Keith Backman

University of Saskatchewan

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Mark Ptashne

Memorial Sloan Kettering Cancer Center

R. Balakrishnan

Sree Balaji Dental College and Hospital

A Maruya

University of Freiburg

Mary Jane O'Connor

BioTechnica (United States)

Boris Magasanik

Massachusetts Institute of Technology

Randolph T. Hatch

BioTechnica (United States)

Robert T. Sauer

Massachusetts Institute of Technology

George H. Shimer

NKT Therapeutics (United States)

Barbara J Meyer

University of California, Berkeley

Francisco Bolívar

Universidad Nacional Autónoma de México

Cooperative Institutions

Harvard University

BioTechnica (United States)

Massachusetts Institute of Technology

Children's Hospital of Eastern Ontario

Rutgers, The State University of New Jersey

University of Ottawa

University of California, San Francisco

Howard Hughes Medical Institute

Triangle

Becton Dickinson (United States)

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Patents and literature

Applied Biochemistry and Biotechnology (1990)

Jonathan S. Dordick Keith Backman R. Balakrishnan Roger Brent Mark Ptashne

Protein Engineering

Protein design

Biocatalysis

Directed Molecular Evolution

Site-directed mutagenesis

Folding (DSP implementation)

Molecular engineering

Denaturation (fissile materials)

Metabolic Engineering

10.1007/bf02798396

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A general method for the purification of restriction enzymes

Nucleic Acids Research (1978)

Patricia J. Greene Herbert L. Heyneker Francisco Bolívar Raymond L. Rodriguez Mary C. Betlach

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

Hydroxylapatite

Cloning (programming)

Restriction map

Restriction fragment

10.1093/nar/5.7.2373

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Citations (314)

Genetic and Physical Studies on the Replication of ColE1-type Plasmids

Cold Spring Harbor Symposia on Quantitative Biology (1979)

Keith Backman Mary C. Betlach H W Boyer Stephen Yanofsky

The mechanisms of replication of extrachromosomal elements such as plasmid ColE1 are determined by both host-specific and replicon-specific components. Replication of ColE1 differs from that of many other prokaryotic replicons in several ways: it is unidirectional (Inselburg 1974; Lovett et al. 1974), requires the polymerizing activity of DNA polymerase I (Kingsbury and Helinski 1973), proceeds in the absence of protein synthesis (Clewell 1972), and maintains a high number of genomes per cell (Clewell and Helinski 1972). There is evidence that no ColE1-encoded polypeptide is required for its replication (Donoghue and Sharp 1978; Kahn and Helinski 1978). We are investigating the replication of plasmids derived from pMB1, a plasmid structurally and functionally very similar to ColE1 (Betlach et al. 1976). We have identified the plasmid-specified genetic information of pMB1 which is required for its own replication, and we have constructed in vitro site-specific alterations of that genetic information. Examination of the...

ColE1

Extrachromosomal DNA

Replicon

Replication

Pre-replication complex

Autonomously replicating sequence

Minichromosome maintenance

T-DNA Binary system

10.1101/sqb.1979.043.01.012

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Citations (86)

TRANSCRIPTIONAL AND TRANSLATIONAL (?) CONTROL OF THE λ REPRESSOR GENE (cI)

Elsevier eBooks (1976)

Zafri Humayun Barbara J Meyer Robert T. Sauer Keith Backman Mark Ptashne

Ribosomal binding site

Translational regulation

10.1016/b978-0-12-518550-9.50014-7

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Regulation at the glnL -Operator-Promoter of the Complex glnALG Operon of Escherichia coli

Journal of Bacteriology (1983)

Shizue Ueno-Nishio Keith Backman Boris Magasanik

We identified the portion of the glnALG operon that contains the promoter and operator of the glnLG suboperon and showed that the product of glnG represses transcription initiated at glnLp .

gal operon

Operator (biology)

Transcription

trp operon

L-arabinose operon

10.1128/jb.153.3.1247-1251.1983

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Citations (43)

A cautionary note on the use of certain restriction endonucleases with methylated substrates

Gene (1980)

Keith Backman

Recognition sequence

Cytosine

Cleavage (geology)

Isoschizomer

Sequence (biology)

10.1016/0378-1119(80)90097-9

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Citations (74)

Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli

Journal of Bacteriology (1982)

Yuezhou Chen Keith Backman Boris Magasanik

DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was cloned on plasmid pBR322. By using recombination in vitro, we mapped the GlnC mutation to a region between glnA and glnG. This region defines a gene, glnL, which codes for a trans-acting product; the GlnC mutant produces an altered product. The glnL product plays a key role in the communication of information concerning the quality and abundance of the nitrogen source in the growth medium to a destination responsible for the regulation of glnA and other genes for enzymes responsible for nitrogen utilization.

Structural gene

10.1128/jb.150.1.214-220.1982

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Citations (122)

Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.

Proceedings of the National Academy of Sciences (1981)

Keith Backman Yuezhou Chen Boris Magasanik

We have cloned and characterized a fragment of the Escherichia coli chromosome spanning glnA, the structural gene for glutamine synthetase (L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. The fragment also carriers glnG, whose product is necessary for regulation of glnA expression, and a previously unidentified gene whose function we have not discovered. Transcription of glnA and the newly identified gene occurs divergently from a region between the two genes. Transcription of glnA proceeds toward glnG, which is transcribed in the same direction. A region of DNA between glnA and glnG contains genetic information whose loss may result in the inability to reduce expression of glnA and other operons in response to ammonia (the GlnC phenotype).

Transcription

Structural gene

10.1073/pnas.78.6.3743

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Citations (232)

In vivoevents in the generation of_pBR322

Nucleic Acids Research (1986)

Keith Backman

Journal Article In vivo events in the generation of p BR322 Get access Keith Backman Keith Backman BioTechnica InternationalInc., 85 Bolton St., Cambridge, MA 02140, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 14, Issue 3, 11 February 1986, Page 1542, https://doi.org/10.1093/nar/14.3.1542 Published: 11 February 1986 Article history Received: 02 December 1985 Published: 11 February 1986

10.1093/nar/14.3.1542

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Citations (2)

Ligase Chain Reaction

Humana Press eBooks (2003)

George H. Shimer Keith Backman

The ability to detect low numbers of DNA target sequences has been greatly enhanced by the development of geometric amplification techniques, most of which employ enzymatic methods to replicate a target DNA or RNA sequence in an autocatalytic fashion. These techniques have become so widely used that a new journal, PCR Methods and Applications, has arisen in an attempt to consolidate literature relevant to polymerase chain reactions (PCR) (1) and other amplification technologies.

Applications of PCR

Autocatalysis

Chain reaction

10.1385/0-89603-297-3:269

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Citations (1)