Xiuqin Zhao

National Institute for Communicable Disease Control and Prevention

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Kanglin Wan

Nantong University

Haican Liu

National Institute for Communicable Disease Control and Prevention

Guilian Li

National Institute for Communicable Disease Control and Prevention

Yi Jiang

Huazhong Agricultural University

Zhiguang Liu

Northwest A&F University

Lili Zhao

China Agricultural University

Machao Li

National Institute for Communicable Disease Control and Prevention

Haiyan Dong

University of Science and Technology of China

Wan Kang-lin

National Institute for Communicable Disease Control and Prevention

Tongyang Xiao

Sun Yat-sen University

Cooperative Institutions

National Institute for Communicable Disease Control and Prevention

Chinese Academy of Medical Sciences & Peking Union Medical College

Capital Medical University

Chinese Academy of Sciences

Peking University

Sun Yat-sen University

Shanghai Jiao Tong University

Fudan University

Huazhong University of Science and Technology

Chinese Center For Disease Control and Prevention

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Molecular Typing Characteristic and Drug Susceptibility Analysis ofMycobacterium tuberculosisIsolates from Zigong, China

BioMed Research International (2016)

Haican Liu Jianping Deng Haiyan Dong Ti-Quan Xiao Xiuqin Zhao

China is one of the 22 countries with high TB burden worldwide, and Sichuan contained the second-largest number of TB cases among all of the Chinese provinces. But the characteristics of Mycobacterium tuberculosis circulated in Zigong, Sichuan, were still unknown. To investigate the character and drug resistance profile, 265 clinical isolates were cultured from tuberculosis patient's sputum samples in the year of 2010, of which the genetic profile was determined by using Spoligotyping and MIRU-VNTR typing methods, and the drug sensibility testing to the four first-line and four second-line antituberculosis (anti-TB) drugs was performed by using proportion method on Lowenstein-Jensen (L-J) media. The major Spoligotype was Beijing family (143/265, 53.96%), followed by T (80/265, 30.19%) and H (9/265, 3.40%) genotypes; the total Hunter-Gaston discrimination index (HGDI) of the 24 loci MIRU-VNTR was 0.9995. About 27.17% (72/265) of the isolates were resistant to at least one of the eight tested anti-TB drugs, and for Beijing and non-Beijing family isolates the proportion of drug resistance was 28.47% (41/144) and 25.62% (31/121), respectively. That is, the most prevalent genotype here was Beijing family, and the 24 loci VNTR analysis could supply a high resolution for genotyping, and Beijing and non-Beijing isolates had no difference (p > 0.05) for drug resistance.

10.1155/2016/6790985

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Citations (15)

Ocular Comorbidities among Cataract-Operated Patients in Rural China

Ophthalmology (2007)

Yingpeng Liu Nathan Congdon Hoi Fan Xiuqin Zhao Kai Chow Choi

Visual Impairment

10.1016/j.ophtha.2007.07.013

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Citations (17)

Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct annotated encoding genes

Genomics (2021)

Jiahui Shi Shuhong Meng Li Wan Zhenpeng Zhang Songhao Jiang

Mycobacterium tuberculosis (MTB) is a severe causing agent of tuberculosis (TB). Although H37Rv, the type strain of M. tuberculosis was sequenced in 1998, annotation errors of encoding genes have been frequently reported in hundreds of papers. This phenomenon is particularly severe at the 5′ end of the genes. Here, we applied a TMPP [(N-Succinimidyloxycarbonylmethyl) tris (2,4,6-trimethoxyphenyl) phosphonium bromide] labeling combined with StageTip separating strategy on M. tuberculosis H37Rv to characterize the N-terminal start sites of its annotated encoding genes. Totally, 1047 proteins were identified with 2058 TMPP labeled N-terminal peptides from all the 2625 mass spectrometer (MS) sequenced proteins. Comparative genomics analysis allowed the re-annotation of 43 proteins' N-termini in H37Rv and 762 proteins in Mycobacteriaceae. All revised N-termini start sites were distributed in 5'-UTR of annotated genes due to over-annotation of previous N-terminal initiation codon, especially the ATG. In addition, we identified and verified a novel gene Rv1078A in +3 frame different from the annotated gene Rv1078 in +2 frame. Altogether, our findings contribute to the better understanding of N-terminal of H37Rv and other species from Mycobacteriaceae that can assist future studies on biological study.

