimarães

A clinical case is described in this paper in that a 5-month old baby girl severely malnourished and dehydrated presented a prolonged acute diarrhoea. No enteropathogenic bacteria or parasites were demonstrated. Virological study by electron microscopy (EM) showed that the patient shed both astrovirus-like and rotavirus in the watery stool as long as 12 days after the onset. Immune electron microscopy (IEM) performed with the patient serum revealed clumps of both viruses. It is suggest that this may be a case of mixed infection due to astrovirus-like and rotavirus.

Astrovirus

10.1590/s0036-46651985000500002

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Citations (9)