Elena Lonati

University of Milano-Bicocca

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Alessandra Bulbarelli

University of Milano-Bicocca

Paola Palestini

University of Milano-Bicocca

Laura Botto

University of Milano-Bicocca

Emanuela Cazzaniga

University of Milano-Bicocca

Chiara Milani

University of Florence

Francesca Farina

Istituti di Ricovero e Cura a Carattere Scientifico

Massimo Masserini

University of Milano-Bicocca

Giulio Sancini

University of Milano-Bicocca

Luca Massimino

IRCCS Ospedale San Raffaele

Anna Maria Brambilla

ASST Fatebenefratelli Sacco

Cooperative Institutions

University of Milano-Bicocca

University of Milan

Istituti di Ricovero e Cura a Carattere Scientifico

University of Bologna

Universitat Autònoma de Barcelona

University College London

Harvard University

Universitat de Barcelona

University of Palermo

Amsterdam Neuroscience

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Pin1 Contribution to Alzheimer’s Disease: Transcriptional and Epigenetic Mechanisms in Patients with Late-Onset Alzheimer’s Disease

Neurodegenerative Diseases (2012)

Beatrice Arosio Alessandra Bulbarelli Sussy Bastías‐Candia Elena Lonati L. Mastronardi

Background: Neurofibrillary tangles and senile plaques are hallmarks of Alzheimer’s disease (AD) although the molecular basis of their coexistence remains elusive. The peptidyl-prolyl cis/trans isomerase Pin1 acts on both tau and amyloid precursor protein to regulate their functions by influencing tau phosphorylation and amyloid precursor protein processing. Objective: In order to identify potential biomarkers for AD in easily accessible cells and to gain insight into the relationship between the brain and peripheral compartments in AD pathology, we investigated Pin1 expression and activity in the peripheral blood mononuclear cells of subjects with late-onset AD (LOAD) and age-matched controls (CT). Methods: Gene and protein expression, promoter methylation, Ser16 phosphorylation and activity of Pin1 were evaluated in 32 samples from subjects with LOAD and in 28 samples from CT. Results: In LOAD subjects, there was a statistically significant reduction in Ser16 phosphorylation (–30%; p = 0.041) and promoter methylation (–8%; p = 0.001), whereas Pin1 expression was significantly increased (+74%; p = 0.018). Conclusion: The modifications of Pin1 found in LOAD subjects support its involvement in the pathogenesis of the disease with an important role being played by epigenetic mechanisms.

Pathogenesis

Amyloid (mycology)

10.1159/000333799

Cite

Citations (33)

Prion protein structure is affected by pH‐dependent interaction with membranes: A study in a model system

FEBS Letters (2007)

Francesca Re Silvia Sesana Alberto Barbiroli Francesco Bonomi Emanuela Cazzaniga

Interaction of full length recombinant hamster prion protein with liposomes mimicking lipid rafts or non‐raft membrane regions was studied by circular dichroism, chemical cross‐linking and sucrose gradient ultracentrifugation. At pH 7.0, the protein bound palmitoyloleoylphosphatidylcholine/cholesterol/sphingomyelin/monosialoganglioside GM1 (GM1) ganglioside liposomes but not palmitoyloleoylphosphatidylcholine alone (bound/free = 0.33 and 0.01, respectively), maintaining the native α‐helical structure and monomeric form. At pH 5.0, though still binding to quaternary mixtures, in particular GM1, the protein bound also to palmitoyloleoylphosphatidylcholine (bound/free 0.35) becoming unfolded and oligomeric. The pH‐dependent interaction with raft or non‐raft membranes might have implication in vivo, by stabilizing or destabilizing the protein.

