Giuliano Gazzotti

Research Field

Base (topology)

Ganglioside

10.1016/s0021-9673(01)90758-7

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Citations (9)

NUCLEAR MONOOXYGENASES: KINETICS OF IN VITRO FORMATION OF STYRENE-7, 8-OXIDE USING STYRENE AS SUBSTRATE

Elsevier eBooks (1978)

Giuliano Gazzotti Mario Salmona

Styrene oxide

10.1016/b978-0-08-023768-8.51702-3

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Citations (0)

High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous long chain base composition.

Journal of Lipid Research (1984)

Sandro Sonnino Riccardo Ghidoni Giuliano Gazzotti G. Kirschner G Galli

A semi-preparative, analytical high performance liquid chromatographic (HPLC) procedure is described for the isolation of molecular species of GMl and GDla gangliosides containing a single long chain base, C18 or C20 sphingosine, C18 or C20 sphinganine, each in its natural erythro or unnatural threo form.T h e threo forms were obtained from 2,3-dichloro-5,6-dicyanobenzoquinone/NaBH4-treated gangliosides.T h e ganglioside molecular species separated by HPLC were analyzed for carbohydrate, fatty acid, and long chain base composition.In particular, long chain bases were submitted to gas-liquid chromatographic-mass spectrometric analyses as their trimethylsilyl (TMS) or N-acetyl-TMS derivatives, and chain length, presence or absence of C4-C5 double bond, and C-3 steric configuration were ascertained.The final preparations of individual molecular species of GMl and GDla gangliosides were more than 99% homogeneous in their saccharide moiety, contained a single long chain base (homogeneity higher than 99%), and had a fatty acid composition primarily of stearic acid (92 to 97%).All the individual molecular species of GM 1 and GDla gangliosides were also prepared in radioactive form by selective tritiation at C-3 of the long chain base.Their specific radioactivity ranged from 1.3 to 1.45 Ci/mmol.The availability of these molecular species of gangliosides is expected to facilitate studies aimed at ascertaining the role played by the hydrophobic portion in the functional behavior of gangliosides.-

Base (topology)

10.1016/s0022-2275(20)37775-0

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Citations (115)

Improved gas chromatographic method for measuring phenylethylene glycol

Journal of Chromatography A (1980)

Giuliano Gazzotti Enrico Garattini Mario Salmona

10.1016/s0021-9673(00)81263-7

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Citations (21)

Perinatal development of styrene monooxygenase and epoxide hydrolase in rat liver microsomes and nuclei

Chemico-Biological Interactions (1983)