This study aimed to investigate the effect of isoproterenol pre-treatment on the therapeutic efficacy of cardiosphere-derived cells (CDCs) transplantation for myocardial infarction (MI).Thirty 8-week-old male Sprague-Dawley (SD) rat model of MI was generated by ligation of the left anterior descending artery. The MI rats were treated with PBS (MI group, n=8), CDCs (MI + CDC group, n=8) and isoproterenol pre-treated CDCs (MI + ISO-CDC group, n=8), respectively. In the MI + ISO-CDC group, CDCs were pre-treated by 10-6 M isoproterenol and the cultured for additional 72 h, then injected to the myocardial infraction area like other groups. At 3 weeks after the operation, echocardiographic, hemodynamic, histological assessments and Western blot were performed to compare the CDCs differentiation degree and therapeutic effect.Isoproterenol treatment (10-6 M) simultaneously inhibited proliferation and induced apoptosis of CDCs, up-regulated proteins of vimentin, cTnT, α-sarcomeric actin and connexin 43, and down-regulated c-Kit proteins (all P<0.05). The echocardiographic and hemodynamic analysis demonstrated that the MI rats in the two CDCs transplantation groups had significantly better recovery of cardiac function than the MI group (all P<0.05). MI + ISO-CDC group had better recovery of cardiac function than the MI + CDC group, although the differences did not reach significant. Immunofluorescence staining showed that the MI + ISO-CDC group had more EdU-positive (proliferating) cells and cardiomyocytes in the infarct area than the MI + CDC group. MI + ISO-CDC group had significantly higher protein levels of c-Kit, CD31, cTnT, α-sarcomeric actin and α-SMA in the infarct area than the MI + CDC group.These results suggested that in CDCs transplantation, isoproterenol pre-treated CDCs can provide a better protective effect against MI than the untreated CDCs.
The high defect density on the surface of AlGaN material with high Al composition limits its application in the field of solar-blind ultraviolet (UV) detection. In this work, a surface modification method for AlGaN has been proposed to enhance the performance of AlGaN metal–semiconductor–metal (MSM) solar-blind UV photodetectors (PDs). The 5,10,15,20-T(4-OH P)P (C 44 H 30 N 4 O 4 ) organic molecules are chemically adsorbed on the surface of high-Al-content Al 0.6 Ga 0.4 N MSM PD, which can reduce the interface potential caused by material defects and increase the photogenerated carriers of the device. Compared with the control unmodified PD, the C 44 H 30 N 4 O 4 modification method significantly increases the photocurrent and responsivity of the PD, which is beneficial to improve the optoelectronic performances of III-nitride-based PDs.
Abstract ADP-ribosylation is the enzymatic transfer of the ADP-ribose moiety from NAD+ to a protein, a posttranslational modification that regulates the function of the targeted proteins. Although in normal conditions there is minimal extracellular NAD+, it is released from cells in inflammation and during preparation of primary lymphocytes. ART2 (CD296) is an ADP-ribosyltransferase expressed by most mature T cells, including NKT, and regulatory T cells. A major substrate for ART2 is P2X7, an ion channel that mediates shedding of CD62L, and NAD+-induced cell death (NICD). In this manner, NAD+ released during cell preparation affects the phenotype and survival of cells expressing ART2. In this study, we used a recombinant variable domain of the llama heavy-chain antibody S+16a to block the ADP-ribosyltransferase activity of ART2. The recombinant antibody was administered i.v. to mice and 1-2 h later, the CD4+ T cells from spleen and lymph nodes were isolated. The cell phenotype and functional state was analyzed by flow cytometry and chemotaxis assay respectively. A single dose of S+16a blocked the shedding of CD62L and reduced the number of apoptotic cells about 80%. The higher cell viability resulted in a 50-100% increase in the cell number responding to the chemokines CCL22 and CXCL12. We conclude that S+16a is a useful tool to protect mature T cells from NICD induced by the released NAD+ from cells during purification, thereby enhancing the efficiency of in vitro migration assays.
Correction for ‘Largely enhanced thermoelectric effect and pure spin current in silicene-based devices under hydrogen modification’ by G. Qiao et al., Nanoscale, 2020, 12, 277–288, DOI: 10.1039/C9NR07541K.
