Christos Liaskos

University of Thessaly

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Dimitrios P. Bogdanos

University Hospital of Larissa

Eirini I. Rigopoulou

University Hospital of Larissa

Lazaros I. Sakkas

University of Thessaly

Theodora Simopoulou

University Hospital of Larissa

Wolfgang Meyer

Euroimmun Medizinische Labordiagnostika (Germany)

Thomas Scheper

Euroimmun Medizinische Labordiagnostika (Germany)

ΑM

Αthanasios Mavropoulos

University of Thessaly

Christina Katsiari

University Hospital of Larissa

GΝ

George Ν. Dalekos

University of Thessaly

Kalliopi Zachou

University Hospital of Larissa

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University of Thessaly

105

University Hospital of Larissa

Aristotle University of Thessaloniki

National and Kapodistrian University of Athens

King's College Hospital

King's College London

University of Ioannina

Center for Research and Technology Thessaly

Euroimmun Medizinische Labordiagnostika (Germany)

Center for Rheumatology

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Human cytomegalovirus (HCMV) UL44 and UL57 specific antibody responses in anti-HCMV-positive patients with systemic sclerosis

Clinical Rheumatology (2017)

Εμμανουέλα Μάρου Christos Liaskos Theodora Simopoulou Georgios Efthymiou Efthimios Dardiotis

Scleroderma (fungus)

Cytomegalovirus

10.1007/s10067-017-3553-5

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Citations (14)

HCV-RNA qualitative assay based on transcription mediated amplification improves the detection of hepatitis C virus infection in patients on hemodialysis: Results from five hemodialysis units in central Greece

Journal of Clinical Virology (2005)

Eirini I. Rigopoulou Ioannis Stefanidis Christos Liaskos Eleftheria Zervou Christos V. Rizos

Hepatitis C

10.1016/j.jcv.2005.05.007

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Citations (51)

Apremilast increases IL-10-producing regulatory B cells and decreases proinflammatory T cells and innate cells in psoriatic arthritis and psoriasis

Lara D. Veeken (2019)

Αthanasios Mavropoulos Efterpi Zafiriou Theodora Simopoulou Alexandros G. Brotis Christos Liaskos

Abstract Objectives Psoriatic arthritis (PsA) and psoriasis are immune-mediated inflammatory diseases sharing common immunological mechanisms. Regulatory B cells (Breg cells) producing IL–10 (B10 cells), a critical anti-inflammatory B-cell subset, were found to be decreased in both PsA and psoriasis. Apremilast, a phosphodiesterase-4(PDE4) inhibitor, increases IL-10 and therefore, we examined the effect of apremilast on Breg cells. Methods Fifty patients, including 20 with PsA and 30 with psoriasis, were included in the study. The effect of apremilast on Breg cells at 3, 6 and 12 months post-treatment, was examined by flow cytometry in ODN2006 (TLR9)-stimulated peripheral blood mononuclear cells and magnetically-isolated cells. Th1 cells, Th17 cells and NKT were also measured. Results Ex vivo stimulated cell analysis identified that post-apremilast (IL-10+CD19+) B10 cells were increased in all PsA and psoriasis patients and correlated with psoriatic skin and joint clinical improvement. Apremilast decreased IFNγ(+) T and NKT cells and IL-17(+)NKT cells. B10 cells also inversely correlated with Th1 cells, and IFNγ(+)NKT cells. Conclusion These results suggest that Breg cells are a major target of apremilast in PsA and psoriasis and that apremilast-induced increase of Breg cells is associated with a decrease of Th1 cells, IFNγ-producing NKT cells and IL-17-producing NKT cells.

Apremilast

Regulatory B cells

10.1093/rheumatology/kez204

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Citations (40)

Anti‐C1q antibodies in scleroderma

British Journal of Dermatology (2019)

Christos Liaskos Stamatina Rentouli Theodora Simopoulou Athanasios Gkoutzourelas G.L. Norman

