Vincent Castric

Unité Évolution, Écologie et Paléontologie

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Xavier Vekemans

Centre National de la Recherche Scientifique

Sylvain Billiard

Centre National de la Recherche Scientifique

Mathieu Genête

Centre National de la Recherche Scientifique

ÉD

Éléonore Durand

Université de Lille

Céline Poux

Centre National de la Recherche Scientifique

Audrey Le Vève

Unité Évolution, Écologie et Paléontologie

Sophie Gallina

Centre National de la Recherche Scientifique

Pierre Saumitou‐Laprade

Université de Lille

Chloé Ponitzki

Unité Évolution, Écologie et Paléontologie

William Marande

Laboratoire de Recherche en Sciences Végétales

Cooperative Institutions

Centre National de la Recherche Scientifique

132

Université de Lille

Unité Évolution, Écologie et Paléontologie

Laboratoire de Génétique & Evolution des Populations Végétales

Université Lille Nord de France

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Université Paris-Saclay

Institut National de la Recherche Agronomique

Université de Montpellier

Inserm

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

eLife assessment: Improved inference of population histories by integrating genomic and epigenomic data

Vincent Castric

Epigenomics

10.7554/elife.89470.4.sa0

Cite

Citations (0)

Bulk pollen sequencing reveals rapid evolution of segregation distortion in the male germline of Arabidopsis hybrids

Evolution Letters (2019)

Russell Corbett‐Detig Paloma Medina Hélène Frérot Christelle Blassiau Vincent Castric

Genes that do not segregate in heterozygotes at Mendelian ratios are a potentially important evolutionary force in natural populations. Although the impacts of segregation distortion are widely appreciated, we have little quantitative understanding about how often these loci arise and fix within lineages. Here, we develop a statistical approach for detecting segregation distorting genes from the comprehensive comparison of whole genome sequence data obtained from bulk gamete versus somatic tissues. Our approach enables estimation of map positions and confidence intervals, and quantification of effect sizes of segregation distorters. We apply our method to the pollen of two interspecific F1 hybrids of Arabidopsis lyrata and A. halleri and we identify three loci across eight chromosomes showing significant evidence of segregation distortion in both pollen samples. Based on this, we estimate that novel segregation distortion elements evolve and achieve high frequencies within lineages at a rate of approximately one per 244,000 years. Furthermore, we estimate that haploid-acting segregation distortion may contribute between 10% and 30% of reduced pollen viability in F1 individuals. Our results indicate haploid acting factors evolve rapidly and dramatically influence segregation in F1 hybrid individuals.

Mendelian inheritance

Gamete

10.1002/evl3.96

Cite

Citations (21)

Contrasted Patterns of Molecular Evolution in Dominant and Recessive Self-Incompatibility Haplotypes in Arabidopsis

PLoS Genetics (2012)

Pauline M. Goubet Hélène Bergès Arnaud Bellec Elisa Prat Nicolas Helmstetter

Self-incompatibility has been considered by geneticists a model system for reproductive biology and balancing selection, but our understanding of the genetic basis and evolution of this molecular lock-and-key system has remained limited by the extreme level of sequence divergence among haplotypes, resulting in a lack of appropriate genomic sequences. In this study, we report and analyze the full sequence of eleven distinct haplotypes of the self-incompatibility locus (S-locus) in two closely related Arabidopsis species, obtained from individual BAC libraries. We use this extensive dataset to highlight sharply contrasted patterns of molecular evolution of each of the two genes controlling self-incompatibility themselves, as well as of the genomic region surrounding them. We find strong collinearity of the flanking regions among haplotypes on each side of the S-locus together with high levels of sequence similarity. In contrast, the S-locus region itself shows spectacularly deep gene genealogies, high variability in size and gene organization, as well as complete absence of sequence similarity in intergenic sequences and striking accumulation of transposable elements. Of particular interest, we demonstrate that dominant and recessive S-haplotypes experience sharply contrasted patterns of molecular evolution. Indeed, dominant haplotypes exhibit larger size and a much higher density of transposable elements, being matched only by that in the centromere. Overall, these properties highlight that the S-locus presents many striking similarities with other regions involved in the determination of mating-types, such as sex chromosomes in animals or in plants, or the mating-type locus in fungi and green algae.

