Barbara Hargrave

Old Dominion University

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Richard Heller

University of South Florida

James C. Rose

Cincinnati Children's Hospital Medical Center

Stephen J. Beebe

Old Dominion University

Frank A. Lattanzio

Eastern Virginia Medical School

Len Murray

Paul J. Meis

Columbia University

David A. Tiangco

Old Dominion University

Anna Bulysheva

University of South Florida

Cathryn Lundberg

Old Dominion University

Christopher Osgood

Old Dominion University

Cooperative Institutions

Old Dominion University

Eastern Virginia Medical School

Dominion University College

Wake Forest University

Atrium Health Wake Forest Baptist

University of California, San Francisco

University of Ljubljana

BioElectronics (United States)

University of South Florida

The University of Texas Health Science Center at San Antonio

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Guidelines for a dental office high blood pressure screening program.

PubMed (1974)

Abbey Lm Barbara Hargrave

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Citations (1)

Nanosecond Pulse Electric Field Activated-Platelet Rich Plasma Enhances the Return of Blood Flow to Large and Ischemic Wounds in a Rabbit Model

Physiological Reports (2015)

Barbara Hargrave Francis Li

Platelet-rich plasma is a therapeutic strategy used for accelerating wound healing of a wide range of tissues through the release of platelet growth factors. Here, we describe a nonchemical, safe method for preparing platelet-rich plasma using nanosecond-pulsed electric fields (nsPEFs) and investigated the effect of this platelet-rich plasma on reperfusion of blood in large skin flap or ischemic hind limb wounds in New Zealand White rabbits. Laser Doppler images of blood flow to the dorsal surface of skin flap wounds or to ischemic hind limb wounds were obtained from wounds treated with 0.9% saline or nanosecond-pulsed electric field prepared platelet-rich plasma (nsPRP). Reperfusion in the skin flap wounds was greater in the nsPRP-treated wounds than in the wounds treated with saline on postoperative days 3 (P < 0.001) and 21 (P < 0.03). Reperfusion in the ischemic hind-limb treated with nsPRP was greater than in the saline-treated limb on post-operative Day 3 (P < 0.001), post-operative week 1 (P < 0.025) and post-operative week 4 (P < 0.015). In the hind limb ischemic tissue, the number of endothelial cells, collagen, and cells containing vascular endothelial growth factor (VEGF) was greater in the nsPRP-treated tissue. These results demonstrate that nsPRP improves blood flow in large surgical skin wounds and in ischemic wounds.

Hindlimb

10.14814/phy2.12461

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Human Platelet Gel Supernatant Inactivates Opportunistic Wound Pathogens on Skin

Europe PMC (PubMed Central) (2015)

Chelsea M. Edelblute Amy Donate Barbara Hargrave Loreé C. Heller

Activation of human platelets produces a gel-like substance referred to as platelet rich plasma or platelet gel. Platelet gel is used clinically to promote wound healing; it also exhibits antimicrobial properties that may aid in the healing of infected wounds. The purpose of this study was to quantify the efficacy of human platelet gel against the opportunistic bacterial wound pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus on skin. These opportunistic pathogens may exhibit extensive antibiotic resistance, necessitating the development of alternative treatment options. The antimicrobial efficacy of platelet gel supernatants was quantified using an in vitro broth dilution assay, an ex vivo inoculated skin assay, and in an in vivo skin decontamination assay. Human platelet gel supernatants were highly bactericidal against A. baumannii and moderately but significantly bactericidal against S. aureus in vitro and in the ex vivo skin model. P. aeruginosa was not inactivated in vitro; a low but significant inactivation level was observed ex vivo. These supernatants were quite effective at inactivating a model organism on skin in vivo. These results suggest application of platelet gel has potential clinical applicability, not only in the acceleration of wound healing, but also against relevant bacteria causing wound infections.

Ex vivo

Acinetobacter baumannii

Human skin

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Renal and Cardiovascular Effects of Atrial Natriuretic Peptide in Fetal Sheep

Pediatric Research (1989)

Barbara Hargrave Harriet S. Iwamoto Abraham M. Rudolph

Atrial natriuretic peptide

10.1203/00006450-198907000-00001

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Citations (11)

Nanosecond Pulse Electric Field Activation of Platelet-Rich Plasma Reduces Myocardial Infarct Size and Improves Left Ventricular Mechanical Function in the Rabbit Heart

Journal of ExtraCorporeal Technology (2012)

Barbara Hargrave Francis Li

In the current study, we used the novel, nonchemical method of nanosecond pulsed electric fields (nsPEF) to investigate the efficiency of a protocol involving the in vivo treatment of the ischemic and reperfused heart and heart cells in culture with platelet-rich plasma (PRP). Associated with the restoration of blood flow to the ischemic tissue is a phenomenon referred to as “ischemic reperfusion injury.” Clinically a type of reperfusion injury occurs during coronary bypass surgery once blood perfusion to the heart is restarted. Although the restoration of oxygen to ischemic myocardial cells is critical for tissue survival, reperfusion causes myocardial oxidative stress, attributable in part to the increased production of reactive oxygen species (ROS). Enhanced ROS production is associated with mitochondrial damage. Adult female New Zealand white rabbits were anesthetized and a left thoracotomy performed to expose the heart. The distal segment of the left anterior descending coronary artery was occluded for 15 minutes and then released so reperfusion of the tissue could occur. PRP (.21 mg/heart) or saline was injected into the ischemic area of the myocardium. Mechanical function of the left ventricle was analyzed using a Millar catheter attached to a Micro-Med Analysis System. H9c2 cells in culture were treated with 1 mL of nsPEF activated PRP (1.05 mg/flask) for 24 hours before analysis for ROS production or mitochondrial depolarization damage). The left ventricle contracted and relaxed faster and infarct size was reduced in hearts treated with PRP compared with saline. ROS production and mitochondrial depolarization were reduced in H9c2 cells treated with PRP and stimulated with hydrogen peroxide. These results provide evidence that nsPEFs can successfully be used to prepare PRP and that the PRP is functional in heart protection possibly by reducing ROS generation and stabilizing the mitochondria of the ischemic/reperfused heart.