Raft

Lipid raft

Ganglioside

10.1016/j.febslet.2007.12.003

Cite

Citations (27)

Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor

Proceedings of the National Academy of Sciences (2010)

Yulia Popova Thayumanavan Palvannan Elena Lonati Margarida Agrochão Johan M. Thevelein

A novel concept in eukaryotic signal transduction is the use of nutrient transporters and closely related proteins as nutrient sensors. The action mechanism of these "transceptors" is unclear. The Pho84 phosphate transceptor in yeast transports phosphate and mediates rapid phosphate activation of the protein kinase A (PKA) pathway during growth induction. We have now identified several phosphate-containing compounds that act as nontransported signaling agonists of Pho84. This indicates that signaling does not require complete transport of the substrate. For the nontransported agonist glycerol-3-phosphate (Gly3P), we show that it is transported by two other carriers, Git1 and Pho91, without triggering signaling. Gly3P is a competitive inhibitor of transport through Pho84, indicating direct interaction with its phosphate-binding site. We also identified phosphonoacetic acid as a competitive inhibitor of transport without agonist function for signaling. This indicates that binding of a compound into the phosphate-binding site of Pho84 is not enough to trigger signaling. Apparently, signaling requires a specific conformational change that may be part of, but does not require, the complete transport cycle. Using Substituted Cysteine Accessibility Method (SCAM) we identified Phe(160) in TMD IV and Val(392) in TMD VIII as residues exposed with their side chain into the phosphate-binding site of Pho84. Inhibition of both transport and signaling by covalent modification of Pho84(F160C) or Pho84(V392C) showed that the same binding site is used for transport of phosphate and for signaling with both phosphate and Gly3P. Our results provide to the best of our knowledge the first insight into the molecular mechanism of a phosphate transceptor.

10.1073/pnas.0906546107

Cite

Citations (147)

Lifestyle Profiles and Their Sociodemographic Correlate in an Academic Community Sample

International Journal of Environmental Research and Public Health (2022)

Roberta Adorni Elena Lonati Francesco Zanatta Michael Belingheri Massimiliano Rossetti

Promoting healthy behaviors throughout life is an essential prevention tool. Prior research showed that unhealthy behaviors tend to co-occur and interplay. However, which behaviors co-occur most frequently and which sociodemographic variables are associated with specific clusters of unhealthy behavior are still being determined. This study aimed to identify different lifestyle profiles and analyze their associations with sociodemographic factors in an Italian academic community to plan targeted initiatives to promote healthy lifestyles. A sample of 8715 adults from an Italian university (mean age = 26 years; range = 18–76; 30% male) participated in an online survey in 2019. Four health-related behaviors were evaluated: diet, physical activity, smoking, and alcohol consumption. Lifestyle profiles were identified through cluster analysis. Then, a multinomial logistic regression was performed to explore the association among lifestyle profiles, sociodemographic variables (age, gender, and academic role), and body mass index (BMI). Results showed that older age was associated with the probability of belonging to the profile characterized by smoke addiction and regular alcohol consumption but also with the healthiest diet. The younger the age, the greater the probability of belonging to the most physically active profile. Men were more likely than women to belong to the lifestyle profile with the most regular alcohol consumption and the highest physical activity. Lower BMI was associated with the most physically active profile. This study shed light on factors associated with different co-occurring health-related behaviors that should be considered in planning effective communication strategies and preventive health interventions within the academic community.

Screen Time

10.3390/ijerph20010231

Cite

Citations (2)

Enhanced folate binding of cultured fibroblasts from Alzheimer's disease patients

Neuroscience Letters (2008)

Emanuela Cazzaniga Alessandra Bulbarelli Elena Lonati Francesca Re Gloria Galimberti

Folate receptor

10.1016/j.neulet.2008.03.046

Cite

Citations (11)

P3–343: Effect of abeta (25–35) peptide on rat cultured hippocampal neurons: A neuroprotective response?

