Acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) has been reported to play an essential role in the development and progression of various human cancers. However, its expression pattern and possible mechanism in human hepatocellular carcinoma (HCC) remain to be elucidated. In this study, we used western blot and immunohistochemical staining to detect protein expression. The effects of ANP32A on the proliferation, migration and invasion of HCC cells were examined using 5-ethynyl-20-deoxyuridine (EdU), colony formation, CCK-8, and transwell assays. RT-qPCR was performed to detect mRNA expression. The interaction between ANP32A and the high mobility group A1 (HMGA1) mRNA was assessed using RNA immunoprecipitation (RIP). The tumorigenicity of ANP32A was assessed by establishing a xenograft tumor model in Balb/c nude mice. We found that the ANP32A protein was expressed at high levels in patients with HCC, which was associated with a poor prognosis. Functional experiments revealed that the silencing of ANP32A inhibited the proliferation, migration, and invasion of HCC cells, whereas overexpression of ANP32A promoted these processes. Further investigations indicated that ANP32A bound the HMGA1 mRNA and maintained its stability to promote the expression of HMGA1, thereby increasing the expression and activation of STAT3. Finally, a xenograft tumor model of Balb/c nude mice confirmed the tumorigenicity of ANP32A. This study found that ANP32A is up-regulated in patients with HCC and may accelerate the proliferation, migration and invasion of HCC cells by modulating the HMGA1/STAT3 pathway.
Rationale: Sjögren syndrome (SS) is a prevalent autoimmune disorder targeting exocrine glands, causing symptoms such as dry eyes and mouth. It often goes underdiagnosed due to its varied presentations, emphasizing the importance of early and accurate diagnosis. Patient concerns: A 22-year-old female presented with atypical symptoms of hypokalemic paralysis and severe bone pain, which are not commonly associated with SS. Diagnoses: Extensive diagnostic workup, including serological tests, ophthalmological assessments, and a lip biopsy, confirmed the diagnosis of distal renal tubular acidosis as a complication of SS. Interventions: The patient was treated with an intensive inpatient regimen designed to stabilize her potassium levels and alleviate her symptoms. Outcomes: The comprehensive therapeutic intervention was successful, with the patient’s symptoms being alleviated within 2 weeks. Lessons: This case underscores the importance of being aware of SS in younger demographics and the necessity for a prompt and multifaceted treatment approach to manage systemic effects and improve quality of life.
Diabetes mellitus complicated with heart failure has high mortality and morbidity, but no reliable diagnoses and treatments are available. This study aimed to develop and verify a new model nomogram based on clinical parameters to predict diastolic cardiac dysfunction in patients with Type 2 diabetes mellitus (T2DM).
Abstract objective Existing studies have confirmed that both high triglyceride(TG) and high non-density lipoprotein cholesterol(Non-HDL-c) are often closely linked with diabetes or insulin resistance. However, there are no large prospective cohort studies that have discussed the connection between the TG/non-HDL-c ratio and incident type 2 diabetes. Therefore, using data from a large cohort representative of the Chinese population, we conducted a retrospective study to explore the relationship between TG/ non-HDL-c ratio and the incidence of type 2 diabetes. Method We did a retrospective study. Participants were comprised of 114,854 Chinese adults, aged over 20 years, distributed in 32 locations and 11 cities in southern China (Shenzhen, Beijing, Wuhan, Shanghai, Nanjing, Suzhou, Chengdu, Guangzhou, Nantong, Hefei, Changzhou); Participants were free of diabetes at baseline. The data included triglyceride (TG), high-density lipoprotein cholesterol (HDL-c), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), age, gender, body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), BUN, serum creatinine (Scr), AST, ALT, smoking and family history of diabetes and drinking status. Non-HDL-c is equal to total cholesterol minus HDL-c. And Cox proportional risk model was used to explore the relationship between TG/ Non-HDL-c and diabetes risk. Generalized additive model (GAM) and smooth curve fitting were used to assess the non-linear relationship between diabetes risk and TG/ non-HDL-c. In addition, subgroup analysis was performed for some variables, such as age, gender, BMI, FPG, ALT, SBP, DBP, smoking status, drinking status, family history. For the record, the data came from the DATADRYAD website. Result Finally, gender, age, systolic blood pressure, systolic blood pressure, FPG, BMI, smoking, family history of diabetes, drinking status, LDL, BUN, ALT, AST and Scr were adjusted, the results showed that TG/non-HDL was positively correlated with the occurrence of diabetes (HR = 1.191 (1.148, 1.235) p < 0.00001). And two-piecewise linear regression model proved a non-linear connection between TG/non-HDL -C and the risk of diabetes, and there was an obvious turning point of 0.544. When the TG/non-HDL-c < 0.544, HR = 5.888 (3.959, 8.757) p < 0.0001, when the TG/non-HDL-c > 0.544, HR = 1.509 (1.056, 2.156) p < 0.0238. The sensitivity analysis confirmed the robustness of the results. Subgroup analysis showed a stronger association among participants who were female, SBP < 140mmhg, FPG < 6.1 mmol/L, BMI (≥ 18.5, < 24kg/m 2), and never drinking status. Conclusion Increased TG/non-HDL-c was independently and nonlinearly associated with the occurrence of diabetes, and the correlation was stronger when the TG/non-HDL-C < 0.544.
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