Systemic sclerosis (SSc) is an autoimmune condition which means that the body's immune system, which usually fights off disease, wrongly targets healthy parts of the body, and SSc patients produce antibodies to their own tissues (‘autoantibodies’). Extensive thickening and scarring of tissues (fibrosis) is the main feature of the condition and is due to over‐production of collagen. Changes in small blood vessels also occur. When fibrosis occurs in the lungs (pulmonary fibrosis) it can lead to high blood pressure in the arteries going to the lungs, and this may be life‐threatening. The condition is commoner in women. SSc can be localised or disseminated (generalised), depending on the extent of skin involvement. Although the cause of SSc has not been fully worked out, many of the autoantibodies associated with the condition are believed to be involved in one way or another. Autoantibodies called ‘anti‐C1q’ are certainly found in another autoimmune condition called systemic lupus erythematosus, so the aim of this study from Greece and the USA was to see if they were also present in SSc. Anti‐C1q autoantibodies were found in 16.1% of the 124 SSc patients in the study, occurring as often as the third most frequently found autoantibody, known as anti‐RNA polymerase III. They were found particularly in men and in patients with disseminated SSc. Presence of anti‐C1q antibodies was associated with pulmonary fibrosis but not with any of the other features of SSc. The authors therefore suggest that anti‐C1q autoantibodies could be looked at as a marker for (sign of) pulmonary fibrosis in SSc patients.

Scleroderma (fungus)

10.1111/bjd.18066

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526 Occult hepatitis B virus infection in Greek patients with chronic hepatitis C and in patients with diverse non-viral hepatic diseases

Journal of Hepatology (2004)

S. P. Georgiadou Kalliopi Zachou Eirini I. Rigopoulou Christos Liaskos Paraskevi Mina

Occult

Viral Hepatitis

10.1016/s0168-8278(04)90526-0

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Citations (2)

Bone marrow findings in patients with autoimmune liver diseases

Journal of Gastroenterology and Hepatology (2008)

Aikaterini Tsikrikoni Eirini I. Rigopoulou Kalliopi Zachou Christos Liaskos Despina Kyriakou

Abstract Background and Aims: We have recently reported quantitative and qualitative differences of the bone marrow (BM) hematopoietic progenitor cells in autoimmune hepatitis type‐1 (AIH‐1) and primary biliary cirrhosis (PBC). In order to better investigate the possible involvement of BM in these diseases, we studied the morphological abnormalities of BM aspirates in the same patients with AIH‐1 and PBC. Methods: BM smears from 13 AIH‐1 and 13 PBC patients were investigated. BM from 12 patients with cirrhosis of non‐autoimmune etiology and eight healthy individuals served as pathological (PC) and healthy controls (HC). Results: Erythroid, granulocyte and platelet precursors were variably altered. Polychromatic normoblasts and immature megakayocytes were higher in AIH‐1 (11.9 ± 2.9 and 16.2 ± 16.9, respectively) and PBC (10.2 ± 3.6 and 17.3 ± 20.2, respectively) compared to PC (7 ± 2 and 2.3 ± 6.0, respectively) and HC (7.9 ± 1.6 and 0 ± 0, respectively) ( P = 0.0001 and P = 0.006). In AIH‐1, immature megakaryocytes were significantly higher in patients receiving immunosuppression (25.71 ± 17.66 vs 5.00 ± 5.48; P < 0.02) and were associated negatively with laboratory markers of disease activity. BM plasmacytosis was observed more frequently in AIH‐1 compared to PBC and PC. BM monocytosis was found in all patients with AIH‐1, PBC and PC whereas approximately half of the patients with autoimmune liver diseases and all PC had BM lymphocytosis. Conclusions: BM monocytosis and lymphocytosis are commonly found in AIH‐1 and PBC patients irrespective of the presence of cirrhosis or the use of immunosuppression. BM plasmacytosis appears to be a distinct finding in some AIH‐1 patients, as similar findings were observed in only one PBC and one PC, whereas BM immature megakaryocytes characterize both AIH‐1 and PBC. Whether BM is a target organ in AIH‐1 and PBC needs further investigation.

