Kajal Thakur

Lovely Professional University

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Neha Salaria

Central Potato Research Institute

Umesh Goutam

Lovely Professional University

Sarvjeet Kukreja

Lovely Professional University

Vinay Bhardwaj

Indira Gandhi Medical College

Sundaresha Siddappa

Central Potato Research Institute

Baljeet Singh

Central Potato Research Institute

Maharishi Tomar

Indian Grassland and Fodder Research Institute

Neha Sharma

Central Potato Research Institute

Salej Sood

Central Potato Research Institute

Rahul Bakade

University of Agricultural Sciences, Bangalore

Cooperative Institutions

Central Potato Research Institute

Lovely Professional University

Indian Council of Agricultural Research

Institute of Microbial Technology

Indian Agricultural Research Institute

Panjab University

Indian Institute of Science Bangalore

University of Agricultural Sciences, Dharwad

Chaudhary Charan Singh Haryana Agricultural University

Indira Gandhi Agricultural University

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CRISPR/Cas9-mediated editing of phytoene desaturase (PDS) gene in an important staple crop, potato

3 Biotech (2023)

Sundaresha Siddappa Neha Sharma Neha Salaria Kajal Thakur Shruti Pathania

Phytoene desaturase

Pentatricopeptide repeat

10.1007/s13205-023-03543-w

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Citations (8)

Spraying of dsRNA Molecules Derived from Phytophthora Infestans, as a Plant Protection Strategies for the Management of Potato Late Blight

Sundaresha Siddappa Sanjeev Sharma Aarti Bairwa Maharishi Tomar Ravinder Kumar

Phytophthora. infestans is a well known late blight causing oomycetes pathogen. It evolves and adapts to the host background and new fungicide molecules rapidly within a few years of their release, may be due to the predominance of transposable elements in its genome. Frequent and huge applications of fungicides cause environmental concerns. Here we developed target specific RNA interference based molecules, that are capable of effectively reducing the late blight infection. cDNA microarray expression data was used for the selection of genes involved in the early stage of infection process, sporulation etc. The in vitro synthesis of double stranded RNA molecule, targeting SDH, EF-1α, GPI-HAM344, PLD-3 and HSP-90 encoding genes revealed the reduction in growth, sporulation and symptom expression, which were subsequently assessed by culture bioassay, detached leaf assay and topical application methods. The multiple genes targeted dsRNA nano clay sprayed plants showed enhanced disease resistance (4% disease severity) and least sporulation (<1x103), compared to naked dsRNA spray. Use of nano clay was assumed to be involved in the effective delivery, protection and boosting the action of RNAi in potato plants. A significant difference in the growth, sporulation count, disease severity and reduced expression of the genes and confocal microscopy imaging authenticates the effects of SIGS on late blight disease progression. Our research demonstrated that topical dsRNA nano clay spray under the open-air environment could be an alternative to chemical fungicides and transgenic approaches as a novel plant protection strategy for late blight in an environmentally friendly manner.

Phytophthora infestans

RNA Silencing

Plant disease

10.20944/preprints202102.0280.v1

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Citations (10)

Randomly amplified polymorphic DNA polymorphism in various chicken breeds

Indian Journal of Poultry Science (2008)

Kajal Thakur Rahul Sharma Amrita Ghosh Vijay Kumar Saxena

The RAPD markers were used to detect genetic polymorphism among three chicken breeds i.e., Rhode Island Red (RIR), New Hampshire (NH) and White Leghorn (WLH) by using 10 random decamer primers. Five of the 9 random primers (56%) generated reproducible polymorphism pattern between 3 chicken breeds while other 4 primers (44%) produced monomorphic RAPD patterns. A total of 43 bands were amplified and 36 (83%) of them were polymorphic. The average numbers of the total bands amplified per primer were 5 (RIR), 4 (NH) and 5 (WLH). The RAPD profile of NH on amplification with the primers OPAW11 showed specific bands of molecular size 1897 bp and 702 bp whereas the RAPD profile of RIR on amplification with primer OPAW11, OPBF7 and OPAW3 showed specific bands of molecular size 629 bp, 812 bp and 344 bp, respectively.

Primer (cosmetics)

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Stigma receptivity and pollination success in potato (Solanum tuberosum L.)

Indian Journal of Genetics and Plant Breeding (The) (2005)

P. Manivel Vinod Kumar Kajal Thakur S. K. Pandey

Solanum tuberosum

Receptivity

Stigma

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Citations (2)

Preparation of NanoSilver coated Cotton Fabric and its bactericidal evaluation

G. S. S. Saini Kajal Thakur M. Divya Gnaneswari

We have developed silver nanoparticle coated cotton fabric and evaluated its anti bacterial efficacy. Silver nano particles were synthesized by chemical reduction of silver nitrate using hydrazine hydrate as reducing agent and polyvinyl alcohol as stabilizing agent. It is coated over cotton cloth by ultrasonication method. The anti microbial efficacy has been studied by inhibition zone assay and agar plate count assay against gram negative bacteria. The longevity of anti bacterial property of clothes has also been determined by checking its bactericidal effect after washing the cloth several times by ezee liquid.

