José Miguel Soriano

Universitat de Lleida

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M. L. Badenes

Instituto Valenciano de Investigaciones Agrarias

G. Llácer

Instituto Valenciano de Investigaciones Agrarias

C. Royo

Institute for Research and Technology in Food and Agriculture

Carlos Romero

Universitat Politècnica de València

Santiago Vilanova

Universitat Politècnica de València

Marta S. Lopes

Institute for Research and Technology in Food and Agriculture

Rubén Rufo

Institute for Research and Technology in Food and Agriculture

D. Villegas

Institute for Research and Technology in Food and Agriculture

ÁG

Ágata Gadaleta

University of Bari Aldo Moro

Henk J. Schouten

Wageningen University & Research

Cooperative Institutions

Wageningen University & Research

Consejo Superior de Investigaciones Científicas

Instituto Valenciano de Investigaciones Agrarias

Institute for Research and Technology in Food and Agriculture

Institute of Field and Vegetable Crops

University of Bologna

University of Novi Sad

Graduate School Experimental Plant Sciences

Swedish University of Agricultural Sciences

Instituto de Agricultura Sostenible

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CISGENESIS IS A PROMISING APPROACH FOR FAST, ACCEPTABLE AND SAFE BREEDING OF PIP FRUIT

Acta Horticulturae (2009)

Henk J. Schouten José Miguel Soriano Sameer Joshi Anne Kortstee Frans A. Krens

Introgression of traits from wild germplasm into pip fruit cultivars by means of classical breeding is painstakingly slow. Introgression of e.g., the apple scab resistance gene Vf from Malus floribunda 821 into marketable high quality apple cultivars took approximately 50 years. In the mean time the Vf resistance is being broken down in Europe. For durable resistance, different resistance genes should be accumulated. However, this may take another series of decades. This slow tempo is caused mainly by the long juvenile period of apple and the phenomenon that not only the allele of interest is inherited by the progeny, but also hundreds of unwanted alleles. The process would be much faster if only the allele of interest was inserted, without unwanted alleles. This can be achieved by cisgenesis. We defined cisgenesis as genetic modification of plants, inserting alleles of the plant itself or from crossable relatives. The allele should contain its native introns and should be flanked by its native promoter and terminator in sense-orientation. If the plant is equipped with foreign genes from outside the gene pool of the conventional breeder, the plant is named transgenic. Inquiries indicate that cisgenic plants are more acceptable to consumers than transgenic plants. As the phenotypic result of cisgenesis can, in principle, also be obtained by means of conventional breeding or translocation breeding, cisgenic plants are as safe as plants from conventional breeding or mutation breeding. Therefore we have proposed to treat cisgenic plants like conventionally bred plants, by exempting cisgenic plants from the GMO regulation. The number of isolated, functionally analysed genes and their alleles from fruit tree crops is increasing. Also technologies are available for introduction of these alleles without leaving selection genes behind. Cisgenesis is combining the knowledge of native alleles with marker free technologies. Cisgenesis is a promising path for utilizing the wealth of knowledge on plant genes to the benefit of the society in a fast, safe, and acceptable way.

Introgression

Germ plasm

Plant Breeding

10.17660/actahortic.2009.814.26

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Citations (10)

Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region

Molecular Plant Pathology (2011)

ELSA MARÍA VERA RUIZ José Miguel Soriano Carlos Romero Tetyana Zhebentyayeva Javier Terol

SUMMARY Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot ( Prunus armeniaca L.), and most studies have located a major PPV resistance locus ( PPVres ) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high‐density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV‐resistant cultivars, respectively. Using these maps, and excluding genotype–phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40 cm encompassing PPVres . Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high‐information content fingerprinting (HICF). In addition, a total of 300 BAC‐derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified.

Synteny

Prunus armeniaca

Bacterial artificial chromosome

10.1111/j.1364-3703.2010.00691.x

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Citations (34)

Are the agronomic performance and grain quality characteristics of bread wheat Mediterranean landraces related to the climate prevalent in their area of origin?

