Vandana Ghormade

Agharkar Research Institute

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Mukund V. Deshpande

National Chemical Laboratory

Ejaj K. Pathan

Symbiosis International University

Kishore M. Paknikar

Indian Institute of Technology Bombay

Henry Kolge

Agharkar Research Institute

Gokul Patil

University of Iowa

Santosh G. Tupe

National Chemical Laboratory

Shraddha Rahi

Agharkar Research Institute

Girish Kulkarni

Torrent Pharma (India)

Sachin Jadhav

Agharkar Research Institute

Vikram Lanjekar

Agharkar Research Institute

Cooperative Institutions

National Chemical Laboratory

Agharkar Research Institute

Savitribai Phule Pune University

Academy of Scientific and Innovative Research

Indian Institute of Technology Bombay

National Centre for Cell Science

Council of Scientific and Industrial Research

Bombay College of Pharmacy

Post Graduate Institute of Medical Education and Research

Universidad Nacional Autónoma de México

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Comparison ofMetarhiziumisolates for biocontrol ofHelicoverpa armigera(Lepidoptera: Noctuidae) in chickpea

Biocontrol Science and Technology (2008)

Shuklangi A. Kulkarni Vandana Ghormade Girish Kulkarni Manisha Kapoor Santosh B. Chavan

Abstract Metarhizium isolates from soil (53) and insect hosts (10) were evaluated for extracellular production of cuticle degrading enzyme (CDE) activities such as chitinase, chitin deacetylase (CDA), chitosanase, protease and lipase. Regression analysis demonstrated the relation of CDE activities with Helicoverpa armigera mortality. On basis of this relation, ten isolates were selected for further evaluation. Subsequently, based on LT50 of the 10 isolates towards H. armigera, five isolates were selected. Out of these five isolates, three were selected on the basis of higher conidia production (60–75 g/kg rice), faster sedimentation time (ST50) (2.3–2.65 h in 0.1% (w/v) Tween 80) and lower LC50 (1.4–5.7×103 conidia/mL) against H. armigera. Finally, three Metarhizium isolates were selected for the molecular fingerprinting using ITS sequencing and RAPD patterning. All three isolates, M34412, M34311 and M81123, showed comparable RAPD patterns with a 935G primer. These were further evaluated for their field performance against H. armigera in a chickpea crop. The percent efficacies with the three Metarhizium isolates were from 65 to 72%, which was comparable to the chemical insecticide, endosulfan (74%).

Metarhizium

Biopesticide

Chitinase

Helicoverpa

Beauveria

10.1080/09583150802366475

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Citations (33)

Development of erythromycin loaded PLGA nanoparticles for improved drug efficacy and sustained release against bacterial infections and biofilm formation

Microbial Pathogenesis (2024)

Kamal Mayattu Jyutika M. Rajwade Vandana Ghormade

PLGA

10.1016/j.micpath.2024.107083

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Citations (0)

Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium

Colloids and Surfaces B Biointerfaces (2020)

Gokul Patil Arun Torris P. R. Suresha Sachin Jadhav Manohar V. Badiger

Gelatin

Biocompatibility

Clotting time

10.1016/j.colsurfb.2020.111454

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Citations (24)

Isolation and Characterization of Chitosans from Different Fungi with Special Emphasis on Zygomycetous Dimorphic Fungus Benjaminiella poitrasii: Evaluation of Its Chitosan Nanoparticles for the Inhibition of Human Pathogenic Fungi

Biomacromolecules (2022)

Shamala Mane Ejaj K. Pathan Santosh G. Tupe Sneha Deshmukh Deepika Kale

The cell wall chitosan was extracted from fungi belonging to different taxonomic classes, namely, Benjaminiella poitrasii (Zygomycetes, dimorphic), Hanseniaspora guilliermondii, Issatchenkia orientalis, Pichia membranifaciens, and Saccharomyces cerevisiae (Ascomycetes, yeasts), and Agaricus bisporus and Pleurotus sajor-caju (Basidiomycetes). The maximum yield of chitosan was 60.89 ± 2.30 mg/g of dry mycelial biomass of B. poitrasii. The degree of deacetylation (DDA) of chitosan extracted from different fungi, as observed with 1H NMR, was in the range of 70–93%. B. poitrasii chitosan exhibited the highest DDA (92.78%). The characteristic absorption bands were observed at 3450, 1650, 1420, 1320, and 1035 cm–1 by FTIR. Compared to chitosan from marine sources (molecular weight, MW, 585 kDa), fungal chitosans showed lower MW (6.21–46.33 kDa). Further, to improve the efficacy of B. poitrasii chitosan (Bp), nanoparticles (Np) were synthesized using the ionic gelation method and characterized by dynamic light scattering (DLS). For yeast and hyphal chitosan nanoparticles (BpYCNp and BpHCNp), the average particle size was <200 nm with polydispersity index of 0.341 ± 0.03 and 0.388 ± 0.002, respectively, and the zeta potential values were 21.64 ± 0.34 and 24.48 ± 1.58 mV, respectively. The B. poitrasii chitosans and their nanoparticles were further evaluated for antifungal activity against human pathogenic Candida albicans ATCC 10231, Candida glabrata NCYC 388, Candida tropicalis ATCC 750, Cryptococcus neoformans ATCC 34664, and Aspergillus niger ATCC 10578. BpHCNps showed lower MIC90 values (0.025–0.4 mg/mL) than the chitosan polymer against the tested human pathogens. The study suggested that nanoformulation of fungal chitosan, which has low molecular weight and high % DDA, is desirable for antifungal applications against human pathogens. Moreover, chitosans as well as their nanoparticles were found to be hemocompatible and are therefore safe for healthcare applications.

