FÖ

F. Özdal-Kurt

Manisa Celal Bayar University

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Bilge Hakan Şen

Izmir University

Mİ

Mehmet İbrahim Tuğlu

Manisa Celal Bayar University

D. Ila

Fayetteville State University

İD

İsmet Deliloğlu-Gürhan

Ege University

İG

İsmet Gürhan

Inonu University

C. Muntele

Cygnus (United States)

Richard K. Zimmerman

University of Pittsburgh

Marcela Charantola Rodrigues

Forest Science and Research Institute

SI Deliloğlu-Gürhan

Izmir Kâtip Çelebi University

H. Seda Vatansever

Manisa Celal Bayar University

Cooperative Institutions

Ege University

Manisa Celal Bayar University

Alabama Agricultural and Mechanical University

Izmir Institute of Technology

Texas A&M University

Near East University

Sağlık Bilimleri Üniversitesi

Hacettepe University

Ankara University

Institute of High Current Electronics

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The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction

Biotechnic & Histochemistry (2015)

F. Özdal-Kurt Mehmet İbrahim Tuğlu H. Seda Vatansever Shunyi Tong SI Deliloğlu-Gürhan

Bone marrow contains mesenchymal stem cells that form many tissues. Various scaffolds are available for bone reconstruction by tissue engineering. Osteoblastic differentiated bone marrow stromal cells (BMSC) promote osteogenesis on scaffolds and stimulate bone regeneration. We investigated the use of cultured autologous BMSC on different scaffolds for healing defects in tibias of adult male canines. BMSC were isolated from canine humerus bone marrow, differentiated into osteoblasts in culture and loaded onto porous ceramic scaffolds including hydroxyapatite 1, hydroxyapatite gel and calcium phosphate. Osteoblast differentiation was verified by osteonectine and osteocalcine immunocytochemistry. The scaffolds with stromal cells were implanted in the tibial defect. Scaffolds without stromal cells were used as controls. Sections from the defects were processed for histological, ultrastructural, immunohistochemical and histomorphometric analyses to analyze the healing of the defects. BMSC were spread, allowed to proliferate and differentiate to osteoblasts as shown by alizarin red histochemistry, and osteocalcine and osteonectine immunostaining. Scanning electron microscopy showed that BMSC on the scaffolds were more active and adhesive to the calcium phosphate scaffold compared to the others. Macroscopic bone formation was observed in all groups, but scaffolds with stromal cells produced significantly better results. Bone healing occurred earlier and faster with stromal cells on the calcium phosphate scaffold and produced more callus compared to other scaffolds. Tissue healing and osteoblastic marker expression also were better with stromal cells on the scaffolds. Increased trabecula formation, cell density and decreased fibrosis were observed in the calcium phosphate scaffold with stromal cells. Autologous cultured stromal cells on the scaffolds were useful for healing of canine tibial bone defects. The calcium phosphate scaffold was the best for both cell differentiation in vitro and bone regeneration in vivo. It may be possible to improve healing of bone defects in humans using stem cells from bone marrow.

10.3109/10520295.2014.983547

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Citations (8)

Neural Cell Attachment on Metal Ion Implanted Glass Surfaces

MRS Proceedings (2011)

Emel Sokullu Ahmet Öztarhan İsmet Deliloğlu-Gürhan Sultan Gülçe İz F. Özdal-Kurt

Vacuum arc

Surface Modification

Neural cell

10.1557/opl.2011.1471

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Citations (0)

WITHDRAWN: Cell adhesion study of the titanium alloys exposed to glow discharge

Nuclear Instruments and Methods in Physics Research Section B Beam Interactions with Materials and Atoms (2007)

V. Abidzina İsmet Deliloğlu-Gürhan F. Özdal-Kurt Bilge Hakan Şen I. Tereshko

Accidental

Glow discharge

10.1016/j.nimb.2007.04.153

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Neurotoxic effect of Caulerpa racemosa var. cylindracea by neurite inhibition on the neuroblastoma cell line

Russian Journal of Marine Biology (2009)

Oğuz Kurt F. Özdal-Kurt Mehmet İbrahim Tuğlu SI Deliloğlu-Gürhan Mehmet Öztürk

Neurite

10.1134/s1063074009040105

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Citations (8)

Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines

Acta Histochemica (2009)

H. Seda Vatansever Kadriye Sorkun S. İsmet Gürhan F. Özdal-Kurt Elgin Türköz

Propolis

Breast carcinoma

10.1016/j.acthis.2009.06.001

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Citations (81)

Apoptotic Properties of Rutheinum Complexes on Different Type of Cancer Cell Lines