Gene Annotation

10.1016/j.ygeno.2021.12.001

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Epidemiological characteristics and trends of notifiable infectious diseases in China from 1986 to 2016

Journal of Global Health (2020)

Yi Jiang Xiangfeng Dou Chenqi Yan Li Wan Haican Liu

Since the 1980s, China has undergone significant social change and the incidence of infectious diseases has also changed considerably. Here, we report the epidemiological features and changes in notifiable infectious diseases in China from 1986 to 2016 to explore the factors contributing to the successful control of infectious diseases and the challenges faced in the prevention and control of infectious diseases.The data of notifiable infectious diseases in China from 1986 to 2016 were collected from the monthly analysis report of the National Infectious Disease Surveillance System. Joinpoint regression models were used to examine incidence and mortality trends from 1986 to 2016. IBM SPSS Statistics version 22.0, Excel 2010 and R x64 3.5.2 were used for data analysis.A total of 132 858 005 cases of notifiable infectious diseases were reported over these 31 years, with an average yearly incidence of 342.14/100 000. There were 284 694 deaths with an average yearly mortality rate of 0.73/100 000. The overall incidence and overall mortality of notifiable infectious diseases both showed a "U" distribution (ie, a decrease, stable, an increase, stable again). The top five diseases in terms of incidence were hand, foot and mouth disease, viral hepatitis, tuberculosis, other infectious causes of diarrhea and dysentery, accounting for 78.0% of all reported cases. The top five causes of death were HIV/AIDS, rabies, tuberculosis, viral hepatitis and epidemic encephalitis B, which accounted for 76.07% of all mortalities. The diseases with the top five fatality rates were rabies, H5N1, H7N9, HIV/AIDS and plague, with rates of 91.06%, 66.07%, 38.51%, 25.19% and 10.31%, respectively.This analysis will benefit the future monitoring of infectious diseases and public health measures in China.

Case fatality rate

Viral Hepatitis

Notifiable disease

10.7189/jogh.10.020803

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Citations (41)

Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

Antimicrobial Agents and Chemotherapy (2014)

Lili Zhao Yan Chen Haican Liu Qiang Xia Xiaocui Wu

ABSTRACT To investigate the molecular characterization of multidrug-resistant tuberculosis (MDR-TB) isolates from China and the association of specific mutations conferring drug resistance with strains of different genotypes, we performed spoligotyping and sequenced nine loci ( katG , inhA , the oxyR-ahpC intergenic region, rpoB , tlyA , eis , rrs , gyrA , and gyrB ) for 128 MDR-TB isolates. Our results showed that 108 isolates (84.4%) were Beijing family strains, 64 (59.3%) of which were identified as modern Beijing strains. Compared with the phenotypic data, the sensitivity and specificity of DNA sequencing were 89.1% and 100.0%, respectively, for isoniazid (INH) resistance, 93.8% and 100.0% for rifampin (RIF) resistance, 60.0% and 99.4% for capreomycin (CAP) resistance, 84.6% and 99.4% for kanamycin (KAN) resistance, and 90.0% and 100.0% for ofloxacin (OFX) resistance. The most prevalent mutations among the MDR-TB isolates were katG315 , inhA15 , rpoB531 , - 526 , and - 516 , rrs1401 , eis-10 , and gyrA94 , - 90 , and - 91 . Furthermore, there was no association between specific resistance-conferring mutations and the strain genotype. These findings will be helpful for the establishment of rapid molecular diagnostic methods to be implemented in China.

INHA

rpoB

Capreomycin

Kanamycin

Intergenic region

10.1128/aac.01792-13

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Citations (31)

[Comparison on discriminatory power of different variable number tandem repeats locus-set on genotyping of mycobacterium tuberculosis isolated in China].

PubMed (2009)

Zhaona Li Mei Liu Bing Lü Xiuqin Zhao Zhiguang Liu

To evaluate the application of different variable number tandem repeats (VNTR) locus in genotyping of Mycobacterium tuberculosis (M.tuberculosis) strains isolated from eight provinces in China, and to find the suitable locus-set of VNTR for epidemical strains in China.All 140 M.tuberculosis strains were randomly selected from 2800 M.tuberculosis strains isolated from eight provinces in China, 27 VNTR loci were used for typing all isolates. Discriminatory power (Hunter-Gaston Index, HGI) of every locus and different locus-set were analyzed by BioNumerics software. Meanwhile, Spoligotyping was used to identify Beijing family and non-Beijing family. Then the HGI of different locus-sets in two families was also evaluated.All 140 isolates were clustered into Beijing kindred (112 strains, 80%) and non-Beijing kindred (28 strains, 20%) by Spoligotyping. The discriminatory power of Spoligotyping in 140 isolates was 0.4589. Every locus showed different polymorphism and HGI were from 0 to 0.809. The number of VNTR loci with HGI higher than 0.5 in all strains, Beijing family and non-Beijing family was 8, 7 and 14 respectively. 27 loci were combined into four groups which included 8, 12, 15 and 24 VNTR loci respectively. Four locus-sets showed different polymorphism, HGI of eight-locus, 12-locus, 15-locus, and 24-locus set in 140 strains was 0.9991, 0.9882, 0.9980 and 0.9986, and their discriminatory power were calculated in Beijing kindred (HGI: 0.9987, 0.9318, 0.9969 and 0.9975) and non-Beijing kindred (HGI: 1, 0.9894, 1 and 1).Different VNTR locus and locus-set showed different discriminatory power in the selected M.tuberculosis strains isolated from China. Eight-locus set can be used in molecular epidemiological study of M.tuberculosis in China after standardization.

