The traditional herbal medicinal product Myrrhinil-Intest®, a combination of myrrh, chamomile flower and coffee charcoal is used for the relief of gastrointestinal complaints. Clinical studies suggest its use in the maintenance therapy of inflammatory bowel disease [1]. However, the pharmacological mechanisms underlying the clinical effects are not yet fully understood. The present study aims to elucidate immunopharmacological activities of myrrh, chamomile flower and coffee charcoal by studying their influence on gene expression and protein release of activated human macrophages. The effect of ethanolic myrrh (MY), chamomile flower (KA) and coffee charcoal extract (CC) on gene and protein expression of activated human monocyte-derived macrophages was investigated by microarray gene expression analysis and assessment of the release of pro- and anti-inflammatory mediators (TNFα, chemokine CXCL13 and IL10) using an ELISA test system.
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