Journal of Cereal Science (2022)

C. Royo José Miguel Soriano Rubén Rufo Carlos Guzmán

This study analyses the relationship between the climate of the countries where wheat landraces are specifically adapted, and their agronomic and grain quality characteristics. Regions with similar climate within the Mediterranean Basin were identified based on long-term climatic data of the 23 countries origin of a collection of 153 bread wheat landraces. The panel was genotyped with 13177 SNP markers and was grown on field experiments for two years under rainfed conditions in north-east Spain and 14 agronomic and 11 grain-quality traits were assessed. Great phenotypic variability was found in the collection. The agronomic performance of the landraces varied according to the climate of the four climatic regions identified within the Mediterranean Basin (south-east, south-west, north coast and north-Balkan), which gradually varies from warm and dry to wet and cold. Cycle length, grain-filling rate and yield increased, but grain-filling duration decreased from south-eastern landraces to north Balkan ones. Grain weight accounted for 25% of yield variations and was lowest in landraces from the south-eastern region. Grain quality showed no geographical pattern related to the climatic region. Landraces from Bosnia-Herzegovina, Bulgaria and Romania differed from the rest in their high gluten strength (W), loaf volume (LV), mixing time (MT) and grain hardness, while opposite attributes were found in accessions from Jordan, Lebanon and Cyprus. Landraces from south-western Mediterranean countries had low MT, alveograph-peak, W and LV. Genotypes suitable for use in breeding programmes may be identified. Molecular analyses revealed that genetic structure was mostly influenced by high temperatures before anthesis and rainfall, solar radiation and sunshine after anthesis.

Mediterranean Basin

Grain Quality

Germ plasm

10.1016/j.jcs.2022.103478

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Citations (12)

CLONING AND MAPPING OF RESISTANCE GENE HOMOLOGUES IN APRICOT (PRUNUS ARMENIACA L.)

Acta Horticulturae (2006)

José Miguel Soriano C. Romero Santiago Vilanova G. LJacer M. L. Badenes

Prunus armeniaca

Cloning (programming)

10.17660/actahortic.2006.717.22

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Citations (0)

GENETIC TOOLS FOR SELECTING RESISTANCE TO SHARKA DISEASE IN APRICOT

Acta Horticulturae (2012)

José Miguel Soriano M.L. Domigo Elena Zuriaga C. Romero G. Llácer

10.17660/actahortic.2012.966.40

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Citations (2)

A consensus map for quality traits in durum wheat based on genome-wide association studies and detection of ortho-meta QTL across cereal species

Frontiers in Genetics (2022)

Ilaria Marcotuli José Miguel Soriano Ágata Gadaleta

The present work focused on the identification of durum wheat QTL hotspots from a collection of genome-wide association studies, for quality traits, such as grain protein content and composition, yellow color, fiber, grain microelement content (iron, magnesium, potassium, selenium, sulfur, calcium, cadmium), kernel vitreousness, semolina, and dough quality test. For the first time a total of 10 GWAS studies, comprising 395 marker-trait associations (MTA) on 57 quality traits, with more than 1,500 genotypes from 9 association panels, were used to investigate consensus QTL hotspots representative of a wide durum wheat genetic variation. MTA were found distributed on all the A and B genomes chromosomes with minimum number of MTA observed on chromosome 5B (15) and a maximum of 45 on chromosome 7A, with an average of 28 MTA per chromosome. The MTA were equally distributed on A (48%) and B (52%) genomes and allowed the identification of 94 QTL hotspots. Synteny maps for QTL were also performed in

Genome-wide Association Study

Candidate gene

Synteny

Brachypodium

Grain Quality

Genetic Association

Family-based QTL mapping

Association mapping

Chromosome regions

10.3389/fgene.2022.982418

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Citations (9)

RESISTANCE TO PLUM POX VIRUS: A MOLECULAR APPROACH

Acta Horticulturae (2004)

