Fatima Barragan

University of California, San Francisco

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Linda C. Giudice

University of California, San Francisco

Juan C. Irwin

University of California, San Francisco

David W. Erikson

Oregon National Primate Research Center

Joseph C. Chen

BioMarin (United States)

Terhi Piltonen

Oulu University Hospital

Shaina Balayan

University of California, San Francisco

Kim Chi Vo

Human Longevity (United States)

Lusine Aghajanova

Stanford Medicine

Trimble Spitzer

Uniformed Services University of the Health Sciences

Gabriel Goldfien

University of California, San Francisco

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University of California, San Francisco

Reproductive Science Center

Stanford University

University of California System

Bellvitge University Hospital

Stanford Medicine

City College of San Francisco

Gladstone Institutes

Baylor College of Medicine

University of California, Davis

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[Salivary testosterone and its applications].

PubMed (1986)

Navarro Ma Fatima Barragan C Villabona Andreu Pujol Bonnín Mr

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Citations (2)

Mesenchymal Stem/Progenitors and Other Endometrial Cell Types From Women With Polycystic Ovary Syndrome (PCOS) Display Inflammatory and Oncogenic Potential

The Journal of Clinical Endocrinology & Metabolism (2013)

Terhi Piltonen J. Chen David W. Erikson Trimble Spitzer Fatima Barragan

Endometrium in polycystic ovary syndrome (PCOS) presents altered gene expression indicating progesterone resistance and predisposing to reduced endometrial receptivity and endometrial cancer.We hypothesized that an altered endocrine/metabolic environment in PCOS may result in an endometrial "disease phenotype" affecting the gene expression of different endometrial cell populations, including stem cells and their differentiated progeny.This was a prospective study conducted at an academic medical center.Proliferative-phase endometrium was obtained from 6 overweight/obese PCOS (National Institutes of Health criteria) and 6 overweight/obese controls. Microarray analysis was performed on fluorescence-activated cell sorting-isolated endometrial epithelial cells (eEPs), endothelial cells, stromal fibroblasts (eSFs), and mesenchymal stem cells (eMSCs). Gene expression data were validated using microfluidic quantitative RT-PCR and immunohistochemistry.The comparison between eEP(PCOS) and eEP(Ctrl) showed dysregulation of inflammatory genes and genes with oncogenic potential (CCL2, IL-6, ORM1, TNAIFP6, SFRP4, SPARC). eSF(PCOS) and eSF(Ctrl) showed up-regulation of inflammatory genes (C4A/B, CCL2, ICAM1, TNFAIP3). Similarly, in eMSC(PCOS) vs eMSC(Ctrl), the most up-regulated genes were related to inflammation and cancer (IL-8, ICAM1, SPRR3, LCN2). Immunohistochemistry scoring showed increased expression of CCL2 in eEP(PCOS) and eSF(PCOS) compared with eEP(Ctrl) and eSF(Ctrl) and IL-6 in eEP(PCOS) compared with eEP(Ctrl).Isolated endometrial cell populations in women with PCOS showed altered gene expression revealing inflammation and prooncogenic changes, independent of body mass index, especially in eEP(PCOS) and eMSC(PCOS), compared with controls. The study reveals an endometrial disease phenotype in women with PCOS with potential negative effects on endometrial function and long-term health.

10.1210/jc.2013-1923

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Citations (94)

Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production

Fertility and Sterility (2013)

Joseph C. Chen David W. Erikson Terhi Piltonen M. Renée Umstattd Meyer Fatima Barragan

10.1016/j.fertnstert.2013.06.007

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Citations (47)

Human endometrial co-culture system reveals novel cell-specific effects of nonoxynol-9 on endometrial transcriptome and chemokine secretion

Fertility and Sterility (2012)

J.C. Chen David W. Erikson Fatima Barragan Juan C. Irwin Warner C. Greene

10.1016/j.fertnstert.2012.07.099

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Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts

Human Reproduction (2014)

Joseph C. Chen Brittni Johnson David W. Erikson Terhi Piltonen Fatima Barragan

STUDY QUESTIONHow does seminal plasma (SP) affect the transcriptome of human primary endometrial epithelial cells (eEC) and stromal fibroblasts (eSF)?

