Zhejiang has rich resources of wild fruit trees.Aiming at low utilization rate and lack of diversity of wild fruit trees resources in urban garden green land,according to using requirement and environmental character of each type of urban garden green land,the study statistically analyses Zhejiang wild fruit tree species which are suitable for being planted in each type of urban garden green land,such as park green land,protective green land,attached green land and others,and puts forward existing problems in application and some countermeasures.
ABSTRACT Light is one of the most important environmental factors that affect plant growth and development. It also stimulates anthocyanin biosynthesis in plants. However, the precise molecular mechanisms through which light regulates anthocyanin biosynthesis, particularly in non‐model plant species, remain poorly understood. In this study, we discovered a CmBBX28‐CmMYB9a molecular module that is responsive to light and regulates anthocyanin biosynthesis in chrysanthemums. Specifically, CmBBX28 interacts with CmMYB9a, interfering with its binding to the promoters of target genes and reducing the protein abundance of CmMYB9a. This interaction downregulates the transcription of CmMYB9a's downstream anthocyanin‐associated genes, CmCHS , CmDFR , and CmUFGT . The expression of CmBBX28 was induced in the dark, and the accumulated CmBBX28 proteins interfered with the activation of CmMYB9a during anthocyanin biosynthesis. Concurrently, darkness also inhibited the expression of CmMYB9a to some extent. In contrast, light significantly induced the expression of CmMYB9a and suppressed the expression of CmBBX28 , resulting in increased anthocyanin accumulation in chrysanthemum petals. Our findings reveal the mechanism by which light regulates anthocyanin biosynthesis in chrysanthemum flower petals.
Based on analysis of the status of Fuquanshan Forest Park,by using the method of landscape design and ecological theory,this paper propose that Fuquanshan Forest Park will be built a provincial level forest park,with the functions of sightseeing tour,vacations,science education,with the characteristics of the Taoist,medicine and reclusive culture.
Circular RNAs(circRNA) are recently demonstrated to have a close relationship with tumors.To investigate the role of circular RNA in the pathogenesis of salivary adenoid cystic carcinoma(SACC), ten SACC tissues and paired normal submandibular gland(SMG) tissues were collected as the tumor group and the control group. Total RNA was extracted and then measured using ceRNA microarray (including mRNA, lncRNA, and circRNA) and miRNA microarray. Gene Ontology(GO) analysis and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway analysis were performed in order to investigate the function of the differential expressing genes. The ceRNA regulatory network was constructed to find the core circRNAs. Then the role of circRNA on proliferation was examined in the SACC cell line SACC-83 using CCK-8,qRT-PCR and western blotting, and its roles on migration and invasion were examined using wound healing assay and transwell assay. The results of the microarrays showed that 3792 mRNAs, 7649 lncRNAs, 11553 circRNAs, and 132 miRNAs expressed differentially. The ceRNA regulatory network analysis showed that hsa_circ_0059655 and other 14circRNAs derived from PYGB target on several similar genes by miR-338-3p.Among the 15 circRNAs derived from PYGB, hsa_circ_0059655has the most relationships in the ceRNA network. Furthermore, after hsa_circ_0059655 was knocked down in SACC-83 cells, the expression of hsa-miR-338-3p was up-regulated while CCND1was down-regulated. The proliferation, migration, and invasion of SACC-83 cells also decreased after hsa_circ_0059655 knock-downed.Taken together, the circRNAs derived from PYGB may regulate the tumorigenesis and development of SACC through competing with miR-338-3p.
