Francesco Orsini

Istituto Ortopedico Gaetano Pini

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A. Lascialfari

University of Pavia

Paolo Arosio

University of Milan

G. Poletti

University of Milan

Claudio Sangregorio

University of Florence

Claudia Innocenti

Institute for the Chemistry of OrganoMetallic Compounds

M. Corti

University of Pavia

Manuel Mariani

Istituto Nazionale di Fisica Nucleare, Sezione di Milano

Cristina Lenardi

University of Milan

C̃

C. ̃Riccardi

University of Milano-Bicocca

M. Santacroce

University of Milan

Cooperative Institutions

University of Milan

144

National Interuniversity Consortium of Materials Science and Technology

University of Pavia

University of Florence

Centre National de la Recherche Scientifique

University of Milano-Bicocca

Mylan (Switzerland)

Istituto Nazionale di Fisica Nucleare, Sezione di Milano

Istituti di Ricovero e Cura a Carattere Scientifico

Istituto Nazionale di Fisica Nucleare, Sezione di Pavia

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Tumor Necrosis Factor-α Inhibition Before and After Rituximab Treatment in Juvenile Idiopathic Arthritis: What Shall We Expect? A Pilot Study

The Journal of Rheumatology (2022)

Achille Marino Francesco Orsini Francesca Pregnolato Rolando Cimaz

Rituximab (RTX), a chimeric monoclonal antibody against B cell antigen CD20, has been used in patients with recalcitrant juvenile idiopathic arthritis ( JIA) with some efficacy. 1,2 The resetting of peripheral B cells promoted by RTX represents quite an interesting option in autoimmune diseases such as JIA, not only for the immediate effect but also for the possible use of other therapies that might be necessary to achieve disease control.

10.3899/jrheum.211039

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Citations (1)

Observing Xenopus laevis oocyte plasma membrane by atomic force microscopy

Methods (2009)

Francesco Orsini M. Santacroce Paolo Arosio Vellea Franca Sacchi

10.1016/j.ymeth.2009.12.002

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Citations (8)

How Xylenol Orange and Ferrous Ammonium Sulphate Influence the Dosimetric Properties of PVA–GTA Fricke Gel Dosimeters: A Spectrophotometric Study

Gels (2022)

Martina Scotti Paolo Arosio Elisa Brambilla Salvatore Gallo Cristina Lenardi

The development of Fricke gel (FG) dosimeters based on poly(vinyl alcohol) (PVA) as the gelling agent and glutaraldehyde (GTA) as the cross-linker has enabled significant improvements in the dose response and the stability over time of spatial radiation dose distributions. However, a standard procedure for preparing FG in terms of reagent concentrations is still missing in the literature. This study aims to investigate, by means of spectrophotometric analyses, how the sensitivity to the radiation dose and the range of linearity of the dose-response curve of PVA-GTA-FG dosimeters loaded with xylenol orange sodium salt (XO) are influenced by ferrous ammonium sulphate (FAS) and XO concentrations. Moreover, the effect of different concentrations of such compounds on self-oxidation phenomena in the dosimeters was evaluated. PVA-GTA-FG dosimeters were prepared using XO concentrations in the range 0.04-0.80 mM and FAS in the range 0.05-5.00 mM. The optical absorbance properties and the dose response of FG were investigated in the interval 0.0-42.0 Gy. The results demonstrate that the amount of FAS and XO determines both the sensitivity to the absorbed dose and the interval of linearity of the dose-response curve. The study suggests that the best performances of FG dosimeters for spectrophotometric analyses can be obtained using 1.00-0.40 mM and 0.200-0.166 mM concentrations of FAS and XO, respectively.

Xylenol orange

Absorbance

Glutaraldehyde

10.3390/gels8040204

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Citations (21)

NMR Study of Spin Dynamics in V7Zn and V7Ni Molecular Rings

Applied Magnetic Resonance (2020)

Fatemeh Adelnia Paolo Arosio Manuel Mariani Francesco Orsini Alice Radaelli

Abstract We present a 1 H NMR investigation of spin dynamics in two finite integer spin molecular nanomagnetic rings, namely V 7 Zn and V 7 Ni. This study could be put in correlation with the problem of Haldane gap in infinite integer spin chains. While V 7 Zn is an approximation of a homometallic broken chain due to the presence of s = 0 Zn 2+ ion uncoupled from nearest neighbor V 2+ s = 1 ions, the V 7 Ni compound constitutes an example of a closed periodical s = 1 heterometallic chain. From preliminary susceptibility measurements on single crystals and data analysis, the exchange coupling constant J / k B results in the order of few kelvin. At room temperature, the frequency behavior of the 1 H NMR spin–lattice relaxation rate 1/ T 1 allowed to conclude that the spin–spin correlation function is similar to the one observed in semi-integer spin molecules, but with a smaller cutoff frequency. Thus, the high-T data can be interpreted in terms of, e.g., a Heisenberg model including spin diffusion. On the other hand, the behavior of 1/ T 1 vs temperature at different constant fields reveals a clear peak at temperature of the order of J / k B , qualitatively in agreement with the well-known Bloembergen–Purcell–Pound model and with previous results on semi-integer molecular spin systems. Consequently, one can suggest that for a small number N of interacting s = 1 ions ( N = 8), the Haldane conjecture does not play a key role on spin dynamics, and the investigated rings still keep the quantum nature imposed mainly by the low number of magnetic centers, with no clear topological effect due to integer spins.