Abstract Cellular therapies such as chimeric antigen receptor (CAR) T or NK cells undergo phenotypic characterization during discovery and development of novel therapies, biomanufacturing, and upstream patient cell processing for clinical use. Samples are routinely analyzed via flow cytometry for T cell, NK cell, B cell, and monocyte surface marker expression. Image cytometry systems perform cell-based assays that can be used as a convenient alternative to flow cytometry. Recently, a new high-throughput image cytometer, the Cellaca PLX system (Nexcelom, Lawrence, MA) was developed for immunophenotyping, transfection/transduction efficiency, and cell health assays. This new instrument can assess several critical quality attributes (CQAs) such as cell identity, viability, and other relevant biological functions recommended by the International Organization for Standardization using the ISO Cell Characterization that focuses on cellular therapeutic products. Here we demonstrate a rapid and high-throughput immunophenotyping and viability detection in peripheral blood mononuclear cells (PBMCs) using the Cellaca PLX. PBMCs underwent red blood cell (RBC) lysis and CD3 enrichment, were stained with Hoechst/CD3/CD4/CD8 or Hoechst/CD3/CD8/RubyDead surface marker kits and measured on the Cellaca PLX and three different flow cytometers for comparison. Results showed highly comparable cell populations between the flow cytometers and the Cellaca PLX suggesting that the image cytometer may provide a rapid and convenient alternative method for immunophenotyping. The proposed method can streamline workflows from sample staining to data analysis, quickly moving precious patient samples into downstream processes.
Abstract Traditional fluorescent anti‐counterfeiting labels can be easily cloned due to the lack of sufficient security. Therefore, multilevel anti‐counterfeiting techniques with higher security need to be constantly explored. This study reports a multilayer composite structure containing Mn doped CsPbCl 3 (Mn:CsPbCl 3 ) perovskite quantum dots (PQDs) layer and CsPbBr 3 PQDs layer. The encrypted information is patterned on the Mn:CsPbCl 3 PQDs layer with weak orange fluorescence, which is concealed by the brighter green fluorescence of CsPbBr 3 PQDs at the upper layer. Based on the well‐separated photoluminescence (PL) peak positions, inverse PL temperature‐dependences and distinct PL lifetimes of these two PQDs, the encrypted information can be read out by adding cut‐off filter, heating and using ultrafast camera (UCA)/pulsed excitation (PE). This multilevel anti‐counterfeiting label significantly increases the difficulties of imitation meanwhile no expensive equipment is required for the verification of authenticity. Moreover, two bits of binary numbers that can be represented by dual emissions of the Mn:CsPbCl 3 PQDs by changing the doping content, which significantly increases the information storage capacity, are also demonstrated.
Due to The Vast Territory of China, The Topography, Geological Conditions and Surrounding Environment of the Construction Project are Very Different. The Design and Construction of the Foundation Pit Support Project is One of the Most Closely Integrated Engineering Measures Combined with the Above Conditions. The Design and Construction of Deep Foundation Pit Support Engineering in Special Geotechnical Areas Has Become One of the Most Complicated Technical Problems in the Field of Civil Engineering Construction. Based on the Above Background, The Purpose of this Paper is to Study the Foundation Pit Support Structure Under Special Geotechnical Structure. This Paper Compares and Analyzes the Materials and Structure Types that Can be Used for Foundation Pit Support, And Concludes that Bamboo is an Economical and Environmentally Friendly New Material, Its Output Large, Good Mechanical Properties, Can Be Used For Temporary Support of Shallow Foundation Pits. It is Proposed to Use the Bamboo Tube as the Foundation Pit Support Material, And it is Applied in the Soft Soil Layer Foundation Pit Support Project in the form of Row Pile Support Structure. In this Paper, the Top Displacement of the Bamboo Pipe Pile is Larger than that of the Steel Pipe Pile, But the Maximum Pile Top Displacement of the Bamboo Pipe Pile is 1.8mm. The Maximum Displacement of the Pile Top is 18mm, The Displacement is Small, Within the Allowable Range, and in the Actual Project, the Pile Top is Generally Constrained to Further Control the Displacement of the Pile Top to Ensure the Safety and Stability of the Foundation Pit.
Abstract The smart materials with multi‐color and stimuli‐responsive luminescence are very promising for next generation of optical information encryption and anti‐counterfeiting, but these materials are still scarce. Herein, a multi‐level information encryption strategy is developed based on the polychromatic emission of Sb‐doped double perovskite powders (SDPPs). Cs 2 NaInCl 6 :Sb, Cs 2 KInCl 6 :Sb, and Cs 2 AgInCl 6 :Sb synthesized through coprecipitation methods exhibit broadband emissions with bright blue, cyan, and orange colors, respectively. The information transmitted by specific SDPP is encrypted when different SDPPs are mixed. The confidential information can be decrypted by selecting the corresponding narrowband filter. Then, an encrypted quick response (QR) code with improved security is demonstrated based on this multi‐channel selection strategy. Moreover, the three types of SDPPs exhibit three different water‐triggered luminescence switching behaviors. The confidential information represented by Cs 2 NaInCl 6 :Sb can be erased/recovered through a simple water spray/drying. Whereas, the information collected from the green channel is permanently erased by moisture, which fundamentally avoids information leakage. Therefore, different encryption schemes can be designed to meet a variety of encryption requirements. The multicolor and stimuli‐responsive luminescence greatly enrich the flexibility of optical information encryption, which leaps the level of security and confidentiality.
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