Abstract Background RNA N6-methyladenine (m6A) modification played an essential role in the occurrence and development of malignant tumors. m6A modification patterns in immune response and tumor microenvironment (TME) remains an enigma. Methods 25 m6A regulators were collected, the molecular alterations and clinical relevance of which were explored. The mutation landscape of the pancreatic adenocarcinoma (PAAD) patients was explored by using TCGA data. The expression difference of the m6A regulators was identified by TCGA and HPA data. The prognosis value of the m6A regulators was measured by TCGA and ICGC data. Consensus clustering analysis was used for different m6A modification patterns identification. CIBERSORT and ESTIMATE algorithms were used to explore the landscape of TME cell infiltration. DEG analysis was used for m6A-related gene identification. m6A-score signature was established by using univariate Cox regression analysis and PCA. Results CNV amplification of m6A regulators led to up-regulated of them in tumor tissues in comparison with normal tissues. 13 of the 25 regulators showed oncogenic features. Two distinct m6A modification patterns were defined. PAAD patients in m6Acluster A occupied better survival compared to m6Acluster B. The relationships between the two m6A patterns and different types of immune infiltrating cells were further identified. A consolidated scoring system to quantify the m6A modification pattern of individual patients was established. Patients in low m6A-score group had better OS compared with these in high m6A-score group. Subsequent analysis proved that m6A methylation modification patterns was associated with response to anti-PD-L1 immunotherapy. Conclusions The molecular alterations and prognostic implications of m6A regulators were analyzed. The distinct m6A modification patterns are crucial for understanding the heterogeneity and complexity of individual tumor microenvironments (TMEs). A comprehensive assessment of m6A modification in tumors enhances our understanding of TME infiltration characteristics and facilitates more effective immunotherapy strategies.
How to obtain a donor liver remains an open issue, especially in the choice of minimally invasive donors right hepatectomy versus open donors right hepatectomy (MIDRH versus ODRH). We conducted a meta-analysis to clarify this question.A meta-analysis was performed in PubMed, Web of Science, EMBASE, Cochrane Central Register, and ClinicalTrials.gov databases. Baseline characteristics and perioperative outcomes were analyzed.A total of 24 retrospective studies were identified. For MIDRH vs. ODRH, the operative time was longer in the MIDRH group (mean difference [MD] = 30.77 min; p = 0.006). MIDRH resulted in significantly less intraoperative blood loss (MD = -57.86 mL; p < 0.00001), shorter length of stay (MD = -1.22 days; p < 0.00001), lower pulmonary (OR = 0.55; p = 0.002) and wound complications (OR = 0.45; p = 0.0007), lower overall complications (OR = 0.79; p = 0.02), and less self-infused morphine consumption (MD = -0.06 days; 95% CI, -1.16 to -0.05; p = 0.03). In the subgroup analysis, similar results were observed in pure laparoscopic donor right hepatectomy (PLDRH) and the propensity score matching group. In addition, there were no significant differences in post-operation liver injury, bile duct complications, Clavien-Dindo ≥ 3 III, readmission, reoperation, and postoperative transfusion between the MIDRH and ODRH groups.We concluded that MIDRH is a safe and feasible alternative to ODRH for living donators, especially in the PLDRH group.
Abstract Chemokine (C-X3-C motif) Receptor 1 (CX3CR1) is a chemokine receptor that functions primarily by mediating the chemotaxis and adhesion of immune cells. However, the role of CX3CR1 in hepatitis C virus (HCV)-induced early-stage liver cirrhosis remains unexplored. GSE15654 retrieved from the GEO. Cox regression model, CIBERSOT and LASSO technique was utilized to identify CX3CR1-associated prognostic genes. Surgical resection samples were collected for verification. High expression of CX3CR1 in the liver was linked to worse prognosis in individuals with HCV-induced early-stage liver cirrhosis. CX3CR1-associated immune infiltration genes(IIGs), namely ACTIN4, CD1E, TMCO1, LOC400499, MTHFD2, and WSF1, were identified, showing specific expression in the livers of individuals with post-hepatic cirrhosis and liver failure compared to HC. Notably, high infiltration of plasma cells and low infiltration of monocytes were predictive of poor prognosis in early-stage cirrhosis. The combined risk model predicted that high expression of CX3CR1-associated IIGs and increased infiltration of plasma cells were associated with unfavorable prognosis in individuals with HCV-induced early-stage liver cirrhosis. Elevated expression of CX3CR1 is a risk factor for individuals with HCV-induced early-stage liver cirrhosis. The developed combined risk model effectively predicted the prognosis of such individuals.
The AHP-LCJ method was used to recognize both the public and the professionals as the main appraisal body.A multi-level appraisal was conducted for the quality of the forest park scenery resources in Wuxie country forest park.The results show that in the whole forest park and scenic appraisal,the public has obtained the high consistency with the professionals.As for scenic spot appraisal,there is a high similarity but with a considerable difference.According to the analysis on the main reason of the difference,forest park's scenery resource appraisal should use the AHP-LCJ and at the same time,consider the public's tourism demand.
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