Alzheimer s & Dementia (2006)

Alessandra Bulbarelli Emanuela Cazzaniga Elena Lonati Massimo Masserini

Alzheimer disease (AD) is characterized by the accumulation of brain extracellular plaques composed of Abeta peptide and by hyperphosphorylation of Tau protein, leading to intracellular neurofibrillary tangles. Abeta, and its toxic 25–35 fragment, plays a central role in neuronal death, inducing apoptosis. During brain development, neurotrophins (NTs), in particular nerve growth factor (NGF), brain–derived neurotrophic factor, neurotrophin–3 (NT–3) and neurotrophin–4/5 (NT–4/5), antagonize cell death by promoting neuronal survival acting on a set of high affinity tyrosine kinase receptors (i.e. TrkA, TrkB, and TrkC), that, when activated, are the docking sites for different kinases such as phosphatidylinositol 3–kinase (PI–3K). For these reasons neurotrophins may represent a potential candidate for therapeutic treatment of neurobiological disorders. In the present study we are investigating the effect of Abeta peptide (fragment 25–35) on protein expression and phosphorylation, The investigation has been carried out using cultured rat primary hippocampal neurons. After incubation for different times (3 min to 48 hours) with 25μM Abeta, cells were subjected to RT–PCR and EF/WB experiments. RT–PCR results indicate that treatments with Abeta induce an increased expression of TrkA ≫ NGF > p75, and of the Bcl2 family–Bcl–XL genes. Interestingly, an unidentified 50 KDa protein is present after Abeta treatments. Assessment of protein phosphorylation reveals, 1–3 hours after Abeta treatment, an increase of protein phosphorylation, in particular within the 75–200 and 15–25 KDa range. Moreover, a 2–fold increase of anti–apoptotic phospho–Akt and of its substrate, phospho–GSK–3β, was observed. Since it is known that TrkA/NGF–PI3K/Akt cell survival pathway affects GSK–3 beta phosphorylation, reducing tau hyperphosphorylation, it could be hypothesized that at least in a first stage, hippocampal cells exposed to Abeta try to activate survival mechanisms through the modification of protein expression/phosphorylation.

Hyperphosphorylation

10.1016/j.jalz.2006.05.1613

Cite

Citations (0)

Study of the antioxidant effects of coffee metabolites in C6 glioma cells exposed to diesel exhaust particles

Paola Palestini Alessandra Bulbarelli Elena Lonati Emanuela Cazzaniga T Carozzini

Source

Cite

Citations (0)

Neutral lipid reshaping in Blood Brain Barrier is induced in modelled ischemia-reperfusion.

Elena Lonati C. De Paola Zoi Stefania M Gigliola Tatiana Carrozzini

Source

Cite

Citations (0)

Abeta Peptide Toxicity is Reduced After Treatments Decreasing Phosphatidylethanolamine Content in Differentiated Neuroblastoma Cells

Neurochemical Research (2011)

Emanuela Cazzaniga Alessandra Bulbarelli Elena Lonati Antonina Orlando Francesca Re

Phosphatidylethanolamine

Choline

Phosphocholine

Immunostaining

Neurotoxicity

Amyloid (mycology)

10.1007/s11064-011-0415-4

Cite

Citations (22)

International Journal of Environmental Research and Public Health (2024)

Elena Lonati Emanuela Cazzaniga Roberta Adorni Francesco Zanatta Michael Belingheri

The transition to higher education at University is a critical moment for young adults to acquire unhealthy habits regarding physical activity (PA) and adherence to a healthy diet. Negative behaviors might be maintained in the years to come with a major risk of suffering from a Non-Communicable Disease. This study aims to determine the relationship between diet and PA in the student community of University of Milano-Bicocca. Students between 18 and 30 years old completed an online survey (6949 students). Two analyses of covariance (ANCOVA), chi-square tests of independence, and a binomial logistic regression were performed to examine the relationship between adequacy of food consumption and PA, in association also with sociodemographic characteristics. Data show a strong correlation between behaviors analyzed, with a proportional positive association between PA and healthy diet. Nevertheless, a third of the sample students incur in incorrect habits for both diet and PA. Further, students performing intensive PA have the healthiest food consumption in general but the worst red meat and pork intake. Accordingly, men practice more PA but have a less adequate diet, exactly contrary to women. In conclusion, policies promoting consciousness of well-being would transform Universities into healthy hubs for virtuous habits.

Consumption

10.3390/ijerph21050626

Cite

Citations (5)