Abstract Biodegradable polymeric nanoparticles have gained importance since they improve the therapeutic value of various water insoluble drugs and bioactive molecules by improving bioavailability, solubility and retention time. Nanosized drug delivery systems provide enhanced pharmacokinetic and pharmacodynamic control compared to free drugs. In this study, cationic gelatin nanoparticles were first prepared by the two step desolvation method and then their in vitro Paclitaxel (Ptx) delivery ability was evaluated. Resulting nanoparticles were analyzed for their in vitro cytotoxic effect in two different cell lines. The experimental results showed that spherical gelatin nanoparticles had a zeta potential of approximately +15.5 mV and the average size of the particles was 10.3±0.42 nm. Ptx release from gelatin nanoparticles exhibited an initial burst release followed by diffusion of the drug in a sustained manner and finally polymer erosion occured up to 38 days. In vitro cell viabilitiy analysis showed that Ptx loaded gelatin nanoparticles inhibited the growth of A549 and HT29 cells more effectively than the free drug. Based on these findings, it can be stated that cationic gelatin nanoparticles may be a promising therapeutic strategy for the treatment of many cancers.
Medicinal plants in the world are natural antioxidant sources as they contain some secondary metabolites such as phenolic acids and flavonoids. Therefore, they are used to prevent or treat many diseases in many parts of the world. However, the use of antioxidants in cancer treatment is still controversial. Accordingly, the phenolic composition and antioxidant potential of the ethanol extracts of Momordica charantia L. (Cucurbitaceae) and Datura stramonium L. (Solanaceae), which are used traditionally in Turkey and have cytotoxic potential on human cancer cells, were investigated. The antioxidant assays (DPPH, metal chelating, phosphomolybdenum, and ferric reducing power) were applied to the ethanol extracts of the fruits of M. charantia and the leaves of D. stramonium. The total phenolic and flavonoid contents were determined. The phenolic compounds contained in ethanolic extracts were identified using HPLC method. Fifteen phenolic compounds were identified in the extracts. Caffeic acid was the major constituent in both extracts. The amount of caffeic acid was detected to be 6282.51 and 15183.36 µg g-1 extract in M. charantia extract and the D. stramonium extracts, respectively. D. stramonium leaf extract showed higher antioxidant activity than M. charantia fruit extract and this activity may be associated with high phenolic compound concentrations. Hence, further studies about screening of bioactive compounds from various part of these plants will be a great importance for obtaining of source of novel natural antioxidants.
Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This study was the first to determine the in vitro biological effects of ethanolic extract from the flowering parts of C. solstitialis L. subsp. solstitialis collected from Muğla Province.The cytotoxic effect was evaluated against Daudi, A549, and HeLa cancer cells and one normal BEAS-2B cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- dipenyltetrazolium bromide) assay. Flow cytometric analysis and the caspase-3 activity assay were performed to detect apoptotic cell death. Angiogenic factor [vascular endothelial growth factor (VEGF)] secretion and the release of interleukin (IL)-1α, IL-6, and tumor necrosis factor (TNF)-α by cells treated with the extract were measured using enzyme-linked immunosorbent assay.The extract exhibited cytotoxic effects against all the cancer cell lines used but HeLa and Daudi were the most sensitive cells, with IC50 values of 63.18 μg/mL and 69.27 μg/mL, respectively. Selective cytotoxicity was observed between the HeLa and normal BEAS-2B cell lines. The extract arrested the cell cycle at the S and G2 phases. In addition, apoptotic cell death was detected in HeLa and A549 cells. Moreover, the plant extract caused a significant decrease in VEGF secretion in A549 cells and a fluctuation in IL-1α, IL-6, and TNF-α secretion in A549 and Daudi cells.These observations suggest that the flowering parts of C. solstitialis may be a potential source in the development of natural drugs for the treatment of cancer and modulation of cytokine secretion.