Polyvinyl Alcohol

Silver nitrate

Sonication

Hydrazine (antidepressant)

Reducing agent

Silver nanoparticle

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Citations (2)

Fungal Disease Management in Chickpea: Current Status and Future Prospects

Springer eBooks (2018)

Sarvjeet Kukreja Neha Salaria Kajal Thakur Umesh Goutam

Ascochyta

Disease management

10.1007/978-981-13-0393-7_17

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Citations (11)

Functional genomic approaches to improve crop plant heat stress tolerance

F1000Research (2019)

Baljeet Singh Neha Salaria Kajal Thakur Sarvjeet Kukreja Shristy Gautam

Heat stress as a yield limiting issue has become a major threat for food security as global warming progresses. Being sessile, plants cannot avoid heat stress. They respond to heat stress by activating complex molecular networks, such as signal transduction, metabolite production and expressions of heat stress-associated genes. Some plants have developed an intricate signalling network to respond and adapt it. Heat stress tolerance is a polygenic trait, which is regulated by various genes, transcriptional factors, proteins and hormones. Therefore, to improve heat stress tolerance, a sound knowledge of various mechanisms involved in the response to heat stress is required. The classical breeding methods employed to enhance heat stress tolerance has had limited success. In this era of genomics, next generation sequencing techniques, availability of genome sequences and advanced biotechnological tools open several windows of opportunities to improve heat stress tolerance in crop plants. This review discusses the potential of various functional genomic approaches, such as genome wide association studies, microarray, and suppression subtractive hybridization, in the process of discovering novel genes related to heat stress, and their functional validation using both reverse and forward genetic approaches. This review also discusses how these functionally validated genes can be used to improve heat stress tolerance through plant breeding, transgenics and genome editing approaches.

Suppression subtractive hybridization

Molecular breeding

10.12688/f1000research.19840.1

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Citations (37)

Changing Trends in Microalgal Energy Production- Review of Conventional and Emerging Approaches

Journal of Pure and Applied Microbiology (2017)

Sarvjeet Kukreja Kajal Thakur Neha Salaria Umesh Goutam

10.22207/jpam.11.2.42

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Citations (2)

Green Silver Nanoparticles for Phytopathogen Control

Proceedings of the National Academy of Sciences India Section B Biological Sciences (2019)

Nitu Gautam Neha Salaria Kajal Thakur Sarvjeet Kukreja Neha Yadav

Bacillus megaterium

Silver nanoparticle

10.1007/s40011-019-01115-8

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Citations (36)

Solanum tuberosum (CYCLING DOF FACTOR) CDF1.2 allele: A candidate gene for developing earliness in potato

South African Journal of Botany (2020)

Neha Salaria Sundaresha Siddappa Kajal Thakur Maharishi Tomar Umesh Goutam

Circadian clock regulated CDF (CYCLING DOF FACTORs) are a cluster of transcriptional repressors, that accentuates a major quantitative trait locus (QTL) for earliness in potato. Earliness in the plant is a desirable agronomic trait for higher productivity and also enable it to withstand various biotic and abiotic stresses. Since potato is a temperate crop, its tropicalization in the Indian subcontinent is a major agricultural challenge. StCDF1 induce a photoperiod-dependent tuberization pathway by interacting with other molecular regulators such as CONSTANS (StCO1/2) and StSP6A. The present study was conducted to elucidate the allelic variation, expression, role in flowering and tuberization of StCDF1 in contrasting tuberization behaviour of diverse Indian potato cultivars, Kufri Surya (KS) and Kufri Chandramukhi (KCM). Our results confirmed two allelic forms of StCDF1 viz. CDF1.2 and CDF1.3. Their expression profile suggests a higher upregulation of both CDF1.2 and CDF1.3 expression at dawn (6:00 am) than noon (12:00 pm). Virus-Induced Gene Silencing was used to validate the role of these alleles with respect to earliness and tuberization in KS. Real-time confirmed the upregulation of StCO and StSP5G and downregulation of StSP6A in VIGS plants. Phenotypic observations suggested delayed tuberization and a low tuber number in VIGS plants. This present study confirms the allelic variation, the importance and functionality of StCDF1.2 in conferring earliness in tetraploid potato cultivars.

Solanum tuberosum

10.1016/j.sajb.2020.05.008

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Citations (14)