Left coronary artery

10.1051/ject/201244198

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Citations (26)

351. Gene Electrotransfer of pVEGF in a Porcine Model to Induce Angiogenesis and Improve Cardiac Function

Molecular Therapy (2015)

Richard Heller Anna Bulysheva Cathryn Lundberg Niculina Burcua Len Murray

Nonviral gene therapy is an attractive method for treating cardiovascular disease; however, achieving appropriate gene expression levels is often problematic. Gene electro transfer (GET) can be safely used to enhance expression. We have previously reported on establishing GET protocols to safely and effectively delivering plasmids encoding luciferase, green fluorescent protein or VEGFA directly to the heart in a porcine model. In the current study, we tested whether gene delivery of VEGF via GET could induce angiogenesis and improve cardiac function in a porcine ischemic model. Domestic farm pigs were anesthetized and intubated followed by median sternotomy to expose the heart. The left anterior descending coronary artery was partially tied off to induce localized ischemia. Animals received no treatment, injection of pVEGF or injection of pVEGF with electro transfer. Treatment was performed at four sites surrounding the ischemic region on the border of viable and ischemic myocardium. A SPY Intraoperative Perfusion Assessment System was utilized to determine the area of ischemia and quantify the approximate reduction in perfusion and to identify the area of ischemia before treatment. The animals were followed for seven weeks. Ultrasound evaluation and arteriograms were performed prior to surgery, post treatment, 2 weeks post treatment and 7 weeks post treatment. Arteriograms suggest the growth of blood vessels as early as 2 weeks. Spy analysis revealed significant increase in perfusion within the ischemic zone in animals treated with pVEGF +GET compared to controls. Echography showed improved cardiac output was significantly greater (p<0.03) 14 days post-infarct in GET treated animals compared to values immediately after surgery. Based on these results, gene delivery to the heart can be successfully accomplished using in vivo GET and evidence suggests the potential for a therapeutic effect.

Median sternotomy

10.1016/s1525-0016(16)33960-0

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Citations (0)

Nanosecond pulse electric field (nanopulse): A novel non-ligand agonist for platelet activation

Archives of Biochemistry and Biophysics (2007)

Jue Zhang P F Blackmore Barbara Hargrave Shu Xiao Stephen J. Beebe

Nanosecond

Calcium in biology

10.1016/j.abb.2007.12.009

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Citations (127)

Cocaine Increases Intracellular Calcium and Reactive Oxygen Species, Depolarizes Mitochondria, and Activates Genes Associated With Heart Failure and Remodeling

Cardiovascular Toxicology (2005)

Frank A. Lattanzio David A. Tiangco Christopher Osgood Stephen J. Beebe Julie A. Kerry

Calcium in biology

10.1385/ct:5:4:377

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Citations (34)

3,4-Methylenedioxymethamphetamine Alters Left Ventricular Function and Activates Nuclear Factor-Kappa B (NF-kB) in a Time and Dose Dependent Manner

International Journal of Molecular Sciences (2010)

David A. Tiangco Sapna Halcomb Frank A. Lattanzio Barbara Hargrave

3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug with cardiovascular effects that have not been fully described. In the current study, we observed the effects of acute MDMA on rabbit left ventricular function. We also observed the effects of MDMA on nuclear factor-kappa B (NF-κB) activity in cultured rat ventricular myocytes (H9c2). In the rabbit, MDMA (2 mg/kg) alone caused a significant increase in heart rate and a significant decrease in the duration of the cardiac cycle. Inhibition of nitric oxide synthase (NOS) by pretreatment with L-NAME (10 mg/kg) alone caused significant dysfunction in heart rate, systolic pressure, diastolic pressure, duration of relaxation, duration of cardiac cycle, and mean left ventricular pressure. Pretreatment with L-NAME followed by treatment with MDMA caused significant dysfunction in additional parameters that were not abnormal upon exposure to either compound in isolation: duration of contraction, inotropy, and pulse pressure. Exposure to 1.0 mM MDMA for 6 h or 2.0 μM MDMA for 12 h caused increased nuclear localization of NF-κB in cultured H9c2 cells. The current results suggest that MDMA is acutely detrimental to heart function and that an intact cardiovascular NOS system is important to help mitigate early sequelae in some functional parameters. The delayed timing of NF-κB activation suggests that this factor may be relevant to MDMA induced cardiomyopathy of later onset.

MDMA

Ventricular pressure

Lusitropy

10.3390/ijms11124743

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Citations (11)

Gene Electro Transfer to Left Ventricular Myocardium in Rat and Porcine Models

IFMBE proceedings (2015)

Anna Bulysheva Barbara Hargrave Nina Burcus Cathryn Lundberg Liam Murray

Gene transfer

10.1007/978-981-287-817-5_86

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Citations (0)