Coupling constant

10.1007/s00723-020-01281-3

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Citations (1)

Magnetism and spin dynamics of novel encapsulated iron oxide superparamagnetic nanoparticles

Dalton Transactions (2013)

Paolo Arosio Giovanni Baldi Emo Chiellini M. Corti Alberto Dessy

Encapsulated Fe3O4 nanoparticles of average diameters d = 12 nm are obtained by coprecipitation, in the presence of 2-methoxyethanol hemiester of poly(maleic anhydride-alt-butyl vinyl ether) 5% grafted with poly(ethylene glycol) (VP-MAG nanoparticles). A complete characterization of nude and encapsulated nanoparticles through structural techniques (namely XRD, TEM, SEM), Raman spectroscopy and magnetic measurements has been performed. These nanoparticles compared with commercial compounds (ENDOREM®) present superparamagnetic behavior and nuclear relaxivities that make them promising as magnetic resonance imaging (MRI) contrast agents (CAs). We found that our nanostructures exhibit r2 relaxivity higher than those of commercial CAs over the whole frequency range. The MRI efficiency of our samples was related to their microstructural and magnetic properties.

Superparamagnetism

Coprecipitation

Magnetism

10.1039/c3dt32805h

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Citations (5)

Soft X-ray contact microscopy of nematode Caenorhabditis elegans

The European Physical Journal D (2004)

G. Poletti Francesco Orsini D. Batani A. Bernardinello T. Desai

Cuticle (hair)

10.1140/epjd/e2004-00084-4

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Citations (8)

Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study

Journal of Clinical Microbiology (2001)

C. Kirschner K. Maquelin P. Pina N. A. Ngo Thi Lin‐P'ing Choo‐Smith

ABSTRACT Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one of the leading causes of nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] and Raman) could provide potential alternatives to conventional typing methods, because they are fast, easy to perform, and economical. We present a comparative study using phenotypic, genotypic, and vibrational spectroscopic techniques for typing a collection of 18 Enterococcus strains comprising six different species. Classification of the bacteria by Fourier transform (FT)-IR spectroscopy in combination with hierarchical cluster analysis revealed discrepancies for certain strains when compared with results obtained from automated phenotypic systems, such as API and MicroScan. Further diagnostic evaluation using genotypic methods—i.e., PCR of the species-specific ligase and glycopeptide resistance genes, which is limited to the identification of only four Enterococcus species and 16S RNA sequencing, the “gold standard” for identification of enterococci—confirmed the results obtained by the FT-IR classification. These results were later reproduced by three different laboratories, using confocal Raman microspectroscopy, FT-IR attenuated total reflectance spectroscopy, and FT-IR microspectroscopy, demonstrating the discriminative capacity and the reproducibility of the technique. It is concluded that vibrational spectroscopic techniques have great potential as routine methods in clinical microbiology.

Gold standard (test)

10.1128/jcm.39.5.1763-1770.2001

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Citations (265)

A quantitative-PCR protocol rapidly detects αGAL deletions/duplications in patients with Anderson–Fabry disease

Molecular Genetics and Metabolism (2012)

Nicola Marziliano Nadia Sapere Francesco Orsini Valentina Motta Silvio Veronese

10.1016/j.ymgme.2012.01.012

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Citations (4)

Comparison between gamma and beta irradiation effects on hydroxypropylmethylcellulose and gelatin hard capsules

AAPS PharmSciTech (2005)

Francesco Cilurzo Francesca Selmin Paola Minghetti L. Montanari Cristina Lenardi

Gelatin

BETA (programming language)

Gamma Irradiation

10.1208/pt060473

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Citations (17)

Atomic force microscopy imaging of Xenopus laevis oocyte plasma membrane purified by ultracentrifugation

Microscopy Research and Technique (2008)

M. Santacroce Francesco Orsini Stefania A. Mari Massimo Marinone Cristina Lenardi

Atomic force microscopy (AFM) was used to investigate the native plasma membrane of Xenopus laevis (X. laevis) oocyte purified by means of ultracentrifugation on sucrose gradient and subsequently adsorbed on mica leaves through a physisorption process. Reproducible AFM topography images were collected, analyzed, and compared. AFM images showed the presence of large single or double bilayer membrane sheets covered with protein complexes. The lateral dimension and height of protein complexes imaged in air showed a normal distribution centred on 15.4 +/- 0.4 nm (mean +/- SE; n = 59) and 3.9 +/- 0.2 nm (mean +/- SE; n = 57), respectively. A density of about 270 protein complexes per square micron was calculated. Less frequently, ordered nanometer domains with densely packed protein complexes arranged in hexagonal patterns were also visualized in AFM images, confirming previously published data. Their lateral dimension and height showed a normal distribution centred on 23.0 +/- 0.4 nm (mean +/- SE; n = 42) and 1.5 +/- 0.6 nm (mean +/- SE; n = 90), respectively. A density of about 870 protein complexes per square micrometer was calculated. Advantages and drawbacks of this new sample preparation for AFM imaging are discussed.

10.1002/jemt.20559

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Citations (7)