Jiayu Wan

Chinese Academy of Agricultural Sciences

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Shengjun Bu

Changchun University of Science and Technology

Wensen Liu

Academy of Military Medical Sciences

Zhuo Hao

Chinese Academy of Agricultural Sciences

Zhongyi Li

Commonwealth Scientific and Industrial Research Organisation

Chuanjing Ju

First Automotive Works (China)

Kuiyu Wang

Sun Yat-sen University

Na Xu

Jilin Medical University

Hongguo Wei

Changchun University of Science and Technology

Hongyu Zhou

Renji Hospital

Chengyou Ma

Jilin University

Cooperative Institutions

Academy of Military Medical Sciences

Jilin University

Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Jilin Agricultural University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

Peking University

Zhejiang University

University of Chinese Academy of Sciences

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A pregnancy test strip for detection of pathogenic bacteria by using concanavalin A-human chorionic gonadotropin-Cu3(PO4)2 hybrid nanoflowers, magnetic separation, and smartphone readout

Microchimica Acta (2018)

Shengjun Bu Kuiyu Wang Chuanjing Ju Ye Han Zhongyi Li

Human chorionic gonadotropin

Pregnancy test

10.1007/s00604-018-2968-2

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Citations (100)

A Colorimetric Immunosensor Based on Hemin@MI Nanozyme Composites, with Peroxidase-like Activity for Point-of-care Testing of Pathogenic E. coli O157:H7

Analytical Sciences (2020)

Yan Leng Shengjun Bu Zhong‐Yi Li Zhuo Hao Chengyou Ma

Recently, nanozymes have become a topic of particular interest due to their high activity level, stability and biocompatibility. In this study, a visual, sensitive and selective point-of-care immunosensor was established to test the pathogen Escherichia coli O157:H7 (E. coli O157:H7). Hemin and magainin I (MI) hybrid nanocomposites (Hemin@MI) with peroxidase-mimicking activities were synthesized via a "one-pot" method, involving the simple mixing of an antimicrobial peptide (MI) against E. coli O157:H7 and hemin in a copper sulfate sodium phosphate saline buffer. Hemin@MI nanocomposites integrating target recognition and signal amplification were developed as signal probes for the point-of-care colorimetric detection of pathogenic E. coli O157:H7. Hemin@MI nanocomposites exhibit excellent peroxidase activity for the chromogenic reaction of ABTS, which allows for the visual point-of-care testing of E. coli O157:H7 in the range of 102 to 108 CFU/mL, with a limit of detection of 85 CFU/mL. These data suggest this immunosensor provides accessible and portable assessments of pathogenic E. coli O157:H7 in real samples.

Hemin

ABTS

10.2116/analsci.20p081

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Citations (4)

Click Chemistry Actuated Exponential Amplification Reaction Assisted CRISPR–Cas12a for the Electrochemical Detection of MicroRNAs

ACS Omega (2022)

Hongguo Wei Shengjun Bu Ze Wang Hongyu Zhou Xue Li

MicroRNAs (miRNAs) play a very important role in biological processes and are used as biomarkers for the detection of a variety of diseases, including neurodegenerative diseases, chronic cardiovascular diseases, and cancers. A sensitive point-of-care (POC) method is crucial for detecting miRNAs. Herein, CRISPR-Cas12a combined with the click chemistry actuated exponential amplification reaction was introduced into an electrochemical biosensor for detecting miRNA-21. The target miRNA-21 initiated the click chemistry-exponential amplification reaction in the electrochemical biosensor to produce numerous nucleic acid fragments, which could stimulate the trans-cleavage ability of CRISPR-Cas12a to cleave hairpin DNA electrochemical reporters immobilized on the electrode surface. Under optimal conditions, the minimum detection limit for this electrochemical biosensor was as low as 1 fM. Thus, the proposed electrochemical biosensor allows sensitive and efficient miRNA detection and could be a potential analysis tool for POC test and field molecular diagnostics.

10.1021/acsomega.2c01930

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Citations (8)

Distribution and drug resistance of bacteria in swine house environment.

Zhongguo shouyi xuebao (2010)

Nan Li Wensen Liu Jing Xu Linna Liu Jiping Li

A total of 296 strains were isolated from air and feces in 4 swine houses in Jilin province.Identification results showed there were 76.7% gram positive bacteria and 23.3% gram negative bacteria using MALDI-TOF mass spectrometry.The isolates were detected for antimicrobial resistance patterns by Vitek-32 System.There was a high resistance rate against penicillins,tetracyclines,erythromycin,et al.in coagulase negative staphylococci.94% Escherichia coli were multiple drug resistant strains.29.4% of them were extended spectrum β-lactamase productions and susceptible to cephamycins,carbapenems,compound praeparatum only.Over 35% resistance was observed to ampicillin and cotrimoxazole among non-fermentation bacteria.The study demonstrated that pathogenic bacteria and drug resistant bacteria could be a threat in some swine houses of Jilin.It will be very significant to enhance surveillance for rational using antibiotics and preventing dissemination of drug resistant bacteria.

Pathogenic bacteria

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Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie

Prion (2014)

Sha Li Chuanjing Ju Chao Han Zhongyi Li Wensen Liu

Previous studies have demonstrated that Shadoo (Sho), a GPI-linked glycoprotein encoded by the Sprn gene with a membrane localization similar to PrP(C), is reduced in the brains of rodents with terminal prion disease. To determine the functional significance of Sho in prion disease pathogenesis, Sho-deficient mice were generated by gene targeting. Sho knockout and control wild-type (WT) mice were infected with themouse-adapted scrapie strains 22L or RML. No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice. In this model of prion disease, Sho removal had no effect on disease pathogenesis.

Knockout mouse

10.4161/19336896.2014.971574

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Citations (1)

Catabolite control protein A has an important role in the metabolic regulation of Streptococcus suis type 2 according to iTRAQ-based quantitative proteomic analysis

Molecular Medicine Reports (2015)

Xulong Lang Wan Zhong-hai Ying Pan Bu ZhaoYang Xiuran Wang

The catabolite control protein A (ccpA) regulates the carbon metabolism in Streptococcus suis type 2 and has pleiotropic regulatory functions in bacterial virulence and transcription. The present study systematically investigated ccpA activity in Streptococcus suis type 2 using isobaric tag for relative and absolute quantification (iTRAQ) liquid chromatography‑tandem mass spectrometry‑based proteomics. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses demonstrated that ccpA is an important protein for the regulation of metabolism, virulence and immune pathways in Streptococcus suis type 2. The present study therefore expanded the current understanding of the effects of ccpA on virulence, metabolic regulation and transcription in Streptococcus suis type 2 and other important pathogens.

CCPA

Streptococcus suis

KEGG

Isobaric labeling

Streptococcus Pyogenes

10.3892/mmr.2015.4211

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Citations (3)

Visual detection of nucleic acids based on lateral flow biosensor and hybridization chain reaction amplification

Talanta (2016)

Na Ying Chuanjing Ju Zhongyi Li Wensen Liu Jiayu Wan

Streptavidin