10.1093/humrep/deu047

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Citations (64)

Human Endometrial Fibroblasts Derived from Mesenchymal Progenitors Inherit Progesterone Resistance and Acquire an Inflammatory Phenotype in the Endometrial Niche in Endometriosis1

Biology of Reproduction (2016)

Fatima Barragan Juan C. Irwin Shaina Balayan David W. Erikson Joseph C. Chen

Human endometrium undergoes cyclic regeneration involving stem/progenitor cells, but the role of resident endometrial mesenchymal stem cells (eMSC) as progenitors of endometrial stromal fibroblasts (eSF) has not been definitively demonstrated. In endometriosis, eSF display progesterone (P4) resistance with impaired decidualization in vivo and in vitro. To investigate eMSC as precursors of eSF and whether endometriosis P4 resistance is inherited from eMSC, we analyzed transcriptomes of eutopic endometrium eMSC and eSF isolated by fluorescence-activated cell sorting (FACS) from endometriosis (eMSCendo, eSFendo) and controls (eMSCcontrol, eSFcontrol) and their derived primary cultures. Differentially expressed lineage-associated genes (LG) of FACS-isolated eMSC and eSF were largely conserved in endometriosis. In culture, eSFcontrol maintained in vitro expression of a subset of eSF LG and decidualized in vitro with P4 The eMSCcontrol cultures differentiated in vitro to eSF lineage, down-regulating eMSC LG and up-regulating eSF LG, showing minimal transcriptome differences versus eSFcontrol cultures and decidualizing in vitro. Cultured eSFendo displayed less in vitro LG stability and did not decidualize in vitro. In vitro, eMSCendo differentiated to eSF lineage but showed more differentially expressed genes versus eSFendo cultures, and did not decidualize in vitro, demonstrating P4 resistance inherited from eMSCendo Compared to controls, cultures from tissue-derived eSFendo uniquely had a pro-inflammatory phenotype not present in eMSCendo differentiated to eSF in vitro, suggesting divergent niche effects for in vivo versus in vitro lineage differentiation. These findings substantiate eMSC as progenitors of eSF and reveal eSF in endometriosis as having P4 resistance inherited from eMSC and a pro-inflammatory phenotype acquired within the endometrial niche.

Progenitor

10.1095/biolreprod.115.136010

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Citations (137)

Global Transcriptome Abnormalities of the Eutopic Endometrium From Women With Adenomyosis

Reproductive Sciences (2016)

Christopher N. Herndon Lusine Aghajanova Shaina Balayan David W. Erikson Fatima Barragan

Adenomyosis

Myometrium

10.1177/1933719116650758

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Citations (88)

Pulmonary asymmetry in pediatrics: which is the good side?

M. Pitarch Diago Castañeda Martínez V. P. Beltrán Salazar Carmina Durán Feliubadaló Duran Martinez

Poster: ECR 2018 / C-3056 / Pulmonary asymmetry in pediatrics: which is the good side? by: M. PITARCH DIAGO 1, C. Martin Martinez1, V. P. Beltran Salazar1, C. Duran Feliubadalo1, D. Rodriguez Martinez1, B. Del Rio2, F. Bosch Barragan1, A. Gonzalez Lopez1, A. B. A. B. CASTRO GARCIA2; 1Sabadell/ES, 2Terrassa/ES

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Stromal fibroblasts from perimenopausal endometrium exhibit a different transcriptome than those from the premenopausal endometrium†

Biology of Reproduction (2017)

David W. Erikson Fatima Barragan Terhi Piltonen Joseph C. Chen Shaina Balayan

Human endometrium undergoes extensive regeneration on a cyclic basis in premenopausal women and likely occurs through the contribution of stem/progenitor cells. Menopause results in the permanent cessation of menstrual cycles and is preceded by perimenopause, a period of several years in which endocrine and biological changes occur and is a period of risk for endometrial proliferative disorders. The objectives of this study were to identify endometrial mesenchymal stem cells (eMSC) and endometrial stromal fibroblasts (eSF) in endometrium of perimenopausal women and perform expression profile analysis of perimenopausal eMSC and eSF to gain insight into the biology of stem/progenitor and lineage cell populations during the transition to menopause. Endometrial tissue was collected from perimenopausal and premenopausal women (n = 9 each). Microarray analysis was performed on fluorescence-activated cell sorting-isolated eSF and eMSC, and data were validated by quantitative real-time PCR. Principal component analysis showed that cells clustered into three distinct groups in 3-dimensional space: perimenopausal eMSC and premenopausal eMSC clustered together, while perimenopausal eSF and premenopausal eSF formed two discrete clusters separate from eMSC. Hierarchical clustering revealed a branching pattern consistent with principle clustering analysis results, indicating that eMSC from premenopausal and perimenopausal women exhibit similar transcriptomic signatures. Pathway analysis revealed dysregulation of cytoskeleton, proliferation, and survival pathways in perimenopausal vs. premenopausal eSF. These data demonstrate that cell populations have altered gene expression in perimenopausal vs. premenopausal endometrium, and that perimenopausal eSF had altered pathway activation when compared to premenopausal eSF. This study provides insight into aging endometrium with relevance to function in reproductively older women.

10.1093/biolre/iox092

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Citations (16)

Pro-inflammatory effects of seminal plasma on human endometrium: implications for implantation and human immunodeficiency virus (HIV) transmission

Fertility and Sterility (2012)

Brett A. Johnson J. Chen Nadia R. Roan Fatima Barragan Juan C. Irwin

Viability assay

Interleukin 8

10.1016/j.fertnstert.2012.07.801

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