Patrícia Aparecida da Silva

Universidade Federal de Juiz de Fora

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Marcone Augusto Leal de Oliveira

Universidade Federal de Juiz de Fora

Ana Carolina Oliveira Costa

Universidade Federal de Santa Catarina

Melina Heller

Centro de Pesquisas Oncológicas

Leônidas Paixão Passos

Brazilian Agricultural Research Corporation

Gustavo Amadeu Micke

Universidade Federal de Santa Catarina

Fernando Antônio Simas Vaz

Universidade Federal Fluminense

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Universidade Federal de Santa Catarina

Universidade Federal de Juiz de Fora

Brazilian Agricultural Research Corporation

Centro de Ensino Superior de Juiz de Fora

Universidade Estadual de Campinas (UNICAMP)

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Brazilian Biosciences National Laboratory

Universidade Federal do Rio de Janeiro

Laboratoire de Synthèse Organique

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Optimisation of a Capillary Zone Electrophoresis Methodology for Simultaneous Analysis of Organic Aliphatic Acids in Extracts of Brachiaria brizantha

Phytochemical Analysis (2012)

Fernando Antônio Simas Vaz Patrícia Aparecida da Silva Leônidas Paixão Passos Melina Heller Gustavo Amadeu Micke

ABSTRACT Introduction Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl‐ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); −15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha , demonstrating its usefulness to study aluminum tolerance. Copyright © 2012 John Wiley & Sons, Ltd.

Malic acid

Organic acid

Oxalic Acid

Succinic acid

Repeatability

Central composite design

Tartaric acid

10.1002/pca.2355

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Citations (13)