Alizée Guérin

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

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Co-Authors

Véronique Broussolle

Université d'Avignon et des Pays de Vaucluse

Thierry Clavel

Sécurité et Qualité des Produits d'Origine Végétale

Claire Dargaignaratz

Sécurité et Qualité des Produits d'Origine Végétale

C. Nguyen‐The

Sécurité et Qualité des Produits d'Origine Végétale

Odile Tresse

PhysioPathologie des Adaptations Nutritionnelles

Christophe Soumet

Centre Hospitalier de Fougères

Amélie Rouger

Université Nantes Angers Le Mans

Yann Sévellec

Paris-Est Sup

Féderica Palma

Université Paris Cité

Benjamin Félix

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Cooperative Institutions

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Oniris

Institut National de la Recherche Agronomique

Paris-Est Sup

Agence Nationale des Fréquences

Sécurité des Aliments et Microbiologie

Sécurité et Qualité des Produits d'Origine Végétale

Centre National de la Recherche Scientifique

AgroParisTech

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Impact of temperature, pH and oxygen on the fate of Bacillus cereus vegetative cells. Consequences on the safety of new food products for elderly

HAL (Le Centre pour la Communication Scientifique Directe) (2014)

Alizée Guérin Claire Dargaignaratz Véronique Broussolle Thierry Clavel

Impact of temperature, pH and oxygen on the fate of [i]Bacillus cereus[/i] vegetative cells. Consequences on the safety of new food products for elderly. FoodMicro 2014

Cereus

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Citations (0)

Membrane Proteocomplexome of Campylobacter jejuni Using 2-D Blue Native/SDS-PAGE Combined to Bioinformatics Analysis

Frontiers in Microbiology (2020)

Alizée Guérin Sheiam Sulaeman Laurent Coquet Armelle Ménard Frédérique Barloy-Hubler

Campylobacter is the leading cause of the human bacterial foodborne infections in the developed countries. The perception cues from biotic or abiotic environments by the bacteria are often related to bacterial surface and membrane proteins that mediate the cellular response for the adaptation of Campylobacter jejuni to the environment. These proteins function rarely as a unique entity, they are often organized in functional complexes. In C. jejuni , these complexes are not fully identified and some of them remain unknown. To identify putative functional multi-subunit entities at the membrane subproteome level of C. jejuni , a holistic non a priori method was addressed using two-dimensional blue native/Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) in strain C. jejuni 81–176. Couples of acrylamide gradient/migration-time, membrane detergent concentration and hand-made strips were optimized to obtain reproducible extraction and separation of intact membrane protein complexes (MPCs). The MPCs were subsequently denatured using SDS-PAGE and each spot from each MPCs was identified by mass spectrometry. Altogether, 21 MPCs could be detected including multi homo-oligomeric and multi hetero-oligomeric complexes distributed in both inner and outer membranes. The function, the conservation and the regulation of the MPCs across C. jejuni strains were inspected by functional and genomic comparison analyses. In this study, relatedness between subunits of two efflux pumps, CmeABC and MacABputC was observed. In addition, a consensus sequence CosR-binding box in promoter regions of MacABputC was present in C. jejuni but not in Campylobacter coli . The MPCs identified in C. jejuni 81–176 membrane are involved in protein folding, molecule trafficking, oxidative phosphorylation, membrane structuration, peptidoglycan biosynthesis, motility and chemotaxis, stress signaling, efflux pumps and virulence.

Proteome

Sodium dodecyl sulfate

Efflux

10.3389/fmicb.2020.530906

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Citations (3)

Behavior of the pathogenic bacterium Bacillus cereus in ready for use food - Impact of the physico-chemical conditions

HAL (Le Centre pour la Communication Scientifique Directe) (2016)

Alizée Guérin

Pathogenic bacteria

Cereus

Bacillus (shape)

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Advanced 'omics approaches applied to microbial food safety & quality : from ecosystems to the emerging foodborne pathogen Campylobacter

HAL (Le Centre pour la Communication Scientifique Directe) (2019)

Alizée Guérin Amélie Rouger Raouf Tareb Jenni Hultman Johanna Björkroth

Foodborne pathogen

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Impact of temperature and oxygen on the fate of Bacillus weihenstephanensis in a food-based medium

Food Microbiology (2019)

Alizée Guérin Claire Dargaignaratz Thierry Clavel Véronique Broussolle C. Nguyen‐The

Cereus

10.1016/j.fm.2019.05.011

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Citations (1)

Behaviour of psychrotrophic Bacillus cereus during the life cycle of food products for elderly people

HAL (Le Centre pour la Communication Scientifique Directe) (2016)

Alizée Guérin Claire Dargaignaratz Véronique Broussolle Thierry Clavel

The aim of the FP7-OPTIFEL project is to create new food products based on fruits and vegetables, supplemented with specific nutrients to fight elderlies’ nutritional deficiencies. The microbial safety of these products is of great importance due to the high risk of foodborne disease in the elderly population. The foodborne pathogen Bacillus cereus can resist to pasteurization treatments, psychrotrophic strains can multiply during food storage at low temperatures, and some may produce the heat resistant emetic toxin cereulide.

Pasteurization

Food Products

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Exposure to Quaternary Ammonium Compounds Selects Resistance to Ciprofloxacin in Listeria monocytogenes

Pathogens (2021)

Alizée Guérin Arnaud Bridier Patricia Le Grandois Yann Sévellec Féderica Palma

In this contribution, the antimicrobial susceptibility toward 11 antibiotics and four biocides of a panel of 205 Listeria monocytogenes (Lm) strains isolated from different ecological niches (i.e., food, animals and natural environment) was evaluated. The impact of exposure to biocides on the antibiotic susceptibilities of Lm was also investigated. Lm strains isolated from food exhibited overall a lower susceptibility (higher minimal inhibitory concentrations, MIC) for ammonium quaternary compounds (QACs) and peracetic acid (PAC) than strains isolated from animals and natural environments. Conversely, the ecological origins of Lm strains did not significantly affect their susceptibilities towards antibiotics. Interestingly, repeated exposure to QACs recurrently led to a decrease in susceptibility toward ciprofloxacin (CIP), a fluoroquinolone antibiotic, largely used in human medicine. Moreover, these lower levels of susceptibility to CIP remained stable in most Lm strains even after subcultures without biocide selection pressure, suggesting an adaptation involving modifications at the genetic level. Results underlined the ability of Lm to adapt to biocides, especially QACs, and the potential link between this adaptation and the selection of resistance toward critical antibiotics such as ciprofloxacin. These data support a potential role of the extensive use of QACs from “farm to fork” in the selection of biocide and antibiotic resistance in pathogenic bacteria such as Lm.

Biocide

10.3390/pathogens10020220

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Citations (39)

Genomic elements located in the accessory repertoire drive the adaptation to biocides in Listeria monocytogenes strains from different ecological niches

Food Microbiology (2021)

Féderica Palma Nicolas Radomski Alizée Guérin Yann Sévellec Benjamin Félix

Biocide

Benzalkonium chloride

Lineage (genetic)

Vibrio vulnificus

Prophage