Psoralen is an effective active component extracted from Psoraleacorylifolia, which can promote bone formation in osteoporotic animals. However, to the best of our knowledge, its effect on fracture healing has not yet been examined. In the present study, open femur fractures were created in ovariectomy (OVX)‑induced osteoporotic mice. OVX mice were treated with psoralen (psoralen+OVX group) or physiological saline (OVX group) by oral gavage. Radiographic and histological results demonstrated progressed callus consolidation in the psoralen+OVX group compared with the OVX group after 10 and 21 days of treatment. Qualitative histological analysis showed that the number of osteoclasts was significantly reduced in the psoralen+OVX group after treatment. Moreover, reverse transcription‑quantitative PCR analysis of callus samples showed increased expression of bone morphogenetic protein‑2 (BMP‑2) and osteoprotegerin (OPG), and decreased expression of receptor activator of nuclear factor‑κB ligand (RANKL) at 10 and 21 days post injury in the psoralen+OVX group compared with the OVX group. Furthermore, western blot analysis showed that psoralen significantly increased the expression of estrogen receptor (ER)‑α, but had no effect on ER‑β expression; these results were further confirmed by immunohistochemistry. To conclude, these results indicated that psoralen may promote callus formation and inhibit osteoclast genesis by increasing BMP‑2 and ER‑α levels, and OPG/RANKL ratio. Consequently, psoralen could be a possible treatment for osteoporotic fracture‑related complications.
To analyze and compare the hidden blood loss of minimally invasive percutaneous plate osteosynthesis(MIPPO) combined with locking plate fixation and intramedullary nail fixation in the treatment of tibial shaft fracture.One hundred and ninety-one cases of tibial shaft fracture treated from January 2017 to January 2019 were analyzed retrospectively. The patients were all treated with closed reduction and divided into two groups:group A (110 cases) and group B (81 cases). In group A, 78 males and 32 females were treated with MIPPO combined with locking plate. The age ranged from 19 to 74 (45.32±11.79) years old. According to AO classification, 42cases were type 42-A, 45 were type 42-B and 23 were type 42-C fractures. Group B was treated with intramedullary nail, including 65 males and 16 females, aged 19 to 84 (45.44± 14.32) years old. According to AO classification, there were 39 cases of type 42-A, 29 cases of type 42-B and 13 cases of type 42-C. The operation time, intraoperative blood loss and hidden blood loss were observed and compared between the two groups.On the first day, the hidden blood loss was (155.27±47.89) ml in group A and (160.43±131.42) ml in group B, the difference was statistically significant (P<0.001);on the third day, the hidden blood loss was (102.70±94.79) ml in group A and (338.23±85.24) ml in group B, the difference was statistically significant (P<0.001). There was no significant difference between the two groups in gender, age, height, weight, fracture type and preoperative Hct (P>0.05).In the treatment of tibial shaft fracture with intramedullary nail, there is obvious hidden blood loss, which is much higher than expected.
The aim of this study was to evaluate the clinical outcome of tibial pilon fractures treated with arthroscopy and assisted reduction with an external fixator.Thirteen patients with tibial pilon fractures underwent assisted reduction for limited lower internal fixation with an external fixator under arthroscopic guidance. The weight-bearing time was decided on the basis of repeat radiography of the tibia 3 months after surgery. Postoperative ankle function was evaluated according to the Mazur scoring system.Healing of fractures was achieved in all cases, with no complications such as severe infection, skin necrosis, or an exposed plate. There were 9 excellent, 2 good, and 2 poor outcomes, scored according to the Mazur system. The acceptance rate was 85%.Arthroscopy and external fixator-assisted reduction for the minimally invasive treatment of tibial pilon fractures not only produced less trauma but also protected the soft tissues and blood supply surrounding the fractures. External fixation could indirectly provide reduction and effective operative space for arthroscopic implantation, especially for AO type B fractures and partial AO type C1 fractures.
Abstract Background: Drynaria Fortunei and Cuscuta Chinensis are among the most used traditional Chinese medicine herbal prescriptions and have a significant therapeutic effect on osteoarthritis. However, the purpose of this study intends to elaborate the molecular mechanism of action through network pharmacology. The active ingredients of TCM and the potential targets for the treatment of osteoarthritis were selected through the TCMSP, OMIM and Genecards. Results: The 27 components and 85/117 targets of Drynaria Fortunei and/or Cuscuta Chinensis were identified for osteoarthritis. Pharmacological and PPI network analysis identified top 3 active components (kaempferol, luteolin, and quercetin) and core proteins (IL6, AKT1, and VEGFA). GO and KEGG analysis identified the top 3 functions (cytokine and cell/nuclear receptor) and pathways (PI3K-Akt, TNF and IL-17). Molecular docking showed strong binding ability between quercetin-AKT1 and luteolin-IL6/VEGFA. Interaction analysis mapped the quercetin-AKT1 and luteolin-IL6/VEGFA binding to specific hydrogen and hydrophobic bonds. Conclusions: The main active components, common target proteins, functional activities, and signaling pathways of TCM Drynaria Fortunei and Cuscuta Chinensis for the treatment of osteoarthritis were identified by Network pharmacology. We found, for the first time, that drynariae rhizoma and cuscuta chinensis suppress osteoarthritis by quercetin-AKT1/IL6 and luteolin-VEGFA direct binding. Our findings have significant implication for our understanding of the molecular mechanism of action in the treatment of osteoarthritis and future development of osteoarthritis treatment using quercetin and luteolin.
Backgroud and Aims: MicroRNA-206 has proven to be down-regulated in many human malignancies in correlation with tumour progression. Our study aimed to characterize miR-206 contributions to initiation and malignant progression of human osteosarcoma. Methods: MiR-206 expression was detected in human osteosarcoma cell 1ine MG63, human normal osteoblastic cell line hFOB 1.19, and paired osteosarcoma and normal adjacent tissues from 65 patients using quantitative RT-PCR. Relationships of miR-206 levels to clinicopathological characteristics were also investigated. Moreover, miR-206 mimics and negative control siRNA were transfected into MG63 cells to observe effects on cell viability, apoptosis, invasion and migration. Results: We found that miR-206 was down-regulated in the osteosarcoma cell line MG63 and primary tumor samples, and decreased miR-206 expression was significantly associated with advanced clinical stage, T classification, metastasis and poor histological differentiation. Additionally, transfection of miR-206 mimics could reduce MG-63 cell viability, promote cell apoptosis, and inhibit cell invasion and migration. Conclusions: These findings indicate that miR-206 may have a key role in osteosarcoma pathogenesis and development. It could serve as a useful biomarker for prediction of osteosarcoma progression, and provide a potential target for gene therapy.
Objective:To introduce the clinical results of repairing bone defects of bone tumor with microsurgery.Methods:A series of 15 cases operated microsurgically on with big bone defects of bone tumor were sumed up retrospectively.Treatment of sick place:Sick place were razed within the cyst in12 cases.Segment of tumor.were cut off in 3 cases.Repairation of bone defects:Transplantation of the vascular pedicled bone falp was in 12 cases.Free vascularized fibular graft was in 3 cases.Results:All of the patients have been followed-up for 6 months to 8 years with average 3.5 years and had a excellent bone union in 3～4 months.None of them had a relapse.Conclusion:It is able to shorten the time of bone union that applying microsurgical techniqe repairs bone defects of bone tumor.The rate of relapse is low.The function of the patients restores well.
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