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A real-time PCR assay based on a specific mutation of PstS1 gene for detection of M. bovis strains

Biologicals (2020)

Lingyun Ji Yi Jiang Guilian Li Xiuqin Zhao Kanglin Wan

Mycobacterium tuberculosis complex

Strain (injury)

10.1016/j.biologicals.2020.01.007

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Citations (4)

Spillover effects of Japanese monetary policy on China: Is there any differences during and aftermath of crisis?

Xiuqin Zhao Zhanqiong He

This paper examines the spillover effects of Japanese monetary policy on Chinese macro economy - during and in the aftermath of 2007–2008 financial crisis. Study periods are divided into two: from January 2007 to January 2013 as the first period and from April 2013 to December 2015 as the second one. The reason why we take 2013 as threshold is that since 2013, Japan executed more radical quantitative easing policy and China experienced an obvious slow economic growth and deepen market oriented interest rate reform and exchange rate reform. The results of VAR model analysis shows that Japanese monetary policy did have spillover effects on China economy, while behavior of two stages were different. Japanese monetary policy mainly affected Chinese money supply, exchange rates and interest rates at the first stage, while it mainly affected Chinese output and trade level at the second stage.

Spillover effect

Chinese economy

10.1109/icsssm.2016.7538596

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[Study on the characteristics of mutation on Mycobacterium tuberculosis rifampicin-resistance gene].

PubMed (2006)

Jinghua Liu Li-shui Zhang Zhiguang Liu Mingxiang Huang Xiuqin Zhao

To elucidate the characters of rpoB mutation in rifampin-resistant clinical isolates of Mycobacterium tuberculosis.286 bp DNA fragment of rpoB gene including 81 bp code region (rifampin resistance deteremination region, RRDR) was analyzed by PCR-single-strand conformation polymorphism(SSCP). The 286 bp DNA fragment of each strain which had been proved to have mutation by PCR-SSCP was then sequenced. 110 strains of M. tuberculosis, including 73 rifampin-resistant strains, 11 rifampin-susceptible drug-resistant strains and 26 drug-susceptible strains were studied.47 rifampin-resistant strains were detected to have mutations by PCR-SSCP method. 76.6% rifampin-resistant strains showed that rpoB gene was carrying single point mutation analyzed by direct sequencing technique, which mainly located at 531-Ser (61.1%) and 526-His (25.0%). The combined mutation rate was 23.4%. In addition, 2 rifampin-susceptible drug-resistant strains and 1 drug-susceptible strain were mutated, detected by PCR-SSCP method. Sequencing results showed that the mutations located at 511-Leu, 526-His and 535-Pro.Mutations in the 81 bp RRDR of rpoB were the main reasons of M. tuberculosis resistant to rifampin. 531-Ser and 526-His were the most common positions of mutations.

rpoB

Single-strand conformation polymorphism

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[Polymorphisms of toxin-antitoxin-chaperone system of Mycobacterium tuberculosis complex in China].

PubMed (2016)

Tongyang Xiao Lili Zhao Haican Liu Machao Li Xiuqin Zhao

To investigate the single nucleotide polymorphism (SNP) of toxin-antitoxin-chaperone (TAC) system of Mycobacterium (M.) tuberculosis with different genotypes and its biological significance.A total of 183 clinical M. tuberculosis isolates were collected for spoligotyping. The sequences of higA,higB and Rv1957 were obtained by using PCR and DNA sequencing. The sequences were compared for possible mutations. Functional consequences of nonsynonymous SNPs were predicted by using I-Mutant 2.0 servers.Among the 183 M. tuberculosis isolates, 138(75.41%) belonged to the Beijing family, while 45(24.59%) belonged to the non-Beijing family. A total of 149(81.42%) isolates showed polymorphisms in the TAC system. We discovered 6 nonsynonymous SNPs and 2 synonymous SNPs. All the synonymous mutations occurred in higA gene, while nonsynonymous SNPs were found in the higA,higB and Rv1957 genes either. All the synonymous mutations and 4 nonsynonymous SNPs were restricted to the Beijing family strains and only 2 nonsynonymous SNPs were observed in the non-Beijing family strains. Of the 6 nonsynonymous SNPs studied, 4 were predicted to have ability to affect the stability of respective protein.The SNPs in the coding sequences of TAC system in clinical isolates can be relatively high and the Beijing family strains are with higher polymorphism, which might benefit to adapt to different host environment.

Nonsynonymous substitution

SNP genotyping

Synonymous substitution

10.3760/cma.j.issn.0254-6450.2016.03.021

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Citations (1)