Lingling Wu

Chinese Academy of Medical Sciences & Peking Union Medical College

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Hai‐Yu Hu

Chinese Academy of Medical Sciences & Peking Union Medical College

Zhen Wang

Nanjing University of Posts and Telecommunications

Kaiqiang Li

China Pharmaceutical University

Qin Li

Changchun Institute of Optics, Fine Mechanics and Physics

Weide Xu

Wenzhou Medical University

Qinghua Wang

Chinese Academy of Medical Sciences & Peking Union Medical College

Ke Hao

Hangzhou Medical College

Yue Chen

Hunan Academy of Agricultural Sciences

Yaoqiang Du

Zhejiang Provincial People's Hospital

Qingyang Zhang

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Wenzhou Medical University

Chinese Academy of Sciences

Hangzhou Medical College

Zhejiang Provincial People's Hospital

Nankai University

Shandong University

First Affiliated Hospital of Wenzhou Medical University

Sichuan University

Zhejiang University

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A self-immobilizing near-infrared fluorogenic probe for in vivo imaging of fibroblast activation protein-α

Talanta (2024)

Yongyi Chen Jiacheng Du Xiangchuan Meng Lingling Wu Qingyang Zhang

10.1016/j.talanta.2024.126475

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Citations (0)

Understanding precipitation concentration changes, driving factors, and responses to global warming across mainland China

Journal of Hydrology (2024)

Juan Du Li Zhou Xiaojing Yu Yibo Ding Yunkai Zhang

10.1016/j.jhydrol.2024.132164

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Citations (1)

Syntheses and anti-pancreatic cancer activities of rakicidin A analogues

European Journal of Medicinal Chemistry (2018)

Jian Chen Jingpei Li Lingling Wu Yan Geng Jianming Yu

IC50

10.1016/j.ejmech.2018.03.078

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Citations (16)

Platelet–Tumor Cell Hybrid Membrane-Camouflaged Nanoparticles for Enhancing Therapy Efficacy in Glioma

Research Square (Research Square) (2021)

Lingling Wu Qin Li Junjie Deng Weide Xu Bing‐Yu Chen

Abstract Cell membrane-camouflaged nanoparticles are drawing increasing attention because their surfaces retain the natural functionalities of the cell plasma membranes, making them a unique class of biomimetic materials combining natural and synthetic components. Modifying the cell membranes or combining the functions of different types of membranes enhances their functionality. Herein, we prepared platelet and tumor cell membrane camouflaged antitumor nanoparticles. The effects of β-mangostin-loaded nanoparticles on the target and its anticancer action in glioma were measured in vitro and in vivo. Multifunctional nanoparticles were manufactured with platelet–C6 hybrid biomimetic coating (PCM), lactic-co-glycolic acid (PLGA), and β-mangostin. PCM increased the proportion of active drug targeting in C6 and immune escape characteristics in THP-1 cells, thus enhancing the cytotoxicity of β-PCNPs. The β-PCNPs were comprehensively characterized to study the inherent properties of both source cells. Compared with bare β-NPs, β-PCNPs exhibited high tumor-targeting ability and induced apoptosis of C6 cells in vitro. Mice experiments with intravenous administration of the drug revealed that the β-PCNP platform enhanced the tumor targeting capability and exhibited excellent chemotherapy with high inhibition rate of glioma tumor growth in vivo. The mice in the β-PCNP group had a markedly prolonged circulation lifetime and exhibited better outcome than those in the β-NP group. These results provide a new strategy of utilizing PCNPs as carriers for drug delivery, which improves the targeting efficiency and therapeutic efficacy of chemotherapeutic agents for glioma therapy.

PLGA

10.21203/rs.3.rs-664514/v1

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C21 Fraction Refined from Marsdenia tenacissima-Induced Apoptosis is Enhanced by Suppression of Autophagy in Human Gastric Cell Lines

ACS Omega (2020)

Kaiqiang Li Ke Hao Yu Zhang Aibo Xu Qianni Wang

C21 steroidal glycosides have been extensively reported for treating several types of cancer and are widely found in Marsdenia tenacissima. In this study, a C21 fraction was synthesized from M. tenacissima, and its anti-cancer potency was assessed against in vitro gastric cell lines BGC-823, SGC-7901, and AGS. Significant growth inhibition and cell cycle arrest were observed in C21 fraction-treated gastric cancer cells. The results of apoptotic staining techniques in C21 fraction-treated gastric cells were confirmed with excess reactive oxygen species generation. Moreover, SOD and H2O2 levels were increased by C21 fraction, especially when combined with chloroquine (CQ). The apoptotic inducing potential of C21 fraction was also evidenced by upregulation of proapoptotic proteins cleaved-PARP and BAX and downregulation of antiapoptotic proteins Bcl-2 and p-AKT by western blot, especially in the presence of the autophagy inhibitor CQ. The results showed that the apoptosis of gastric cancer cells caused by C21 fraction was enhanced by inhibiting autophagy. The current findings reveal a new mechanism for the antitumor activity of C21 fraction on gastric cancer.

