Objective To study the effectiveness of sterilized absorbing-sputum tube by fog-fumigating and dipping sterilization.Methods During a period of 12 days, the used absorbing sputum tubes were randomly into 2 groups after basic disposal (dipping in 5% 84 sterilizing liquid for 30 min and then washing). The tubes in the tested group were sterilized by fog-fumigating for 4 h and those in the control group by dipping sterilization for 30 min. The samples of absorbing-sputum tubes and the fluid during sterilization were obtained at the first day, 6th day and 12th day separately, numbering bacteria were observed.Results On the first and 6th day during each period, bacteria were not detected in the samples of absorbing-sputum tubes and sterilized liquid. On the 12th day in each period, bacteria were detectable in the control group, but not detectable in the tested group. Conclusion Fog-fumigating for sterilization of absorbing-sputum tubes is economic, convenient, and is easy to deposit and save in comparing to dipping sterilization.
Abstract Objective This study aimed to investigate the characteristic of brain structural connections in glioma patients and further evaluate the relationship between changes in the white matter tracts and cognitive decline. Methods This retrospective study included a total of 35 subjects with glioma and 14 demographically matched healthy controls, who underwent diffusion tensor imaging scans and formal neuropsychological assessment tests. Fractional anisotropy (FA) values of white matter tracts were derived from atlas‐based analysis to compare group differences. Furthermore, subgroup‐level analysis was performed to differentiate the effects of tumor location on white matter tracts. Partial correlation analysis was used to examine the associations between neurocognitive assessments and the integrity of tracts. Region of interest‐based network analysis was performed to validate the alteration of structural brain network in subjects with glioma. Results Compared with controls, subjects with glioma exhibited reduced FA values in the right uncinate fasciculus. Besides, subjects with glioma exhibited worse performance in several cognitive assessments. Partial correlation analysis indicated that the FA value in the right superior longitudinal fasciculus temporal part was significantly positively correlated with scores of visual–spatial abilities in subjects with glioma in the right temporal lobe ( r = .932, p = .002). Region of interest‐based network analysis revealed that subjects with glioma exhibited reduced FA, fiber length (FL), and fiber number (FN) between specific brain regions compared with controls. Conclusion The present study demonstrated the reduced integrity of white matter tracts and altered structural connectivity in brain networks in patients with glioma. Notably, white matter tracts in the right hemisphere might be vulnerable to the effects of a frontal or temporal lesion and might be associated with deficient cognitive function.
Introduction: Dynamins (Dnm) and Dnm related protein 1 (DRP1) play central roles in cell biology by regulating pathways involved in endocytosis and mitochondrial fission, respectively. Disruption of both processes has been implicated in cardiac dysfunction associated with ischemia reperfusion (IR) injury. Recently, the small noncompetitive Dnm GTPase inhibitor, Dynasore (Dyn) was shown to improve cardiac lusitropy in response to IR injury. Here, we investigated the electrophysiological (EP) consequences of Dyn under conditions of acute oxidative stress (OS) caused by H2O2 challenge or IR injury. Methods: Rat hearts (N=39) were treated with a low (1uM) or high (5uM) concentration of Dyn for 35 or 120 min before challenge with H2O2 (200uM) or no flow ischemia for 12 min followed by reperfusion. High resolution optical mapping was used to measure spatio-temporal changes in the action potential and mitochondrial membrane potential (MMP) across the heart. Results: Pretreatment of hearts with 1um Dyn for 120 min but not 35 min suppressed the incidence of reperfusion arrhythmias compared to untreated hearts (5/12 vs 6/7). Investigation of the EP substrate revealed marked attenuation of conduction slowing with no change in APD in response to ischemia in Dyn treated hearts. These beneficial effects were abrogated by selective PKC inhibition in an additional subset of experiments (n=3). Similar to IR injury, challenge of hearts with H2O2 for 30 min was associated with a significantly (p=0.0019) lower arrhythmia score in Dyn-treated compared to untreated hearts. Underlying this reduction in arrhythmic vulnerability was the prevention of H2O2 induced MMP depolarization and attenuation of MMP heterogeneity. Importantly, treatment of hearts with a higher (5uM) concentration of Dyn that is more consistent with inhibition of clatherin mediated endocytosis resulted in the rapid elevation of coronary perfusion pressure, MMP depolarization, and early onset of arrhythmias. Conclusions: At low but not high concentrations, Dyn exerts potent anti-arrhythmic activity likely by preventing OS-induced mitochondrial fission and associated dysfunction. Selective DRP1 inhibition may be a novel strategy for arrhythmia suppression in ischemic heart disease.
To evaluate the efficacy and safety of pelvic arterial embolization (PAE) in women with intractable primary postpartum hemorrhage (PPH).Clinical data of 36 cases were analyzed retrospectively in which women underwent PAE for intractable primary PPH in Peking Union Medical College Hospital between Jan 2006 and Jan 2015. The success rate of PAE were measured and possible predictive risk factors associated with treatment failure were analyzed. The complications secondary to PAE were also recorded.(1) The etiology of PPH. Among the 36 cases, 21 patients delivered viginally (Group VD) and 15 received cesarean section (Group CS). The most frequent cause of PPH was uterine atony (72%, 26/36). The less common causes were placental problems (28%, 10/36), genital tract trauma (6%, 2/36) and coagulation defects (3%, 1/36) in turn. Three patients (8%, 3/36) had combined causes. (2) Interventions before PAE. Uterotonic medications were used in all patients. 31 patients received carboprost methylate suppositorites, 27 received carbetocin and 31 received carboprost tromethamine. Besides, 20 patients received one or more surgical interventions before PAE. PAE was performed when these interventions failed. (3) Characteristics of PAE. Altogether 78 arteries were embolized in 36 cases. Embolization of bilateral uterine arteries was performed in 31 cases, right internal iliac artery and bilateral inferior epigastric arteries were embolized in one case. Right internal pudendal artery, bilateral uterine arteries and bilateral internal iliac arteries were embolized in one case. And bilateral uterine arteries, bilateral internal iliac arteries were embolized in one case. In the other 2 cases, bilateral internal iliac arteries were embolized. (4) Efficacy of PAE. The overall technical success rate of PAE was 100%(36/36), while the clinical success rate was 94%(34/36). All patients survived. (5) Complications of PAE. 15 patients were transferred to ICU after PAE for 1 to 7 days. Except self-limited fever, no puncture site hematoma, buttock necrosis or vessel rupture was observed. The effect on menstrual cycle and fertility were followed in 25 patients. 17 (68%, 17/25) reported resumption of normal menses and 8 (32%, 8/25) reported amenorrhea. Three pregnancies after PAE were observed.PAE is a safe and effective treatment for intractable primary PPH which can prevent hysterectomy and preserve fertility of patients.
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