JOSE ANGELO LAULETTA LINDOSO

Universidade de São Paulo

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Eudes Alves Simões Neto

Universidade Federal do Maranhão

MARIANNA MARTINS LAGO

Instituto de Infectologia Emílio Ribas

Aline Santos Ibanês

Instituto de Infectologia Emílio Ribas

MARIA ALMERICE LOPES SILVA

Faculdade de Agudos

LILIAN MITIKO OUKI

Instituto de Infectologia Emílio Ribas

NORIVAL KESPER

Universidade de São Paulo

MAHYUMI FUGIMORI

Universidade de São Paulo

WALTER LINS BARBOSA

Faculdade de Agudos

FERNANDA FERNANDES FONSECA

Ministério da Saúde

BEATRIZ CELESTE

Universidade de São Paulo

Cooperative Institutions

Instituto de Infectologia Emílio Ribas

Universidade de São Paulo

Faculdade de Agudos

Universidade Federal do Maranhão

Hospital São Domingos

Centro Universitário do Norte

Instituto Dante Pazzanese de Cardiologia

Universidade Municipal de São Caetano do Sul

Society for Healthcare Epidemiology of America

Secretaria da Saúde

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Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study

Research Square (Research Square) (2019)

Mirella Alves Cunha BEATRIZ CELESTE NORIVAL KESPER MAHYUMI FUGIMORI MARIANNA MARTINS LAGO

Abstract Background: There is little information about the frequency of Leishmaniainfection in asymptomatic HIV infected individuals and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmaniaspp. infection in HIV infected patients living in an urban area from Brazil. Methods: To detect Leishmaniainfection, were performed diagnostic tests to detection of antibodies anti-Leishmania(ELISA using Leptomonas seymouriantigens; ELISA using rk39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT) and detection of LeishmaniaDNA by polymerase chain reaction (PCR) with the target genes kDNA and ITS-1. Results: Frequency, considering at least one positive test, was 15%. For ELISA using Leptomonasantigens and IFAT, there was an association between CD4+ T-lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (T CD4+ cells count < 200/mm3). Conclusions: According to our data, there was a high prevalence of Leishmaniaspp. in this population living with HIV. Although there is the possibility of cross-reaction, some tests considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of T CD4+ lymphocytes.

Direct agglutination test

Cross-sectional study

Direct fluorescent antibody

10.21203/rs.2.18499/v1

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Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study

Research Square (Research Square) (2020)

Mirella Alves Cunha BEATRIZ CELESTE NORIVAL KESPER MAHYUMI FUGIMORI MARIANNA MARTINS LAGO

Abstract Background: There is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmania spp. infection in HIV-infected patients living in an urban area in Brazil.Methods: To detect Leishmania infection, diagnostic tests were performed to detect anti- Leishmania antibodies (ELISA using Leptomonas seymouri antigens; ELISA using rK39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT)) and Leishmania DNA (polymerase chain reaction (PCR) with the target genes kDNA and ITS-1).Results: The frequency of at least one positive test was 15%. For ELISA using Leptomonas antigens and IFAT, there was an association between CD4+ T lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (CD4+ T cell count < 200/mm 3 ).Conclusions: According to our data, there was a high prevalence of Leishmania spp. infections in this population living with HIV. Although there is the possibility of cross-reaction, some tests that are considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of CD4+ T lymphocytes.

Direct agglutination test

10.21203/rs.2.18499/v3

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Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study

Research Square (Research Square) (2020)

Mirella Alves Cunha BEATRIZ CELESTE NORIVAL KESPER MAHYUMI FUGIMORI MARIANNA MARTINS LAGO

Abstract Background: There is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmania spp. infection in HIV-infected patients living in an urban area in Brazil. Methods: To detect Leishmania infection, diagnostic tests were performed to detect anti- Leishmania antibodies (ELISA using Leptomonas seymouri antigens; ELISA using rK39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT)) and Leishmania DNA (polymerase chain reaction (PCR) with the target genes kDNA and ITS-1). Results: The frequency of at least one positive test was 15%. For ELISA using Leptomonas antigens and IFAT, there was an association between CD4+ T lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (CD4+ T cell count < 200/mm 3 ). Conclusions: According to our data, there was a high prevalence of Leishmania spp. infections in this population living with HIV. Although there is the possibility of cross-reaction, some tests that are considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of CD4+ T lymphocytes.

Direct agglutination test

10.21203/rs.2.18499/v2

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