Monocytosis

Autoimmune Hepatitis

Lymphocytosis

Primary Biliary Cirrhosis

10.1111/j.1440-1746.2008.05309.x

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Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

Journal of Immunology Research (2014)

Αthanasios Mavropoulos Dimitrios P. Bogdanos Christos Liaskos Timoklia Orfanidou Theodora Simopoulou

Flow cytometric analysis of p38 mitogen-activated protein kinase (p38 MAPK) signaling cascade is optimally achieved by methanol permeabilization protocols. Such protocols suffer from the difficulties to accurately detect intracellular cytokines and surface epitopes of infrequent cell subpopulations, which are removed by methanol. To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK. These included markers of B cells (CD19, CD20, and CD22), T cells (CD3, CD4, and CD8), NK (CD56 and CD7), and dendritic cells (CD11c). We have also tested surface markers of costimulatory molecules, such as CD27. We have successfully determined simultaneous expression of IFN- γ , as well as IL-10, and phosphorylated p38 in cell subsets. The optimized phosphoflow protocol has also been successfully applied in peripheral blood mononuclear cells or purified cell subpopulations from patients with various autoimmune diseases. In conclusion, our refined phosphoflow cytometric approach allows simultaneous detection of p38 MAPK activity and intracellular cytokine expression and could be used as an important tool to study signaling cascades in autoimmunity.

CD11c

10.1155/2014/671431

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Citations (8)

Antimitochondrial antibodies of immunoglobulin G3 subclass are associated with a more severe disease course in primary biliary cirrhosis

Liver International (2007)

Eirini I. Rigopoulou Edward T. Davies Dimitrios P. Bogdanos Christos Liaskos Maria Mytilinaiou

Abstract Background/Aims: Primary biliary cirrhosis (PBC) is characterised by the presence of immunoglobulin (Ig) G antimitochondrial antibodies (AMA), which are routinely detected by indirect immunofluorescence (IFL) using composite rodent tissue substrate. The IgG subclass distribution and clinical significance of IFL‐detected AMA in patients with PBC have not been previously studied in detail. Methods: We have examined IgG subclass‐specific AMA detected by IFL on rodent liver, kidney and stomach tissue substrate using affinity‐purified IgG subclass monospecific antisera as revealing reagents in 95 AMA‐positive PBC patients from Greece. Results: AMA of any of the IgG1, IgG2 or IgG3 subclasses were present in 89/95 (93.7%) patients. Among those 89, 55 (61.8%) had IgG1, 2, 3 AMA positivity; eight (9%) had IgG1, 2; seven (7.9%) had IgG2, 3; eight (9%) had IgG1, 3; nine (10.1%) had IgG1 subclass and two (2.2%) single IgG3 AMA reactivity. IgG4 AMA was absent. IgG3 titres were higher than IgG2 and IgG1 ( P <0.001) and IgG1 higher than IgG2 ( P <0.001). IgG3 AMA‐positive patients had a histologically more advanced disease ( P <0.01) and were more frequently cirrhotic compared with those who were negative ( P <0.01). There was a positive correlation between AMA IgG3 titre and Mayo risk score ( r =0.55, P =0.009, Spearman's correlation). Conclusions: Our findings suggest that AMA are not restricted to a specific IgG subclass. AMA of the IgG3 subclass are associated with a more severe disease course, possibly reflecting the peculiar ability of this isotype to engage mediators of damage.

Subclass

Primary Biliary Cirrhosis

10.1111/j.1478-3231.2007.01586.x

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Citations (63)

Diagnostic relevance of anti-filamentous actin antibodies in autoimmune hepatitis: Figure 1

Journal of Clinical Pathology (2007)

Christos Liaskos Dimitrios P. Bogdanos Edward T. Davies George Ν. Dalekos

We read with interest the paper by Granito et al 1 reporting on the clinical and diagnostic significance of anti-filamentous actin antibodies (A-FAA) in autoimmune hepatitis type 1 (AIH-1). The authors found that A-FAA, measured by a new commercially available ELISA based on a modified cut-off of 30 instead of the manufacturer’s 20 arbitary units (AU), strictly correlates with the smooth muscle antibody glomerular/tubular (SMA-G/T) pattern,2 also known as the microfilament pattern, mostly seen in patients with AIH-1.1 Their findings further indicate F-actin as the predominant, if not the sole, target of AIH-1-specific SMA reactivity, a notion that has been questioned in the past because of the inconsistent results obtained by several actin-based assays.3,4 We agree with Granito et al 1 …

Autoimmune Hepatitis

Clinical Significance

10.1136/jcp.2006.039404

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Citations (27)

Investigating the link between Helicobacter pylori infection and psoriatic disease: an immunological study

Immunologic Research (2024)

Eleni Patrikiou Georgios Efthymiou Christos Liaskos Niki Ntavari Thomas Scheper

Flagellin

Helicobacter

10.1007/s12026-024-09569-3

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Citations (0)