Seda Vatansever Hilal Kabadayı Mehmet Korkmaz F. Özdal-Kurt Serdar Batıkan Kavukcu

Among chemotherapeutic agents, cisplatin and the other platinum-based drugs have occupied for 35 years an enviable position. The limitations of platinum-based drugs, dose dependent side effects and development of drug resistance mechanisms, have boosted the research for finding other metal-based drugs. Among metals, ruthenium is probably the one showing the greatest promises. Ruthenium (Ru) appears to be less toxic than platinum and several biological studies have indicated that ruthenium complexes possess diverse modes of action. The redox chemistry of ruthenium is rich and compatible with biological media, and the overall toxicity of ruthenium is lower than platinum, thus allowing higher doses of treatment. In this study we aimed that, analyses of different type of ruthenium complexes in cancer cell lines. Six Ru complexes were determined by elemental analysis, FTIR, NMR, UV-visible spectroscopy, electron density on the metal was measured by cyclic voltammetry. After that, the cellular properties of this complexes were analyses on PC-3, HT-29, Du-145 and Vero cell lines. DNA damage was analyzed H2AX staining, apoptotic cell analyses were performed flow cytometry and western blotting. After 48 h incubation of Ru complexes three of them more effective for cell lines. Especially Ru3 was more effective in cancer cell lines. Apoptotic pathway was triggered after Ru complexes incubation in PC-3, Du-145 and Ht-29 cancer cell lines. Our study suggest that Ru complexes may be used for cancer cell cytotoxicity as a drugs in patients.

10.3390/proceedings2251593

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Citations (3)

The cytotoxic, neurotoxic, apoptotic and antiproliferative activities of extracts of some marine algae on the MCF-7 cell line

Biotechnic & Histochemistry (2014)

Oğuz Kurt F. Özdal-Kurt MI Tuğlu CM Akçora

We investigated the cytotoxic, neurotoxic, apoptotic and antiproliferative effects of extracts from Petalonia fascia, Jania longifurca and Halimeda tuna on the MCF-7 breast cancer cell line. J. longifurca extracts were more toxic than those of P. fascia and H. tuna. The algal extracts showed significant toxic effects at different dilutions. The toxic effects were due to increased oxidative stress and resulted in apoptosis. Algal toxicity may exert negative effects through the food chain or by direct interaction. Algal toxicity also has potential for cancer therapy. The toxic effects that we observed may be especially important for therapy for breast tumors.

MCF-7

10.3109/10520295.2014.917199

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Citations (21)

The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite.

PubMed (2006)

İsmet Deliloğlu-Gürhan İbrahim Tuğlu Seda Vatansever F. Özdal-Kurt Hayati Ekren

To investigate the adhesive properties of bone marrow stromal cell (BMSC) on the hydroxyapatite (HA) particles and analyze their behavior.The study took place in the Department of the Histology and Embryology, Celal Bayar University, Manisa and in the Department of Bioengineering, Ege University, Izmir, Turkey between 2004 and 2005. We cultured BMSC from the mature rat tibia and differentiated to the osteoblasts by osteogenic medium. The BMSCs were subcultured and were taken to the HA substrate. We measured their proliferation capacity and viability with MTT assay using the spectrophotometric method. Furthermore, we identified the osteoblast-like cells by immunohistochemical staining of osteonectin and osteocalcin and we analyzed the behavior of the cells on different sized HA particles by SEM at the end of 3 days incubation.Osteogenic medium caused the proliferation capacity of BMSC to speed up and the effects appeared earlier. We confirmed the osteoblastic differentiation by staining of most cells with osteoblastic markers. Subcultured cells were similarly adhesive to the HA particles and the osteogenic medium did not alter this behavior. They spread on the substrate similarly. Most of the cells demonstrated the cytoplasmic protrusion. Morphology of the cells did not change much with or without osteogenic medium. Different sizes of HA particles did not affect the adhesive properties of these cells except HA gel. The spreading and attachment ratios of the cells on HA gel were more than the othersWe found that there was heterogeneity in BMSC on differentiation capacity to the osteoblast, which was a sign of a subpopulation. Adhesive cells showed similar morphology and behavior under the effect of osteogenic medium. The only difference was the spreading capacity on the HA gel where cell used this substrate more effectively for adhesion.

Osteonectin

MTT assay

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Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system

Acta Histochemica (2006)

SI Deliloğlu-Gürhan H. Seda Vatansever F. Özdal-Kurt Mehmet İbrahim Tuğlu

Osteonectin

10.1016/j.acthis.2005.11.001

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Citations (41)