J García-Tamayo

Hospital Universitario de Maracaibo

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Eduardo Blasco-Olaetxea

Canary Institute of Cancer Research

Julia Molina

Hospital Universitario de Gran Canaria Doctor Negrín

David Parada

Institut d'Investigació Sanitària Pere Virgili

José Esparza

University of Maryland, Baltimore

Eduardo Caleiras

Spanish National Cancer Research Centre

Luzardo-Baptista Mj

A Avila-Mayor

R B José Sardi

Hospital Clínico Universitario de Caracas

Mirian Naranjo de Gómez

Central University of Venezuela

Alexis Rodrı́guez-Acosta

Central University of Venezuela

Cooperative Institutions

Central University of Venezuela

University of Zulia

Hospital Clínico Universitario de Caracas

Canary Institute of Cancer Research

Instituto de Medicina Tropical

Hospital Clínico San Carlos

Hospital Vargas

Hospital Universitario de Maracaibo

Bolivarian University of Venezuela

Instituto Venezolano de Investigaciones Científicas

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Fine anatomy of neurosecretory cells in the human gingiva

Oral Surgery Oral Medicine Oral Pathology (1974)

Mario J. Luzardo-Baptista J García-Tamayo

10.1016/0030-4220(74)90346-6

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Citations (5)

Human genital candidiasis: histochemistry, scanning and transmission electron microscopy.

PubMed (1982)

J García-Tamayo Graciela del Valle Castillo Martínez Aj

Exudates from the vagina and uterine cervix of ten patients suffering from genital candidiasis (Candida albicans) were studied with transmission and scanning electron microscopy and with histochemical techniques in order to find the precise location of the Candida infection and to examine the relationship between the vaginal and cervical cells and the infecting yeasts. It was found that Candida species can penetrate, invade, develop and proliferate within the deep layers of intact cells of the cervix and vaginal mucosal epithelium. The presence of mucopolysaccharides in the glycoproteinaceous coat of blastospores and pseudohyphae was demonstrated both outside and within squamous epithelial cells. The importance of the integrity of the glycoprotein coat during the pathogenesis of human infection with C. albicans is therefore emphasized. Furthermore, the histoinvasive yeast apparently produced cytolytic enzymes during its growth within the human vaginal and cervical mucosal epithelium. The intracellular growth of Candida organisms may represent a protective mechanism of the fungus against the host and a manner of resistance to antimycotic therapy.

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Adrenal gland ultrastructural pathology in plasmodium berghei parasitized mice

Proceedings annual meeting Electron Microscopy Society of America (1996)

M Pulido-Méndez H Finol Alexis Rodrı́guez-Acosta A Márquez I Aguilar

The use of animal models is widely accepted in malaria research. Plasmodium berghei has been extensively studied because it produces a mortal malaria in rodents. Electron microscopic studies on brain and liver pathology have been performed in this infection. In a previous ultrastructural work we reported the capillary alterations observed in adrenal cortex in P. berghei infected mice. In this work we describe the cell and capillary changes we found in adrenal cortex and medulla in this infection. Male mice weighing 18-22 g were inoculated intraperitoneally with P. berghei infected erythrocytes. At the ninth day animals were sacrificed when parasitemia ranged between 80-90%. Adrenal gland samples were processed by routine techniques and observed in a Hitachi H-500 transmission electron microscope.

Plasmodium berghei

Medulla

10.1017/s0424820100166208

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Diagnostic Immunohistochemistry in ovarian metastases.

J García-Tamayo Eduardo Blasco-Olaetxea Julia Molina Vanessa Marques Ferreira Méndez María Teresa Sabatino

In order to analyse the importance of immunohistochemistry for the diagnosis and treatment of ovarian metastasic tumors, 18 cases were selected. A variety of antibodies were selected depending of the histological diagnosis in every case. Among the 18 cases, 7 of them were metastatic tumors from de gastrointestinal tract; in 5 cases, the diagnosis of mucinous adenocarcinoma of de colon was made, and 2 of them were originated from the stomach or pancreas. In 6 cases the primary tumor was in the opposite ovary with extension to the epiplon or the peritoneal surface. In 5 cases, the primary tumor was observed in the mammary glands, in the cervix uteri or the endometrium. Immunohistochemical studies in metastatic tumors to the ovaries were determinant to evaluate the treatment and prognosis of patients.