Molecular evolution

10.1371/journal.pgen.1002495

Cite

Citations (91)

Dominance-récessivité des gènes : le mécanisme élucidé !

Sylvain Billiard Vincent Castric Pascale Mollier

Pourquoi certains genes sont-ils dominants et d’autres recessifs ? Ce phenomene fondamental en biologie a ete eclairci apres presqu’un siecle d’interrogations, d’hypotheses et d’experimentations...

Dominance (genetics)

Source

Cite

Citations (0)

Breakdown of gametophytic self-incompatibility in subdivided populations

bioRxiv (Cold Spring Harbor Laboratory) (2018)

Thomas Brom Vincent Castric Sylvain Billiard

Abstract Many hermaphroditic flowering plants species possess a genetic self-incompatibility (SI) system that prevents self-fertilization and is typically controlled by a single multiallelic locus, the S-locus. The conditions under which SI can be stably maintained in single isolated populations are well known and depend chiefly on the level of inbreeding depression and the number of SI alleles segregating at the S-locus. However, while both the number of SI alleles and the level of inbreeding depression are potentially affected by population subdivision, the conditions for the maintenance of SI in subdivided populations remain to be studied. In this paper, we combine analytical predictions and two different individual-based simulation models to show that population subdivision can severely compromise the maintenance of SI. Under the conditions we explored, this effect is mainly driven by the decrease of the local diversity of SI alleles rather than by a change in the dynamics of inbreeding depression. We discuss the implications of our results for the interpretation of empirical data on the loss of SI in natural populations. Data accessibility statement No data to be archived

Genetic load

10.1101/444125

Cite

Citations (0)

The genetic architecture of the load linked to dominant and recessive self-incompatibility alleles in Arabidopsis halleri and A. lyrata

Audrey Le Vève Mathieu Genête Christelle Lepers-Blassiau Chloé Ponitzki Céline Poux

The long-term balancing selection acting on mating-types or sex determining genes is expected to lead to the accumulation of deleterious mutations in the tightly linked chromosomal segments that are locally “sheltered” from purifying selection. However, the factors determining the extent of this accumulation are poorly understood. Here, we take advantage of the large number of alleles at the sporophytic self-incompatibility system of the Brassicaceae along a complex dominance hierarchy to evaluate how the pace at which linked deleterious mutations accumulate varies with the intensity of balancing selection. We first experimentally measured the phenotypic manifestation of the linked load at three different levels of the dominance hierarchy. We then sequenced and phased polymorphisms in the chromosomal regions linked to 126 distinct copies of S -alleles in two populations of Arabidopsis halleri and three populations of A. lyrata . We find that linkage to the S -locus locally distorts phylogenies over about 10-30kb along the chromosome. The more intense balancing selection on dominant S -alleles results in greater fixation of deleterious mutations, while recessive S -alleles accumulate more linked deleterious mutations that are segregating. Hence, the structure rather than the overall magnitude of the linked genetic load differs between dominant and recessive S -alleles. Our results have consequences for the long-term evolution of new S -alleles, the evolution of dominance modifiers between them, and raise the question of why the non-recombining regions of some sex and mating type chromosomes expand over evolutionary times while others, such as that the S -locus of the Brassicaceae, remain restricted to small chromosomal regions.

Genetic architecture

Genetic load

10.7554/elife.94972.1

Cite

Citations (0)

The genetic architecture of the load linked to dominant and recessive self-incompatibility alleles inArabidopsis halleriandA. lyrata

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Audrey Le Vève Mathieu Genête Christelle Lepers-Blassiau Chloé Ponitzki Céline Poux