10.1021/acs.biomac.1c01248

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Citations (12)

Controlled delivery of nikkomycin by PEG coated PLGA nanoparticles inhibits chitin synthase to prevent growth of Aspergillus flavus and Aspergillus fumigatus

Zeitschrift für Naturforschung C (2024)

Kamal Mayattu Vandana Ghormade

Aspergillosis is one of the most common fungal infections that can threaten individuals with immune compromised condition. Due to the increasing resistance of pathogens to the existing antifungal drugs, it is difficult to tackle such disease conditions. Whereas, nikkomycin is an emerging safe and effective antifungal drug which causes fungal cell wall disruption by inhibiting chitin synthase. Hence, the study aims at the development of nikkomycin loaded PEG coated PLGA nanoparticles for its increased antifungal efficiency and inhibiting

Antifungal drugs

10.1515/znc-2023-0185

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Citations (1)

Fungal spore germination into yeast or mycelium: possible implications of dimorphism in evolution and human pathogenesis

The Science of Nature (2000)

Vandana Ghormade Mukund V. Deshpande

Sexual dimorphism

Dimorphic fungus

Spore germination

Budding

10.1007/s001140050711

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Citations (21)

A pH-tuned chitosan-PLGA nanocarrier for fluconazole delivery reduces toxicity and improves efficacy against resistant Candida

International Journal of Biological Macromolecules (2022)

Henry Kolge Gokul Patil Sachin Jadhav Vandana Ghormade

Nanocarriers

PLGA

Candida auris

Antifungal drugs

Efflux

10.1016/j.ijbiomac.2022.12.139

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Citations (11)

PCR–RFLP analysis of chitinase genes enables efficient genotyping of Metarhizium anisopliae var. anisopliae

Journal of Invertebrate Pathology (2009)

J. Enkerli Vandana Ghormade Catherine Oulevey Franco Widmer

Chitinase

Metarhizium

10.1016/j.jip.2009.08.006

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Citations (14)

Selection of reference genes for quantitative real-time RT-PCR assays in different morphological forms of dimorphic zygomycetous fungus Benjaminiella poitrasii

PLoS ONE (2017)

Ejaj K. Pathan Vandana Ghormade Mukund V. Deshpande

Benjaminiella poitrasii, a dimorphic non-pathogenic zygomycetous fungus, exhibits a morphological yeast (Y) to hypha (H) reversible transition in the vegetative phase, sporangiospores (S) in the asexual phase and zygospores (Z) in the sexual phase. To study the gene expression across these diverse morphological forms, suitable reference genes are required. In the present study, 13 genes viz. ACT, 18S rRNA, eEF1α, eEF-Tu,eIF-1A, Tub-α, Tub-b, Ubc, GAPDH, Try, WS-21, NADGDH and NADPGDH were evaluated for their potential as a reference, particularly for studying gene expression during the Y-H reversible transition and also for other asexual and sexual life stages of B. poitrasii. Analysis of RT-qPCR data using geNorm, normFinder and BestKeeper software revealed that genes such as Ubc, 18S rRNA and WS-21 were expressed at constant levels in each given subset of RNA samples from all the morphological phases of B. poitrasii. Therefore, these reference genes can be used to elucidate the role of morpho-genes in B. poitrasii. Further, use of the two most stably expressed genes (Ubc and WS-21) to normalize the expression of the ornithine decarboxylase gene (Bpodc) in different morphological forms of B. poitrasii, generated more reliable results, indicating that our selection of reference genes was appropriate.

Reference Genes

Housekeeping gene

Dimorphic fungus

10.1371/journal.pone.0179454

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Citations (15)

Novel derivatives of arabinogalactan, pullulan & lactobionic acid for targeting asialoglycoprotein receptor: Biomolecular interaction, synthesis & evaluation

International Journal of Biological Macromolecules (2022)

Deepa U. Warrier Anantha Krishnan Dhanabalan K. Gunasekaran Henry Kolge Vandana Ghormade

Asialoglycoprotein receptor

Nanocarriers

Pullulan