Strain 12-3T was isolated from seawater of the Guanyinshan Coast, Xiamen, Fujian Province, PR China. The bacterium was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative. Growth of strain 12-3T occurred at 10-37 °C (optimum, 20-30 °C), at pH 5.0-11.0 (optimum, pH 7.0-8.0) and at a salinity range of 0-10 % (optimum, 3-5 %). The results of phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain 12-3T belonged to the genus Paracoccus and had the highest sequence similarity to Paracoccus lutimaris HDM-25T (97.4 %), followed by Paracoccus isoporae SW-3T (96.9 %), Paracoccus caeni MJ17T (96.9 %), Paracoccus pacificus F14T (96.8 %) and other species in the genus Paracoccus (95.3-96.5 %). The average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values between strain 12-3T and P. lutimaris HDM-25T were 76.1 and 17.0 %, respectively. ANI and DDH values between strain 12-3T and P. isoporae SW-3T were 78.9 and 18.2 %, respectively. The principal fatty acid of strain 12-3T was summed feature 8 (C18 : 1 ω6c/ω7c) and C18 : 0. The respiratory quinone of strain 12-3T was Q10. The polar lipids included phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The G+C content of the chromosomal DNA was 63.9 mol%. The combination of the results of the phylogenetic, phenotypic and chemotaxonomic analyses, and its low ANI and DDH values indicate that strain 12-3T represents a novel species of the genus Paracoccus, for which the name Paracoccus xiamenensis sp. nov. is proposed. The type strain is 12-3T (=MCCC 1A16381T=KCTC 72687T).
Objective To investigate the human internal exposure level of several polycyclic aromatic hydrocarbons(PAHs) in the middle school students in a city in south China.Methods The urine samples from 41 middle-schoolboys(16-18 years) of the city in south china were collected in June 2005.After enzymic hydrolysis,the urine samples were enriched and cleaned by solid phase extraction,and then the urinary hydroxy-PAHs were determined by using high performance liquid chromatography with fluorescence detection.Results Seven hydroxy polycyclic aromatic hydrocarbons were detected in the urinary samples.The range,median and mean concentrations of these metabolites were : 2-hydroxy naphthalene(0.58-14.95,3.62,4.27 μmol/mol creatinine),2-hydroxy fluorene(0.68-6.17,1.55,1.74 μmol/mol creatinine),2-hydrol phenanthrene+3-hydroxyl phenanthrene(0.09-0.69,0.23,0.25 μmol/mol creatinine),9-hydroxy phenanthrene(0.19-4.94,0.45,0.72 μmol/mol creatinine),4-hydroxy phenanthrene(LOD-0.13,LOD,0.02 μmol/mol creatinine) and 1-hydroxy pyrene(0.06-0.58,0.18,0.22 μmol/mol creatinine).Conclusion The median concentration of 1-hydroxy pyrene in this study was a slightly higher than those of the background population in northeast China(0.13 μmol/mol creatinine) and Beijing(0.15 μmol/mol creatinine).However,it is obviously higher than the reported data from international middle school students in Europe,American and Keroean(0.08 μmol/mol creatinine).The results of spearman’s correlation matrix between different metabolites indicate that multi-determination of hydroxy-PAH will be helpful to assess the PAH exposure level of human.
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