Santiago Vilanova Carlos Romero José Miguel Soriano J. Martínez-Calvo G. Llácer

Pox virus

10.17660/actahortic.2004.657.42

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Citations (1)

Pasta-Making Quality QTLome From Mediterranean Durum Wheat Landraces

Frontiers in Plant Science (2018)

Martina Roselló C. Royo Fanny Álvaro D. Villegas Ruyman Nazco

In order to identify genome regions related to pasta-making quality traits, association mapping (AM) was performed in a set of 165 durum wheat landraces from 21 Mediterranean countries. The collection was genotyped using 1149 DArT markers and 872 of them with a known genetic position were used for AM. The collection was grown in north-east Spain during 3 years. Results of ANOVA showed that trait variation for quality traits, except for grain protein content (GPC), was mainly explained by genetic effects. Landraces showed higher GPC than modern cultivars but lower gluten strength (GS). Modern and eastern landraces showed the highest yellow color index (YI). Balkan landraces showed the lowest test weight (TW). A total of 92 marker-trait associations were detected, 20 corresponding to GS, 21 to GPC, 21 to YI and 30 to TW. With the aim of detecting new genomic regions involved in grain quality, the position of the associations was compared with previously mapped QTL by a meta-QTL analysis. A total of 249 QTLs were projected onto the same map used for AM, identifying 45 meta-QTL (MQTL) regions and the remaining 15 QTLs as singletons. The position of known genes involved in grain quality was also included, and gene annotation within the most significant regions detected by AM was carried out using the wheat genome sequence.

10.3389/fpls.2018.01512

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Citations (31)

Development of microsatellite markers in polyploid persimmon (Diospyros kaki Lf) from an enriched genomic library

Molecular Ecology Notes (2006)

José Miguel Soriano Simona Pecchioli Carlos Romero Santiago Vilanova G. Llácer

Abstract The oriental persimmon ( Diospyros kaki Lf) is believed to have originated in China with subsequent introduction into Japan and Korea in ancient times. The species was then brought to Europe, Brazil and the USA from Japan in the 19th century. Recent studies highlighted the poor state of identification of cultivars in these countries due to incorrect labelling and presence of synonyms among local varieties. Thus, molecular marker characterization of germplasm resources is of great value for genetic resource preservation and plant breeding of persimmon. Therefore, to identify accessions for further plant breeding and germplasm management, 37 microsatellite loci were developed from a CT/AG‐enriched persimmon genomic library.

Diospyros kaki

Germ plasm

Polyploid

Diospyros

10.1111/j.1471-8286.2006.01236.x

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Citations (39)

Unravelling consensus genomic regions conferring leaf rust resistance in wheat via meta-QTL analysis

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Aduragbemi Amo José Miguel Soriano

Abstract Leaf rust, caused by the fungus Puccinia triticina Erikss (Pt), is a destructive disease affecting wheat and a threat to food security. Developing resistant varieties represents a useful method of disease control, and thus, understanding the genetic basis for leaf rust resistance is required. To this end, a comprehensive bibliographic search for leaf rust resistance quantitative trait loci (QTLs) was performed, and 393 QTLs were collected from 50 QTL mapping studies. Afterwards, a consensus map with a total length of 4567 cM consisting of different types of markers (SSR, DArT, Chip-based SNP markers and SNP markers from GBS) was used for QTL projection, and meta-QTL analysis was performed on 320 QTLs. A total of 75 genetic map positions (gmQTLs) were discovered and refined to 15 high confidence mQTLs (hcmQTLs). The candidate genes discovered within the hcmQTL interval were then checked for differential expression using data from three transcriptome studies, resulting in 92 differentially expressed genes (DEGs). The expression of these genes in various leaf tissues during wheat development was explored. This study provides insight into leaf rust resistance in wheat and thereby provides an avenue for developing resistant varieties by incorporating the most important hcmQTLs.

Rust (programming language)

Candidate gene

Family-based QTL mapping

Wheat leaf rust

10.1101/2021.05.11.443557

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Citations (13)