ТК

Т. К. Махина

Russian Academy of Sciences

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ГА

Г. А. Бонарцева

Federal Center Research Fundamentals of Biotechnology

АП

А. П. Бонарцев

Lomonosov Moscow State University

ВЛ

В. Л. Мышкина

Russian Academy of Sciences

КВ

К. В. Шайтан

Lomonosov Moscow State University

ВВ

В. В. Воинова

Lomonosov Moscow State University

ВА

В. А. Жуйков

Russian Academy of Sciences

ИИ

И. И. Жаркова

Lomonosov Moscow State University

ДВ

Д. В. Чеснокова

Lomonosov Moscow State University

Sergey Yakovlev

Moscow State Pedagogical University

Elizaveta Akoulina

Lomonosov Moscow State University

Cooperative Institutions

Lomonosov Moscow State University

Russian Academy of Sciences

Moscow State University

A N Bach Institute of Biochemistry

National Research Tomsk State University

Tomsk Polytechnic University

Federal Center Research Fundamentals of Biotechnology

Privolzhsky Research Medical University

Semenov Institute of Chemical Physics

Peoples' Friendship University of Russia

Author Statistics

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The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro

Polymers (2020)

В. А. Жуйков Elizaveta Akoulina Д. В. Чеснокова Wenhao You Т. К. Махина

Over the past century there was a significant development and extensive application of biodegradable and biocompatible polymers for their biomedical applications. This research investigates the dynamic change in properties of biodegradable polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), and their 50:50 blend (PHB/PLA)) during their hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) and enzymatic degradation (in PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on the polymer films experiencing different degradation durations was also studied. Enzymatic degradation significantly accelerated the degradation rate of polymers compared to non-enzymatic hydrolytic degradation, whereas the seeding of 3T3 cells on the polymer films accelerated only the PLA molecular weight loss. Surprisingly, the immiscible nature of PHB/PLA blend (showed by differential scanning calorimetry) led to a slower and more uniform enzymatic degradation in comparison with pure polymers, PHB and PLA, which displayed a two-stage degradation process. PHB/PLA blend also displayed relatively stable cell viability on films upon exposure to degradation of different durations, which was associated with the uneven distribution of cells on polymer films. Thus, the obtained data are of great benefit for designing biodegradable scaffolds based on polymer blends for tissue engineering.

Biodegradable polymer

Degradation

Polymer degradation

10.3390/polym13010108

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Citations (32)

Growth of mesenchymal stem cells on oriented microstructured films and electrospun scaffolds

Vestnik Moskovskogo universiteta Seria 16 Biologia (2023)

I. V. Demianova Elizaveta Akoulina И. И. Жаркова V.V. Voinova Д. В. Чеснокова

The study involved the fabrication of films with different roughness and scaffolds made of poly(3-hydroxybutyrate) using various methods. Chaotic and oriented scaffolds with varying fiber thickness were obtained through the electrospinning method, depending on the polymer concentration and electrospinning parameters. Films with different surface roughness were obtained using spin coating and self-assembly methods. It was demonstrated that the varying microstructure of the surface does not affect the growth of mesenchymal stem cells over the course of a week; however, it does influence the morphology of the adhered cells.

Electrospinning

Spin Coating

10.55959/10.55959/msu0137-0952-16-78-3s-6

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Citations (1)

Surface Modification of Ti-Nb-Zr Foams by Poly(3-Hydroxybutyrate)

Materials research proceedings (2018)

Yulia Zhukova Г. А. Бонарцева Т. К. Махина E.А. Akulina Е. В. Иванова

Surface Modification of Ti-Nb-Zr Foams by Poly(3-Hydroxybutyrate) Vadim A. Sheremetyev, Anton P. Bonartsev, Sergey M. Dubinskiy, Yulia S. Zhukova, Garina A. Bonartseva, Tatiana K. Makhina, Elizaveta A. Akoulina, Elina V. Ivanova, Maria S. Kotlyarova, Sergey D. Prokoshkin, Vladimir Brailovski, Konstantin V. Shaitan Abstract In this study, Ti-Nb-Zr superelastic foams were produced, characterized from the standpoint […]

Surface Modification

10.21741/9781644900017-15

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Citations (2)

ПРОЛОНГИРОВАННОЕ ВЫСВОБОЖДЕНИЕ ХЛОРАМБУЦИЛА И ЭТОПОЗИДА ИЗ ПОЛИМЕРНЫХ МИКРОСФЕР НА ОСНОВЕ ПОЛИ-3-ОКСИБУТИРАТА

Прикладная биохимия и микробиология (2012)