Analysis Of The Ecological Characteristics And Economic Impacts Of Identified Terrestrial And Aquatic Invasive Species In Turkey Seher Öztürk Murat Turan Günay Mammadova Impact Of Ecological Problems On Insect Populations Zamina Bunyatzade The Multifarious Ecosystem Services Of Asian Palmyra Palm Afra Nawar Rahman Sunehra Sayanhika Prabhashani Madhumitha Parameshwaram Lala Huseynova Aishwarya Prakash Somashree Chakma Naliprue Marma Paulraj Mosae Selvakumar Preliminary Experience Of Using Arbutus Unedo L. Specie In Apsheron Conditions (Azerbaıjan) Aynur Huseynova The Effect Of Ecology On The Human Organism Imran Akhundov Zulfiyya Iskenderova Green Bangle Movement: A Movement Of Climate Resilience, Women Empowerment, And Coastal Ecosystem Of Bangladesh And Asıa Iffat Mahjabin Nuzaba Tasannum Prerna Thapa Magar Elizabeth Ximenes Dhushanthi Balakrishnan Paulraj Selvakumar Mosae Problems Of Conservation Of Flora And Fauna Tamilla Kerimova Causes, Consequences And Prediction Of Global Climate Change As A Source Of Natural Hazards Munavvar Safarova Banu Amanullayeva The Effect Of Climate And Disease Factors On Juglans Regıa L Ramazan Mammadov Minara Hasanova Aysel Sadigli Sona Gulizada Translanguaging In Bilingual And Multilingual Classes Usıng Icts To Address Clımate Change Context Gurbanova Sevil Khalilov Rashad Asima Gureshi Sevda Asadova Khalilov Abdulhamid Development Of Fruıt Growing In Azerbaıjan. Impact Of Ecology On Fruit Growıng Munavvar Safarova Construction Of Deterministic Models Of Nonstationary Phenomena In Porous Medıa Gunel Mamadova Lala Huseynova Seylan Rahimova The Impact Of The Ecological Change On The Food Safety Hasan Mammadov Saida Bayramova Afruz Nasirova Different Biological Properties Of Algae Beria Ozçakır Birsen Atlı Dostu Gafarova, Burak Isık Mehlika Alper Ramazan Mammadov Ornamental Peppers Importance In Genetic And Ecological Sustainability Muhemet Zeki Karipçin Arzu Çığ Sevinc Guliyeva Changes In Soıl Indicators Along The Highway Due To Anthropogenıc Effect Sevinj Maharramova National Characteristics Of Environmental Security Of Azerbaıjan Majidova Laman Gafarova Dostu Environmental Effects That, Negatively Affecting Alcohol Beverage Production In Our Country And Their Removal Ways Tarana Gilicova
Microbial exopolysaccharides (EPSs) are biopolymers in the form of carbohydrates produced by many microorganisms and secreted into the external environment. EPS protects the microorganism from drying, phagocytosis, and phage effects, and acts as a barrier in stress environments such as heat, light and sound. EPSs produced for industrial purposes are generally used in areas such as food, cosmetics, petroleum and chemistry. This study was aimed to investigate in addition to basic physical and chemical properties of R. pyridinovorans EPS, in vitro its biological activities such as antioxidant properties and antiproliferative activity. The antioxidant properties of EPS were determined by DPPH and hydroxyl radical elimination. The antiproliferative activity of EPS on HT-29 and MCF-7 cell lines was determined by MTT assay. The results of study indicate that EPS from R. pyridinovorans have important biological activities. Further studies on structural and mechanism elucidation of the bacterial EPSs are still needed being carried out.
Plants continue to be a good source for developing effective anticancer agents. In this study, in vitro various biological effects of crude ethanolic extract from flowering parts of Urospermum picroides collected from the Muğla province of Turkey were investigated for the first time. Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay. The effect of the extract on cell cycle progression was detected by flow cytometric analysis. The level of VEGF, IL-1α, IL-6 and TNF-α secretion in the cells treated with the extract were measured using ELISA The extract caused a higher cytotoxic effect on Daudi cells with an IC50 value of 85.64 µg/mL than the other cells tested. The IC50 values in HeLa and A549 cells were determined to be 135.35 and 234.8 µg/ mL, respectively. The selective cytotoxicity was considered between Daudi and BEAS-2B (109.80 µg/mL) cell lines. In addition, the effect of the extract on cell cycle progression changes according to cell line used. Moreover, the extract decreased the level of secreted VEGF in treated A549 cells by 31%. In addition, the extract resulted in a significant decrease in the secretion of IL-1α, IL-6 and TNF-α cytokines in A549 and Daudi cells compared to the untreated cells. These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.
Regenerative endodontics is a developing field involving the restoration of tooth structure and re-vitality of necrotic pulp. One of the most critical clinical considerations for regenerative endodontic procedures is the disinfection of the root canal system, since infection interferes with regeneration, repair, and stem cell activity. In this study, we aimed to provide the synthesis of injectable biopolymeric tissue scaffolds that can be used in routine clinical and regenerative endodontic treatment procedures using Gelatin methacryloyl (GelMA), and to test the antimicrobial efficacy of Gelatin methacryloyl/Silver nanoparticles (GelMA/AgNP), Gelatin methacryloyl/Hyaluronic acid (GelMA/HYA), and Gelatin methacryloyl/hydroxyapatite (GelMA/HA) composite hydrogels against microorganisms that are often encountered in stubborn infections in endodontic microbiology. Injectable biocomposite hydrogels exhibiting effective antimicrobial activity and non-cytotoxic behavior were successfully synthesized. This is also promising for clinical applications of regenerative endodontic procedures with hydrogels, which are proposed based on the collected data. The GelMA hydrogel loaded with hyaluronic acid showed the highest efficacy against
