Abstract Abstract : (Background)Liaoning cashmere goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)This study investigated how LncRNA mediates the effects of FGF5 on the growth of Liaoning cashmere goats. We screened for LncRNA related to hair follicle development and villus growth by RNA-seq sequencing. GO and pathway analysis determined that the optimal treatment conditions for FGF5 drugs are 10 -4 g/L for 72h (F4_72h). The expression levels of CBS, CTH, keratin gene K26, KAP11.1 were studied when overexpressing and interfering with LncRNA. (Conclusions)To our knowledge, this is the first study on how LncRNA regulates villi growth by regulating target genes and keratin genes in the amino acid metabolic pathway; it is also the first to open a new research direction for studying the mechanism of FGF5 in regulating hair follicle development and villus growth.
Abstract (Background) Liaoning Cashmere Goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results) We demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Overexpressing LncRNA reversed the inhibiting effect of FGF5 on the target genes CBS and CTH. (Conclusions) we believe that FGF5 can regulate the growth and development of Cashmere Goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.
Abstract (Background)Liaoning Cashmere Goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)We demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Overexpressing LncRNA reversed the inhibiting effect of FGF5 on the target genes CBS and CTH. (Conclusions)we believe that FGF5 can regulate the growth and development of Cashmere Goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.
Abstract (Background)Liaoning cashmere goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results)This study investigated how LncRNA mediates the effects of FGF5 on the growth of Liaoning cashmere goats. By using RNA-seq sequencing technology, over-expression and interference lentiviral technology and qPCR, we demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Then, overexpressing LncRNA in skin cells reversed the inhibiting effect of FGF5 on the target genes CBS and CTH which further inhibited the expression of k26, kap11.1. Finally, we demonstrated the inhibition of CBS and CTH and elevation k26 and kap11.1 genes when the expression level of LncRNA gene is downregulated using RNA interference. (Conclusions)Therefore, we believe that FGF5 can regulate the growth and development of cashmere goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.
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