Cell scaffold material is the framework of regeneration in tissue engineering.The property of cell scaffold material is the direct effect on the adhesion,proliferation and metabolism functions.Therefore,cell scaffold material is one of the critical factors in tissue engineering.Currently,the scaffold material of tissue engineering is categorized as the synthesized absorbable macromolecule polymer,natural material extracts and extracellular matrix.Based on the pertinent iterature in recent years,the research progress of scaffold material in tissue engineering was summarized in this paper.
AIM To investigate how Staphylococcus aureus influences gene expressions of cell adhesion molecules(E-Selectin,PECAM-1,VCAM-1) and MCSF-1 in left ventricular myocardium and explore the cellular immunology mechanism of infective endocarditis.METHODS Seven rabbits received 5×10~(6) colony forming units Staphylococcus aureus i.v.,9 rabbits underwent mitral surgery and 8 rabbits underwent both interventions in combination.The hearts were explanted 24 h after the intervention and observed by naked eye and electron microscope.mRNA abundance was determined by semiquantitative duplex RT-PCR.Results were compared with those in control group consisting of 7 untreated rabbits.(RESULTS) Whereas the bacteraemia did not lead to upregulated mRNA levels of any studied molecules,mitral valve surgery caused significantly higher relative expression levels of E-Selectin,PECAM-1 and MCSF1,but not of VCAM-1.The combination of bacteremia and mitral valve surgery caused an uneven expression pattern with a significant upregulation of E-Selectin and MCSF-1 and a downregulation of PECAM-1 and VCAM-1.CONCLUSION Staphylococcus aureus leads to changes of regulations of specific cell adhesion molecules and MCSF-1 in left ventricular myocardium after surgical procedure,thus resulting in the development of infective endocarditis.
Objective To study the early adhesive effect of bacteria on intracardiac transplanted materials in vivo. Methods Fifty-three rabbits were transplanted with different material intracardiac patches,eg. dacron(DAC,n = 16),glutaraldehyde fixed autograft pericardium(GAP, n = 18),cryopreserved allograft(AG,n = 19). The patches were transplanted on the mitral valve establishing mitral insufficiency. Staphylococcus aureus of 5 × 106 colony forming units CFU were injected 6 hours postoperatively into auricular vein. The rabbits were sacrificed 48 h after injection then the patches were taken out from heart,cullivated and analysed. Results Ten rabbits(2 DAC,4 GAP and 4 AG) dead early and excluded from the study.Bacteria adhesive patches were found in 8/14 DAC,5/14 GAP and 6/15 AG groups. Differences of the CFU number in bacteria positive pathches were not significant among the different group(P 0.05). Conclusion The study demonstrates that there is no significant difference in the early effect of bacteria adhesion between intracardiac biological implants and artificial material prosthesis.
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