Guoao Gong

Wuhan University of Science and Technology

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Yingying Gu

State Key Laboratory of Respiratory Disease

Yong Mei

Wuhan University of Science and Technology

Jiangyang Qin

Wuhan University of Science and Technology

Yuting Jiang

Anhui Normal University

Daoting Deng

Wuhan University of Science and Technology

Lixia Luo

Wuhan University of Science and Technology

Dandan Wang

Anhui Xinhua University

Xiong Cheng

Lanzhou University

Yong Mei

Wuhan University of Science and Technology

Lixia Luo

Sun Yat-sen University

Cooperative Institutions

Wuhan University of Science and Technology

Chinese Academy of Sciences

Shenzhen Bao'an District People's Hospital

Lanzhou University

Wuhan University

Academy of Military Medical Sciences

Soochow University

Wuhan University of Technology

Sun Yat-sen University

University of Shanghai for Science and Technology

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Electrochemical Immunosensor for the Determination of Nuclear Matrix Protein 22 in Urine Using Carboxylated MWCNTs and Zeolitic Imidazolate Framework Modified with AgNPs

ACS Applied Nano Materials (2023)

Wang DanDan Lixia Luo Daoting Deng Guoao Gong Jiangyang Qin

Nuclear matrix protein 22 (NMP22) is considered one of the noninvasive biological indications in the urine that has recently captured extensive clinical attention for bladder cancer screening and surveillance. Hence, it is imperative to create a straightforward, sensitive, and precise NMP22 assay. As a type of carbon nanomaterial, carboxylated multiwalled carbon nanotubes (CMWCNTs) exhibit exceptional electrical conductivity and abundant carboxyl groups. The silver nanoparticles and zeolitic imidazolate frameworks-8 composite (AgNPs-ZIF-8), which is a three-dimensional material, possess a wide surface area, acceptable electrical conductivity, and satisfactory biocompatibility. More importantly, it can also immobilize NMP22 antibodies in striking amounts via the Ag–NH2 bonds. Along these lines, in this work, AgNPs were decorated on ZIF-8 and modified on the glassy carbon electrode together with CMWCNTs. The comparative advantages of carbon nanotubes, metal–organic frameworks, and AgNPs were successfully combined to develop an electrochemical immunosensor with superior sensitivity and reliability for the detection of NMP22 in urine. It should be mentioned that the immunosensor not only has a wide linear range (10–2 to 105 pg mL–1) and a low detection limit (8.8 fg mL–1, S/N = 3) but also exhibits robust precision, excellent selectivity, and desirable stability. Aside from that, the immunosensor was effectively utilized to detect human urine samples, demonstrating an excellent performance.

Imidazolate

Zeolitic imidazolate framework

Nanomaterials

Biocompatibility

Matrix (chemical analysis)

Silver nanoparticle

Linear range

10.1021/acsanm.3c03603

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Citations (4)

Electrochemical Immunosensor for Nuclear Matrix Protein 22 Determination in Urine Using Carboxylated MWCNTs and Zeolitic Imidazolate Framework Modified with AgNPs

Dandan Wang Lixia Luo Daoting Deng Guoao Gong Jiangyang Qin

Bladder cancer is a highly prevalent and frequently occurring tumor. Nuclear matrix protein 22 (NMP22) is one of the non-invasive biological indications in the urine that has captured extensive clinical attention recently for bladder cancer screening and surveillance. Nevertheless, at the pre-tumor stage, the universally used clinical immunoassays are unable to identified trace amounts of NMP22 in urine. Hence, it is imperative to create a straightforward, sensitive, and precise NMP22 assay. As a type of carbon nanomaterial, carboxylated multi-walled carbon nanotubes exhibit exceptional electrical conductivity and abundant carboxyl groups. The silver nanoparticles and zeolitic imidazolate frameworks-8 (AgNPs@ZIF-8) composite, which is a three-dimensional material, has a wide surface area, acceptable electrical conductivity, and satisfactory biocompatibility. More importantly, it can also immobilize NMP22 antibodies in striking amounts via Ag-NH2 bonds. The benefits of carbon nanotubes, metal-organic frameworks, and AgNPs were successfully combined to develop an electrochemical immunosensor with superior sensitivity and reliability to detect NMP22 in urine. It deserves to be mentioned that the immunosensor not only has a wide linear range (10-2pg mL-1~105pg mL-1) and a low detection limit (8.8 fg mL-1, S/N = 3), but exhibits robust repeatability, excellent selectivity, and desirable stability. Aside from that, the immunosensor was effectively utilized to detect human urine samples, which showed excellent performance.

Imidazolate

Zeolitic imidazolate framework

Matrix (chemical analysis)

10.2139/ssrn.4502301

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Electrochemical Immunosensor Modified with Nitrogen-Doped Reduced Graphene Oxide@Carboxylated Multi-Walled Carbon Nanotubes/Chitosan@Gold Nanoparticles for CA125 Detection

Chemosensors (2022)

Yingying Gu Guoao Gong Yuting Jiang Jiangyang Qin Yong Mei

Lung cancer is one of the malignant tumors with the highest mortality rate, and the detection of its tumor marker carcinoma antigen 125 (CA125) is significant. Here, an electrochemical immunoassay for CA125 was described. Nitrogen-doped reduced graphene oxide (N-rGO), carboxylated multi-walled carbon nanotubes (CMWCNTs) and gold nanoparticles (AuNPs) were applied to co-modify glassy carbon electrode (GCE), after incubation with Anti-CA125, the modified electrode was employed for the specific detection of CA125. The N-rGO@CMWCNTs (Nitrogen-doped reduced graphene oxide@carboxylated multi-walled carbon nanotubes) were used as a matrix, while CS@AuNPs (Chitosan@gold nanoparticles) with high conductivity and biocompatibility was immobilized on it through the reaction between carboxyl groups from CMWCNTs and amino groups, hydroxyl groups from chitosan (CS), resulting in the effect of double signal amplification. The immunosensor demonstrated excellent electrochemical performance with a linear detection range of 0.1 pg mL−1–100 ng mL−1, and the detection limit was as low as 0.04 pg mL−1 (S/N = 3). It had been verified that this method had good precision and high accuracy, and the immunosensor could remain stable for 10 days. This research provided a new method for the detection of CA125 in serum.

Biocompatibility

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10.3390/chemosensors10070272

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Citations (13)

Detection of CYFRA21-1 in serum by electrochemical immunosensor based on nanocomposite consisting of AuNPs@CMK-3@CMWCNTs

Bioelectrochemistry (2022)

Yingying Gu Yuting Jiang Guoao Gong Xiong Cheng Yong Mei

Nanomaterials

Linear range

10.1016/j.bioelechem.2022.108230

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Citations (11)