А. П. Бонарцев Г. А. Бонарцева Т. К. Махина В. Л. Мышкина Е. В. Филатова

Получены микросферы на основе поли-3-оксибутирата (ПОБ) с включением в полимерную матрицу цитостатических лекарственных веществ (ЛВ) хлорамбуцила и этопозида, изучены морфология, кинетика высвобождения ЛВ из микросфер и взаимодействие микросфер с опухолевыми клетками рака молочной железы человека линии MFC-7 in vitro. Полученные данные свидетельствуют о том, что пролонгированное высвобождение ЛВ происходит за счет диффузии лекарственного вещества из полимерной матрицы на начальном этапе и за счет гидролитической деструкции полимера на более поздних этапах. При изучении биосовместимости и биологической активности биополимерных микросфер показано, что хлорамбуцил сильнее подавляет рост культивируемых опухолевых клеток за короткое время (24 ч). Этопозид действует слабее (подавление роста клеток за 48 ч не превышает 50%), однако в дальнейшем он имеет долгий пролонгированный эффект высвобождения ЛВ из полимерной матрицы. Изучаемая система может послужить основой создания новых лекарственных форм пролонгированного действия этопозида и хлорамбуцила для терапии онкозаболеваний.

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Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect

Materials Science and Engineering C (2020)

А. В. Волков А.А. Мураев И. И. Жаркова В. В. Воинова Elizaveta Akoulina

Biomaterial

Biodegradable polymer

10.1016/j.msec.2020.110991

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Citations (64)

Prolonged release of chlorambucil and etoposide from poly-3-oxybutyrate-based microspheres

Applied Biochemistry and Microbiology (2012)

Е. В. Филатова Sergey Yakovlev А. П. Бонарцев Т. К. Махина В. Л. Мышкина

Chlorambucil

10.1134/s000368381206004x

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Citations (7)

Cell attachment on poly(3-hydroxybutyrate)-poly(ethylene glycol) copolymer produced by Azotobacter chroococcum 7B

BMC Biochemistry (2013)

А. П. Бонарцев Sergey Yakovlev И. И. Жаркова Arasha Boskhomdzhiev Dmitry V. Bagrov

The improvement of biomedical properties, e.g. biocompatibility, of poly(3-hydroxyalkanoates) (PHAs) by copolymerization is a promising trend in bioengineering. We used strain Azotobacter chroococcum 7B, an effective producer of PHAs, for biosynthesis of not only poly(3-hydroxybutyrate) (PHB) and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also alternative copolymer, poly(3-hydroxybutyrate)-poly(ethylene glycol) (PHB-PEG). In biosynthesis we used sucrose as the primary carbon source and valeric acid or poly(ethylene glycol) 300 (PEG 300) as additional carbon sources. The chemical structure of PHB-PEG and PHB-HV was confirmed by 1H nuclear-magnetic resonance (1H NMR) analysis. The physico-chemical properties (molecular weight, crystallinity, hydrophilicity, surface energy) and surface morphology of films from PHB copolymers were studied. To study copolymers biocompatibility in vitro the protein adsorption and COS-1 fibroblasts growth on biopolymer films by XTT assay were analyzed. Both copolymers had changed physico-chemical properties compared to PHB homopolymer: PHB-HV and PHB-PEG had less crystallinity than PHB; PHB-HV was more hydrophobic than PHB in contrast to PHB-PEG appeared to have greater hydrophilicity than PHB; whereas the morphology of polymer films did not differ significantly. The protein adsorption to PHB-PEG was greater and more uniform than to PHB and PHB-PEG copolymer promoted better growth of COS-1 fibroblasts compared with PHB homopolymer. Thus, despite low EG-monomers content in bacterial origin PHB-PEG copolymer, this polymer demonstrated significant improvement in biocompatibility in contrast to PHB and PHB-HV copolymers, which may be coupled with increased protein adsorption and hydrophilicity of PEG-containing copolymer.

Biocompatibility

Protein Adsorption

Azotobacter chroococcum

Polyhydroxybutyrate

Biopolymer

Azotobacter vinelandii

10.1186/1471-2091-14-12

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Citations (57)

[The biodegradation of poly-beta-hydroxybutyrate (PHB) by a model soil community: the effect of cultivation conditions on the degradation rate and the physicochemical characteristics of PHB].

Microbiology (2002)

Г. А. Бонарцева В. Л. Мышкина D. А. Nikolaeva А. В. Ребров В. А. Герасин