Kiseleva Tv

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Liapkov Bg

Khotimchenko Sa

Federal Research Centre of Nutrition and Biotechnology

Martynova Ea

Federal Research Centre of Nutrition and Biotechnology

Kokhanova IuA

Abbakumov As

Samsonova ZhV

Samsonov Ma

Voinov Di

Institute of Cytology and Genetics

Orlova Ng

АЕ

А.М. Егоров

Lomonosov Moscow State University

Cooperative Institutions

Federal Research Centre of Nutrition and Biotechnology

Lomonosov Moscow State University

Russian Academy of Sciences

Moscow State University

Institute of Cytology and Genetics

Chumakov Institute of Poliomyelitis and Viral Encephalitides

Volga State University of Technology

O.Ya. Usikov Institute for Radiophysics and Electronics

Semenov Institute of Chemical Physics

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

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[Plasma triacylglycerols in alimentary obesity].

PubMed (1991)

Liapkov Bg Martynova Ea Voinov Di Beiul Ea Kiseleva Tv

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[Health education in preventing diseases of the teeth and periodontium].

PubMed (1985)

Kiseleva Tv

Periodontium

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[Phycotoxins of ASP, PSP and DSP groups in aquaris organisms].

Kokhanova IuA Kiseleva Tv Khotimchenko Sa

In this article the results of analysis of ASP, PSP and DSP phycotoxins content in aquatic organisms are presented. Methods of determination of toxins were ELISA and HPLS.

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[Cholesterol metabolism in patients with ischemic heart disease with varying supplies of omega-3 polyunsaturated fatty acids].

PubMed (1994)

Samsonov Ma Liapkov Bg Abbakumov As Kiseleva Tv Pogozheva Av

Patients with ischemic heart disease and with hyperlipidemia of the IIa, IIb and IV types as well as not exhibiting any pronounced alterations in cholesterol metabolism were treated with various amounts of omega-3 polyunsaturated fatty acids (PUFA) introduced into the basic antiatherosclerotic diet: 1 group--content of the essential factor 0.2 g/day, 2 group--increased content of natural products with PUFA up to 1 g/day, 3 group--increased PUFA content due to the drug "euconol" 5 g/day. Administration of the PUFA omega-3 into the basic antiatherosclerotic diet contributed to development of adequate clinico-biochemical alterations; under these conditions content of alpha-cholesterol in blood correlated directly to concentration of polyunsaturated cholesterol esters and PUFA omega-3 (eicosapentaenate and docosahexaenate) as well as reverse correlation was found between content of total cholesterol and these molecular forms of cholesterol esters studied. Enrichment of the diet with PUFA omega-3 did not cause any alterations in content of primary bile acids in blood thus suggesting that PUFA omega-3 did not affect the cholesterol catabolism. These data enabled us to plot the graph for individual evaluation of cholesterol metabolism in patients with ischemic heart disease involving alterations in the spectrum of esterified cholesterols in blood before and after the treatment course.

Hyperlipidemia

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[Characteristics of lipids of microbial origin with regard to triglyceride composition and comparison of them to plant oils].

PubMed (1993)

Liapkov Bg Kiseleva Tv

The triacylglycerol composition of lipids isolated from the yeasts Candida guilliermondii and C. humicola and of food vegetable oils was investigated by HPLC. The triacylglycerol composition of samples was compared using the program "Nearest neighbour" and by plotting images with the help of the software package STATGRAPHICS. The methods used allow us to conclude that microbial lipids are rather promising.

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[Molecular types of breast milk triacylglycerols at various stages of lactation].

PubMed (1992)

Liapkov Bg Kiseleva Tv

The composition of individual triacylglycerols was studied in women milk in various periods of lactation. The spectra of triacylglycerols were studied in the early lactation milk, in the mature milk and in the late lactation milk using a method of "main components", the program of "immediate neighbour" as well as by construction of graphic plot (by means of a set "Statgraphics"). The methods of calculation used enabled one to find great differences in the triacylglycerol spectrum of mature milk as compared with those of milk in the early and late periods of lactation.

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[Triacylglycerols in human milk and milk mixtures for infant feeding].

PubMed (1992)

Liapkov Bg Kiseleva Tv

High-performance liquid chromatography and computer program for identification of individual triacylglycerols (TAG) were employed for investigation of TAG composition of mature breast milk and 8 specimens of dry milk food for infants. The comparison was made by 15 molecular types of breast milk TAG using methods "close neighbour" and "star symbol plot" (Statgraphics). The composition appeared quite different. The design of artificial nutrition for infants basing on identity of fatty acids is believed inadequate as it cannot reproduce Tag composition of natural breast milk.

Infant nutrition

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[Optimization of a method of solid-phase immunoenzyme analysis for determination of chloramphenicol in milk].

PubMed (1999)

Kolosova AIu Samsonova ZhV А.М. Егоров Shevaleva Sa Orlova Ng

The method of solid-phase enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in milk was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied. Conditions providing CAP monitoring in human blood serum in the clinical range were optimized. Matrix effect on the assay results was studied. The specificity of the analytical system was investigated and the reagents stability was examined. The method developed permits CAP concentration to be determined in human blood serum, diluted 1/100, in the linear range from 0.1 to 100 ng/ml. The assay is characterized by high sensitivity (0.08 ng/ml) and good reproducibility (CV < 10.8%), assay time is about 3 hours. The correlation coefficient with HPLC is 0.977.

Conjugate

Matrix (chemical analysis)

Solid phase extraction

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Citations (1)