Abstract The long-term balancing selection acting on mating-types or sex determining genes is expected to lead to the accumulation of deleterious mutations in the tightly linked chromosomal segments that are locally “sheltered” from purifying selection. However, the factors determining the extent of this accumulation are poorly understood. Here, we took advantage of variations in the intensity of balancing selection along a dominance hierarchy formed by alleles at the sporophytic self-incompatibility system of the Brassicaceae to compare the pace at which linked deleterious mutations accumulate among them. We first experimentally measured the phenotypic manifestation of the linked load at three different levels of the dominance hierarchy. We then sequenced and phased polymorphisms in the chromosomal regions linked to 126 distinct copies of S -alleles in two populations of Arabidopsis halleri and three populations of A. lyrata . We find that linkage to the S -locus locally distorts phylogenies over about 10-30kb along the chromosome. The more intense balancing selection on dominant S -alleles results in greater fixation of linked deleterious mutations, while recessive S -alleles accumulate more linked deleterious mutations that are segregating. Hence, the structure rather than the overall magnitude of the linked genetic load differs between dominant and recessive S -alleles. Our results have consequences for the long-term evolution of new S -alleles, the evolution of dominance modifiers between them, and raise the question of why the non-recombining regions of some sex and mating type chromosomes expand over evolutionary times while others, such as that the S -locus of the Brassicaceae, remain restricted to small chromosomal regions.

Balancing selection

Dominance (genetics)

Genetic load

Genetic architecture

Selfing

10.1101/2023.11.12.566794

Cite

Citations (0)

Molecular Evolution within and between Self-Incompatibility Specificities

Molecular Biology and Evolution (2009)

Vincent Castric Jesper Bechsgaard S. Grenier Rachid Noureddine Mikkel Heide Schierup

Genes under multiallelic balancing selection have sharply contrasted evolutionary dynamics across timescales, with much longer coalescence time among functionally distinct allelic lines but much shorter coalescence time among gene copies within allelic lines as compared with the genomic background. In this paper, we combine theoretical and empirical approaches to investigate patterns of molecular evolution within and between self-incompatibility (SI) specificities. We first use numerical simulations to investigate coalescence times within allelic lines in a subdivided population for a sporophytic SI system. We then report on a comprehensive analysis of nucleotide polymorphism among gene copies within five distinct allelic lines in the closely related Arabidopsis halleri and Arabidopsis lyrata. In line with our model predictions, we find that the observed level of polymorphism among gene copies was generally low but differed among allelic lines. The data provide compelling direct evidence for recombination and/or gene conversion not only within the two most recessive allelic lines but also between two closely related but distinct allelic lines, suggesting that recombination at the Arabidopsis SI locus is possible in the absence of large sequence divergence among haplotypes. We observed shared polymorphic sites between the two species in one allelic line and strikingly similar haplotypes in another allelic line. We discuss whether convergent evolution may have led to this pattern and suggest that these observations are consistent with ongoing or very recent introgression, as previously documented.

Balancing selection

Introgression

Gene conversion

10.1093/molbev/msp224

Cite

Citations (43)

Effect of balancing selection on spatial genetic structure within populations: theoretical investigations on the self-incompatibility locus and empirical studies in Arabidopsis halleri

Heredity (2010)

J-B Leducq Violaine Llaurens Vincent Castric Pierre Saumitou‐Laprade Olivier J. Hardy

Balancing selection

Directional selection

genetic model

10.1038/hdy.2010.68

Cite

Citations (30)

eLife assessment: Positive selection and relaxed purifying selection contribute to rapid evolution of male-biased genes in a dioecious flowering plant

Vincent Castric

Sex-biased genes offer insights into the evolution of sexual dimorphism. Sex-biased genes, especially those with male bias, show elevated evolutionary rates of protein sequences driving by positive selection and relaxed purifying selection in animals. Although rapid sequence evolution of sex-biased genes and evolutionary forces have been investigated in animals and brown algas, less is known about them in dioecious angiosperms. In this study, we separately compared the expression of sex-biased gene between female and male flower buds and between female and male mature flowers in dioecious Trichosanthes pilosa (Cucurbitaceae). In floral buds, sex-biased gene expression was pervasive, and had significantly different roles on sexual dimorphism such as physiology. We observed higher rates of sequence evolution for male-biased genes in floral buds compared to female-biased and unbiased genes. Male-biased genes under positive selection were mainly related to abiotic and biotic stress, suggesting that high evolutionary rates are driven by adaptive evolution. Additionally, relaxed purifying selection may contribute to accelerated evolution in male-biased genes generated by gene duplication. Our findings, for the first time in dioecious angiosperms, suggest evident rapid evolution of male-biased genes, advance our understanding of the patterns and forces driving the evolution of sexual dimorphism in dioecious plants.

Sexual dimorphism

Negative selection

Experimental Evolution

10.7554/elife.89941.1.sa3

Cite

Citations (0)