Methods Fibroblasts primary culture was established from 11 breast cancer patients. Expression analysis was evaluated in PT (n=4), N+ (n=3) and BM (n=4) through a customized cDNA microarray platform (4,800 ORESTES) analyzed by SAM (TMEV; FDR 0%) and functional analysis was performed using DAVID. Technical validation was performed in 6 samples and biological validation was performed in fibroblasts obtained from others 25 patients as evaluated by RT-qPCR.
The epithelioid hemangioma (EH), also known as angiolymphoid hyperplasia with eosinophilia, is a benign, neoplastic vascular tumor rarely presented in the mouth. It is most frequent in young adult male patients. Clinically, it is characterized by papules or nodules, usually with a reddish-brown color, mimicking other vascular and non-vascular lesions. Histologically, the lesion is composed of well-formed round vessels with epithelioid endothelial cells, with a histiocytoid appearance, surrounded by an infiltrate of lymphocytes and eosinophils. Oral EH’s endothelial cells are commonly immunoreactive for CD34 and factor VIII antigen. Surgical excision is the most widely used form of treatment, and recurrences are uncommon. The aim of this study was to report a rare case of oral EH in a 52-year-old female patient.
14612 Background: Breast cancer patients present lower 1,25(OH)2D3 or 25(OH)D3 serum levels than unaffected women. Although breast cancer cell lines are growth inhibited by to vitamin D supra-physiological concentrations, there is much uncertainty about the anti- proliferative effect of physiological concentrations of 1,25(OH)2D3, the active form of vitamin D, in human beings. Vitamin D supplementation to post-menopausal women may be indicated to reduce bone loss. Methods: We have evaluated tumor dimension, proliferation rate (Ki67 expression), 1,25(OH)2D3 serum concentration, before and after a one month calcitriol supplementation (dose to prevent osteoporosis) to post-menopausal patients. Twenty one patients with clinical stage II disease, signed the informed consent and took part in the study from Jan- Nov 2007. Median age was 56 years (50–66), all tumors were grade 2/3, ER was detected in 11/18 and HER2 in 8/18 tumors; two tumors were triple negative. The first 10 (group-GI) and the next 11 patients (group-GII) received calcitriol 0.25 and 0.50mg/day PO, respectively. Results: One third of the patients presented 25(OH)D3 insufficiency (7–20 ng/mL) and no patients presented 1,25(OH)2D3 serum concentration below the reference level (15.9 pg/mL). No differences in 1,25(OH)2D3 serum concentration (GI: 63.05±20.44 pg/mL vs 56.20±25.16, p=0.16; GII: 69.77±39.02 vs 49.33±21.01, p=0.22, paired t test) and tumor dimension evaluated by ultrasonography (GI: 27.67 ±.9.92 mm vs 26.70±8.15, p=0.57; GII: 30.82±8.21 vs 30.00±11.96, p=0,76) were detected before and after calcitriol supplementation, in both groups. There was a trend towards a reduction in Ki67 expression in GI (10.0±6.6% vs 4.5±4.7, p=0.06, paired t test) and a significant reduction in GII (9.13±1.27% vs 4.59±1.42, p=0.02). Two patients with triple negative tumors had major reductions of Ki67 expression following calcitriol supplementation (6.6–11 to 0.6–2%). Conclusions: In a small group of post-menopausal patients, a short period of calcitriol supplementation may reduce Ki67 expression in breast cancer specimens, suggesting that physiological doses of the active form of vitamin D may have antiproliferative effects in these patients. Supported by FAPESP No significant financial relationships to disclose.
Cementoblastoma is a rare benign lesion that represents less than 1% of all odontogenic tumours. It's characterized by proliferation of cementum-like tissue and in almost all cases tends to be associated with an erupted permanent tooth, most often the first mo- lar. We present an unusual case of a large cemento-blastoma that affected the right mandibular body, extending from the first premolar to the second molar, of a 19-years-old male. In this case an initial surgery was attempted under local anaesthesia, resulting in incomplete tumor removal. A second surgical procedure was performed under general anaesthesia, ensuring the complete excision of the lesion. The patient was monitored for 1 year after surgery and did not show any signs of recurrence.
A Chronic Sclerosing Sialodenitis is a rare and benign lesion that preferentially affects a submandibular gland of male patients. Although its etiology is considered unknown, it is suggested that the secretion of IgG4 immunoglobulin may be useful for its manifestation. This pathology has specific clinical characteristics for malignant neoplasms of the salivary glands, where the recommended treatment is a surgical excision. The present study presents an atypical case of chronic sclerosing sialodenitis that involves a woman's soft palate, where there was a complete resolution of the clinical case, in which the conservative treatment was chosen by the use of medications.