10.1021/acsomega.0c02748

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Rapid differentiation between bacterial infections and cancer using a near-infrared fluorogenic probe

Chemical Science (2020)

Lingling Wu Qinghua Wang Yali Wang Na Zhang Qingyang Zhang

A new nitroreductase-responsive near-infrared fluorogenic probe can specifically image live bacteria in mouse models and does not accumulate at sites of inflammation or tumor.

Nitroreductase

10.1039/d0sc00508h

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Citations (39)

Polymyxin-based fluorescent probes to combat Gram-negative antimicrobial resistance

Talanta (2023)

Xiaowan Han A-Long Cui He-Xian Yang Lingling Wu Rao Wei

Gram-Negative Bacteria

Polymyxin

Polymyxin B

Gram-Positive Bacteria

Gram

10.1016/j.talanta.2023.124576

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Citations (1)

Neuroprotective effects of ulinastatin on Escherichia coli meningitis rats through inhibiting PKCα phosphorylation and reducing zonula occludens-1 degradation

Scientific Reports (2024)

Xiaolan Zheng Junsheng Wang Huihong Su Lingling Wu Yan Zhang

Ulinastatin

Degradation

10.1038/s41598-024-72097-5

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Citations (1)

E2F-Rb Complexes Assemble and Inhibit cdc25A Transcription in Cervical Carcinoma Cells following Repression of Human Papillomavirus Oncogene Expression

Molecular and Cellular Biology (2000)

Lingling Wu Edward C. Goodwin Lisa K. Naeger Elena Vigo Konstantin Galaktionov

Expression of the bovine papillomavirus E2 protein in cervical carcinoma cells represses expression of integrated human papillomavirus (HPV) E6/E7 oncogenes, followed by repression of the cdc25A gene and other cellular genes required for cell cycle progression, resulting in dramatic growth arrest.To explore the mechanism of repression of cell cycle genes in cervical carcinoma cells following E6/E7 repression, we analyzed regulation of the cdc25A promoter, which contains two consensus E2F binding sites and a consensus E2 binding site.The wild-type E2 protein inhibited expression of a luciferase gene linked to the cdc25A promoter in HT-3 cervical carcinoma cells.Mutation of the distal E2F binding site in the cdc25A promoter abolished E2-induced repression, whereas mutation of the proximal E2F site or the E2 site had no effect.None of these mutations affected the activity of the promoter in the absence of E2 expression.Expression of the E2 protein also led to posttranscriptional increase in the level of E2F4, p105 Rb , and p130 and induced the formation of nuclear E2F4-p130 and E2F4-p105 Rb complexes.This resulted in marked rearrangement of the protein complexes that formed at the distal E2F site in the cdc25A promoter, including the replacement of free E2F complexes with E2F4-p105 Rb complexes.These experiments indicated that repression of E2F-responsive promoters following HPV E6/E7 repression was mediated by activation of the Rb tumor suppressor pathway and the assembly of repressing E2F4-Rb DNA binding complexes.Importantly, these experiments revealed that HPV-induced alterations in E2F transcription complexes that occur during cervical carcinogenesis are reversed by repression of HPV E6/E7 expression.

E2F

10.1128/mcb.20.19.7059-7067.2000

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Citations (52)

Total Synthesis and Determination of the Absolute Configuration of Rakicidin A

Journal of the American Chemical Society (2014)

Feng Sang Dongmei Li Xiaolong Sun Xianqiang Cao Liang Wang

Rakicidin A is a cyclic depsipeptide that has exhibited unique growth inhibitory activity against chronic myelogenous leukemia stem cells. Furthermore, rakicidin A has five chiral centers with unknown stereochemical assignment, and thus, can be represented by one of 32 possible stereoisomers. To predict the most probable stereochemistry of rakicidin A, calculations and structural comparison with natural cyclic depsipeptides were applied. A total synthesis of the proposed structure was subsequently completed and highlighted by the creation of a sterically hindered ester bond (C1-C15) through trans-acylation from an easily established isomer (C1-C13). The analytic data of the synthetic target were consistent with that of natural rakicidin A, and then the absolute configuration of rakicidin A was assigned as 2S, 3S, 14S, 15S, 16R. This work suggests strategies for the determination of unknown chiral centers in other cyclic depsipeptides, such as rakicidin B, C, D, BE-43547, and vinylamycin, and facilitates the investigations of rakicidin A as an anticancer stem cell agent.

Depsipeptide

Absolute Configuration

10.1021/ja509379j

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Citations (40)