Ovarian tumor

Metastatic adenocarcinoma

Corpus Uteri

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Superficial bladder cancer: Study of the proliferative nuclear fraction as a prognostic factor

European Journal of Cancer (1996)

Eduardo Blasco-Olaetxea Luis Belloso J García-Tamayo

Immunostaining

Bladder Neoplasm

Epithelioma

10.1016/0959-8049(95)00545-5

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Citations (16)

Placental and fetal alterations due to Venezuelan equine encephalitis virus in rats

Infection and Immunity (1981)

J García-Tamayo José Esparza A. J. Martinez

Histopathological changes in the placentas, embryos, and fetuses of rats inoculated intraperitoneally with the virulent Guajira strain of Venezuelan equine encephalitis virus were studied by light microscopy and immunoperoxidase methods. Rats inoculated before day 15 of pregnancy showed necrosis and hemorrhages in the embryonic disks. Swelling of cytoplasm and nuclear pyknosis of cyto- and syncytotrophoblastic cells were noted as early as 2 days after inoculation. During weeks 1 and 2 of pregnancy, death of the embryos was always observed 3 to 4 days after Venezuelan equine encephalitis virus inoculation. Placental and fetal damage varied among the specimens. In rats 18 days pregnant and sacrificed 2 days after inoculation, there were some viable fetuses; the placentas showed inflammatory reactions in the mesometrial and decidual vessels. Other rats sacrificed at 3 to 4 days after inoculation showed large placental infarcts with fetal death. Viremia peaked during day 2 after inoculation. Immunoperoxidase stains demonstrated viral antigens present in the decidua, myometrium, and cyto- and syncytotrophoblastic cells. These experiments provide additional data regarding the pathogenesis and structural damage in the placental and fetal tissues caused by Venezuelan equine encephalitis virus.

Immunoperoxidase

Decidua

Viremia

Pathogenesis

10.1128/iai.32.2.813-821.1981

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Citations (10)

Venezuelan equine Encephalomyelitis virus: Structural components

Investigación Clínica (1974)

J García-Tamayo Slavia Ryder Elena Ryder

Venezuelan equine encephalomyelitis (VEE) virus, purified in sucrose density gradients was examined with the e1ectron microscope before and after sodium desoxycholate (DOC) treatment. The structure of the nucleocapsid revealed 10 to 12 nm ring shaped units organized into an icosahedral symmetry. Density gradient fractions exhibiting hemagglutinating activity after DOC treatment show 12 to 18 nm particles lined by short projections. These isolated hemagglutinating subunlts are though to correspond to the superficial spikes of VEE virus.

Buoyant density

Density gradient

Encephalomyelitis

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Acid Phosphatase Activity in Mouse Brain Infected with Venezuelan Equine Encephalomyelitis Virus

Journal of Virology (1971)

J García-Tamayo

The mode of development of Venezuelan equine encephalomyelitis virus and the activity of acid phosphatase in the central nervous system of newborn mice were investigated. Precursor particles appeared to be formed in masses of viroplasm, migrating to the membrane of the Golgi cisterns and vacuoles or to the plasma membrane and being transformed into mature viral particles by budding. Mature viral particles were also found in the lumen of the blood vessels and around the myelin sheath of axons. Increased number of Golgi complexes and depletion of polysomes were the main ultrastructural alterations of the nerve cells. Acid phosphatase activity was found to be increased in the Golgi cisterns, vacuoles, and lysosomes of nerve cells. The presence of acid phosphatase activity in the rough endoplasmic reticulum and perinuclear cisterns suggests increased production of the enzyme in the nerve cells infected with Venezuelan equine encephalomyelitis virus.

Budding

10.1128/jvi.8.2.232-241.1971

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Citations (11)

Central nervous system alterations as sequelae of Venezuelan equine encephalitis virus infection in the rat

The Journal of Pathology (1979)

J García-Tamayo Gabriel Carreño José Esparza

Alterations of the Central Nervous System (CNS) in rats surviving acute infection with a virulent strain of Venezuelan Equine Encephalitis (VEE) virus were studied by light and electron microscopy. Cavitary necrosis of the cerebral cortex, macrophage activity and degenerative axonal changes were considered to be sequelae of the lesions induced during the acute phase of the infection. Mononuclear cell infiltrates of the neuropil, 3 mth after inoculation, were related to the immune response of the host. Focal lesions and mononuclear cell activity in the brain are thought to be the equivalent of the lesions induced in the CNS of humans during VEE virus infection. The findings are discussed in the light of recent reports of cerebral dysfunction occurring as a sequel of VEE virus infection in children.

Neuropil

10.1002/path.1711280206

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Citations (22)

[Submicroscopical structure of the basal membrane and basal cells of the human normal exocervix].

PubMed (1970)

Luzardo-Baptista Mj J García-Tamayo Núñez-Montiel Jt

Basal (medicine)

Basal membrane

Basal body

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