A Suganuma

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Ultrastructural Localization of Wheat Germ Agglutinin-Binding Sites in <italic>Escherichia coli</italic> with Wheat Germ Agglutinin-Gold Complexes

Journal of Electron Microscopy (1987)

Hiroyuki Morioka M. Tachibana A Suganuma

Post-embedding staining of intracellular carbohydrates on thin sections of Escherichia coli embedded in epoxy or Lowicryl K4M resin was studied by the lectin-gold technique. The labelings of WGA-gold particles were denser in the Lowicryl K4M-embedded preparation than in the Epon-embedded preparation. Gold particles which represent the localization of N-acetyl-glucosamine and N-acetyl-neuraminic acid were present on the cell wall and the cytoplasmic membrane of E. coli. The use of gold-labeling was a useful means for localizing the lectin-binding sites in bacteria.

10.1093/oxfordjournals.jmicro.a050602

Cite

Citations (3)

Cytological changes of Escherichia coli caused by polymyxin E.

PubMed (1968)

A Suganuma Kenji Hara Takahiro Kishida Kohei Nakajima J Kawamata

Polymyxin

Polymyxin B

Source

Cite

Citations (7)

Morphological Changes in Membrane Systems of <italic>Staphylococci</italic> after Different Fixation Procedures

Journal of Electron Microscopy (1979)

A Suganuma Hiroyuki Morioka

Journal Article Morphological Changes in Membrane Systems of Staphylococci after Different Fixation Procedures Get access Atsushi SUGANUMA, Atsushi SUGANUMA Search for other works by this author on: Oxford Academic PubMed Google Scholar Hiroyuki MORIOKA Hiroyuki MORIOKA *Electron Microscope Laboratory, Kyoto Prefect ural University of MedicineKyoto, 602 Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Electron Microscopy, Volume 28, Issue 1, 1979, Pages 29–35, https://doi.org/10.1093/oxfordjournals.jmicro.a050145 Published: 01 January 1979 Article history Received: 04 October 1978 Accepted: 05 December 1978 Published: 01 January 1979

10.1093/oxfordjournals.jmicro.a050145

Cite

Citations (5)

Activity of variotin, a new antifungal antibiotic.

PubMed (1959)

Shuichi Suzuki A Suganuma Tsunataro Kíshida A Mizunoya Fumio Ueda

Source

Cite

Citations (4)

Ultrastructure of <italic>Staphylococcus aureus</italic> as Revealed by Microwave Fixation

Journal of Electron Microscopy (1992)

Hiroyuki Morioka A Suganuma M. Tachibana

The ultrastructure of the cell wall of Staphylococcus aureus was examined at electron microscopic level using new chemical fixation techniques during microwave irradiation and the results obtained were compared with those obtained by other conventional techniques. By using microwave fixation the concentric circular or zipper-like structure was observed in the cell wall. This structure was observed also with the spray-freeze-etch technique but not in thin section of the cells chemically fixed by conventional technique. For chemical fixative, glutaraldehyde is more advantageous than OsO4 as a concomitant fixative during microwave irradiation and postfixation by OsO4 is unnecessary and rather harmful for the preservation of the ultrastructure. The function of the observed structure is briefly discussed.

Fixative

Glutaraldehyde

10.1093/oxfordjournals.jmicro.a050924

Cite

Citations (2)

Ultrastructural localization of carbohydrates on thin sections of Staphylococcus aureus with silver methenamine and wheat germ agglutinin-gold complex

Journal of Bacteriology (1987)

Hiroyuki Morioka M. Tachibana A Suganuma

Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. Labeling by wheat germ agglutinin-gold particles was observed on both the cell wall and the cross wall, and the staining pattern resembled that of silver methenamine staining. Therefore, the labeling was considered to be due to N-acetylglucosamine of teichoic acid. The combination of two types of cytochemical techniques was useful to localize and characterize the carbohydrates of the bacterial cell.

Teichoic acid

Germ tube

Silver stain

Periodic acid

10.1128/jb.169.3.1358-1362.1987

Cite

Citations (20)

Localization of sugar-binding sites in Staphylococcus aureus using gold-labeled neoglycoprotein.

Journal of Histochemistry & Cytochemistry (1994)

Hiroyuki Morioka A Suganuma M. Tachibana

We studied post- and pre-embedding staining of sugar-binding sites on thin sections of Staphylococcus aureus with an electron microscopic neoglycoprotein-gold technique. Although gold particles of cellobiosyl bovine serum albumin (BSA)-glycosylated BSA-, lactosyl BSA-, and melibiosyl BSA-gold did not label, heavy labeling of N-acetylglucosaminide-BSA-gold was observed in both the cell wall and the cytoplasm on Spurr-embedded thin sections of S. aureus. Inhibition of labeling with wheat germ agglutinin-biotin and N-acetylglucosaminidase indicated that the labeling was due to N-acetylglucosamine. These data suggested that molecules that bind specifically with N-acetylglucosamine occur in the cell wall and cytoplasm of S. aureus. Pre-embedding staining revealed that these molecules are abundant at the surface of the cell wall and that the abundance differs depending on the bacterial strain. An N-acetylglucosamine-specific lectin-like substance, glucosaminidase, and toxins are proposed as candidates for molecules responsible for the labeling, and the possible functional significance of the findings is discussed briefly.

Bovine serum albumin

Negative stain

Acetylglucosamine

10.1177/42.12.7983361

Cite

Citations (4)